Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology

Keisuke Iso, Takashi K. Saito, Hitoshi Muguruma, Hitoshi Tabata, Satoshi Konishi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper reports the design, fabrication, and experimental results for a Micro Channel Array (MCA)-based photodynamic cell perforator toward intracellular technology. The perforator will be developed to improve whole-cell patch-clamp technique. The perforator consists of planar micro channels and electrodes of the MCA. The perforator utilizes the micro channel for both cell suction and chemical supply. The diameter of the micro channel is Φ2 μm. The photosensitizer (PS) is introduced to the cell membrane through the micro channel, and then the cell membrane is perforated photodynamically by activated PS. Photodynamic perforation of the cell membrane performed successfully in the experiment using PC 12 cells with diameters as small as tens of μm.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages445-448
Number of pages4
Publication statusPublished - 2007
Event20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe
Duration: 2007 Jan 212007 Jan 25

Other

Other20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
CityKobe
Period07/1/2107/1/25

Fingerprint

Perforators
perforation
Cell membranes
Photosensitizing Agents
Photosensitizers
cells
Clamping devices
clamps
suction
Fabrication
Electrodes
fabrication
electrodes
Experiments

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Iso, K., Saito, T. K., Muguruma, H., Tabata, H., & Konishi, S. (2007). Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 445-448). [4433067]

Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. / Iso, Keisuke; Saito, Takashi K.; Muguruma, Hitoshi; Tabata, Hitoshi; Konishi, Satoshi.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2007. p. 445-448 4433067.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Iso, K, Saito, TK, Muguruma, H, Tabata, H & Konishi, S 2007, Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 4433067, pp. 445-448, 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007, Kobe, 07/1/21.
Iso K, Saito TK, Muguruma H, Tabata H, Konishi S. Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2007. p. 445-448. 4433067
Iso, Keisuke ; Saito, Takashi K. ; Muguruma, Hitoshi ; Tabata, Hitoshi ; Konishi, Satoshi. / Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2007. pp. 445-448
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