Abstract
The photogeneration of nicotinamide adenine dinucleotide hydrophosphate (NADPH) and its associated reaction were studied in a novel photo-energy conversion system. The system was composed of the oligothiophene, dimethyl- 4,4'-bipyridinium (MV2+), EDTA, and combined with ferredoxin-NADP reductase (FDR, E.C.1.18.1.2). The NADPH was generated by the FDR catalysis via the reduction of MV2+ by the photocatalysis of oligothiophene and EDTA as the sacrificial electron donor. As a result, the rate of the NADPH generation depended on the concentrations of nicotinamide adenine dinucleotide phosphate (NADP+) and MV2+. The effect of the intension of applied light showed the same tendency. Rate values of photoreduction of MV2+ and photogeneration of NADPH systems were investigated as a function of the concentration of MV2+. The production rates of methyl viologen radical cation (MV+) were a 1000- fold more than the NADPH production rates in the oligothiophenes. These results suggest that the electron transfer between FDR and its substrates is the rate-determining step.
| Original language | English |
|---|---|
| Pages (from-to) | 213-220 |
| Number of pages | 8 |
| Journal | Journal of Biotechnology |
| Volume | 59 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1998 Jan 3 |
| Externally published | Yes |
Fingerprint
Keywords
- EDTA
- Ferredoxin-NADP reductase (FDR) catalysis
- Methyl viologen
- NADPH
- Oligothiophene
- Photogeneration
ASJC Scopus subject areas
- Biotechnology
Cite this
Photogeneration of NADPH by oligothiophenes coupled with ferredoxin- NADP reductase. / Kim, Y.; Ikebukuro, K.; Muguruma, Hitoshi; Karube, I.
In: Journal of Biotechnology, Vol. 59, No. 3, 03.01.1998, p. 213-220.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Photogeneration of NADPH by oligothiophenes coupled with ferredoxin- NADP reductase
AU - Kim, Y.
AU - Ikebukuro, K.
AU - Muguruma, Hitoshi
AU - Karube, I.
PY - 1998/1/3
Y1 - 1998/1/3
N2 - The photogeneration of nicotinamide adenine dinucleotide hydrophosphate (NADPH) and its associated reaction were studied in a novel photo-energy conversion system. The system was composed of the oligothiophene, dimethyl- 4,4'-bipyridinium (MV2+), EDTA, and combined with ferredoxin-NADP reductase (FDR, E.C.1.18.1.2). The NADPH was generated by the FDR catalysis via the reduction of MV2+ by the photocatalysis of oligothiophene and EDTA as the sacrificial electron donor. As a result, the rate of the NADPH generation depended on the concentrations of nicotinamide adenine dinucleotide phosphate (NADP+) and MV2+. The effect of the intension of applied light showed the same tendency. Rate values of photoreduction of MV2+ and photogeneration of NADPH systems were investigated as a function of the concentration of MV2+. The production rates of methyl viologen radical cation (MV+) were a 1000- fold more than the NADPH production rates in the oligothiophenes. These results suggest that the electron transfer between FDR and its substrates is the rate-determining step.
AB - The photogeneration of nicotinamide adenine dinucleotide hydrophosphate (NADPH) and its associated reaction were studied in a novel photo-energy conversion system. The system was composed of the oligothiophene, dimethyl- 4,4'-bipyridinium (MV2+), EDTA, and combined with ferredoxin-NADP reductase (FDR, E.C.1.18.1.2). The NADPH was generated by the FDR catalysis via the reduction of MV2+ by the photocatalysis of oligothiophene and EDTA as the sacrificial electron donor. As a result, the rate of the NADPH generation depended on the concentrations of nicotinamide adenine dinucleotide phosphate (NADP+) and MV2+. The effect of the intension of applied light showed the same tendency. Rate values of photoreduction of MV2+ and photogeneration of NADPH systems were investigated as a function of the concentration of MV2+. The production rates of methyl viologen radical cation (MV+) were a 1000- fold more than the NADPH production rates in the oligothiophenes. These results suggest that the electron transfer between FDR and its substrates is the rate-determining step.
KW - EDTA
KW - Ferredoxin-NADP reductase (FDR) catalysis
KW - Methyl viologen
KW - NADPH
KW - Oligothiophene
KW - Photogeneration
UR - http://www.scopus.com/inward/record.url?scp=0032477488&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032477488&partnerID=8YFLogxK
U2 - 10.1016/S0168-1656(97)00155-7
DO - 10.1016/S0168-1656(97)00155-7
M3 - Article
AN - SCOPUS:0032477488
VL - 59
SP - 213
EP - 220
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 3
ER -