Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology

Keisuke Iso; Takashi K. Saito; Hitoshi Muguruma; Hitoshi Tabata; Satoshi Konishi

Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology

Keisuke Iso, Takashi K. Saito, Hitoshi Muguruma, Hitoshi Tabata, Satoshi Konishi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper reports the design, fabrication, and experimental results for a Micro Channel Array (MCA)-based photodynamic cell perforator toward intracellular technology. The perforator will be developed to improve whole-cell patch-clamp technique. The perforator consists of planar micro channels and electrodes of the MCA. The perforator utilizes the micro channel for both cell suction and chemical supply. The diameter of the micro channel is Φ2 μm. The photosensitizer (PS) is introduced to the cell membrane through the micro channel, and then the cell membrane is perforated photodynamically by activated PS. Photodynamic perforation of the cell membrane performed successfully in the experiment using PC 12 cells with diameters as small as tens of μm.

Original language	English
Title of host publication	Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
Pages	445-448
Number of pages	4
Publication status	Published - 2007
Event	20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan Duration: 2007 Jan 21 → 2007 Jan 25

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
Country/Territory	Japan
City	Kobe
Period	07/1/21 → 07/1/25

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Cite this

Iso, K., Saito, T. K., Muguruma, H., Tabata, H., & Konishi, S. (2007). Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. In Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007 (pp. 445-448). [4433067] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. / Iso, Keisuke; Saito, Takashi K.; Muguruma, Hitoshi et al.

Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. 2007. p. 445-448 4433067 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Iso, K, Saito, TK, Muguruma, H, Tabata, H & Konishi, S 2007, Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. in Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007., 4433067, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 445-448, 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007, Kobe, Japan, 07/1/21.

Iso K, Saito TK, Muguruma H, Tabata H, Konishi S. Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. In Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. 2007. p. 445-448. 4433067. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Iso, Keisuke ; Saito, Takashi K. ; Muguruma, Hitoshi et al. / Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. 2007. pp. 445-448 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "This paper reports the design, fabrication, and experimental results for a Micro Channel Array (MCA)-based photodynamic cell perforator toward intracellular technology. The perforator will be developed to improve whole-cell patch-clamp technique. The perforator consists of planar micro channels and electrodes of the MCA. The perforator utilizes the micro channel for both cell suction and chemical supply. The diameter of the micro channel is Φ2 μm. The photosensitizer (PS) is introduced to the cell membrane through the micro channel, and then the cell membrane is perforated photodynamically by activated PS. Photodynamic perforation of the cell membrane performed successfully in the experiment using PC 12 cells with diameters as small as tens of μm.",

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AB - This paper reports the design, fabrication, and experimental results for a Micro Channel Array (MCA)-based photodynamic cell perforator toward intracellular technology. The perforator will be developed to improve whole-cell patch-clamp technique. The perforator consists of planar micro channels and electrodes of the MCA. The perforator utilizes the micro channel for both cell suction and chemical supply. The diameter of the micro channel is Φ2 μm. The photosensitizer (PS) is introduced to the cell membrane through the micro channel, and then the cell membrane is perforated photodynamically by activated PS. Photodynamic perforation of the cell membrane performed successfully in the experiment using PC 12 cells with diameters as small as tens of μm.

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Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology

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