Photogeneration of NADPH by oligothiophenes coupled with ferredoxin- NADP reductase

Y. Kim, K. Ikebukuro, H. Muguruma, I. Karube

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7 Citations (Scopus)

Abstract

The photogeneration of nicotinamide adenine dinucleotide hydrophosphate (NADPH) and its associated reaction were studied in a novel photo-energy conversion system. The system was composed of the oligothiophene, dimethyl- 4,4'-bipyridinium (MV2+), EDTA, and combined with ferredoxin-NADP reductase (FDR, E.C.1.18.1.2). The NADPH was generated by the FDR catalysis via the reduction of MV2+ by the photocatalysis of oligothiophene and EDTA as the sacrificial electron donor. As a result, the rate of the NADPH generation depended on the concentrations of nicotinamide adenine dinucleotide phosphate (NADP+) and MV2+. The effect of the intension of applied light showed the same tendency. Rate values of photoreduction of MV2+ and photogeneration of NADPH systems were investigated as a function of the concentration of MV2+. The production rates of methyl viologen radical cation (MV+) were a 1000- fold more than the NADPH production rates in the oligothiophenes. These results suggest that the electron transfer between FDR and its substrates is the rate-determining step.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalJournal of Biotechnology
Volume59
Issue number3
DOIs
Publication statusPublished - 1998 Jan 3

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Keywords

  • EDTA
  • Ferredoxin-NADP reductase (FDR) catalysis
  • Methyl viologen
  • NADPH
  • Oligothiophene
  • Photogeneration

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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