Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology

Keisuke Iso, Takashi K. Saito, Hitoshi Muguruma, Hitoshi Tabata, Satoshi Konishi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper reports the design, fabrication, and experimental results for a Micro Channel Array (MCA)-based photodynamic cell perforator toward intracellular technology. The perforator will be developed to improve whole-cell patch-clamp technique. The perforator consists of planar micro channels and electrodes of the MCA. The perforator utilizes the micro channel for both cell suction and chemical supply. The diameter of the micro channel is Φ2 μm. The photosensitizer (PS) is introduced to the cell membrane through the micro channel, and then the cell membrane is perforated photodynamically by activated PS. Photodynamic perforation of the cell membrane performed successfully in the experiment using PC 12 cells with diameters as small as tens of μm.

Original languageEnglish
Title of host publicationProceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
Pages445-448
Number of pages4
Publication statusPublished - 2007 Dec 1
Event20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan
Duration: 2007 Jan 212007 Jan 25

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

Conference20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
CountryJapan
CityKobe
Period07/1/2107/1/25

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Iso, K., Saito, T. K., Muguruma, H., Tabata, H., & Konishi, S. (2007). Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology. In Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007 (pp. 445-448). [4433067] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).