Phototoxic process after rapid photosensitive membrane damage of 5,5″-bis(aminomethyl)-2,2′: 5′,2″-terthiophene dihydrochloride

Takashi K. Saito, Mareyuki Takahashi, Hitoshi Muguruma, Etsuo Niki, Kunihiko Mabuchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We report a new aspect of rapid (<30 s) light-induced cell membrane damage photosensitized by 5,5″-bis(aminomethyl)-2,2′:5′,2″-terthiophene dihydrochloride (BAT), which is a water-soluble α-terthienyl analogue, using a high-power laser (light intensity 1.6 W cm-2). In this paper, we will discuss the relationship between the exposure time of the cells to the photosensitizer and the phototoxic process. Three toxic processes can be identified: first, a non-light-mediated toxicity dependent on BAT-cell incubation; second, a phototoxicity independent of BAT exposure time when the BAT concentration is in the 2-10-μM range; third, a phototoxicity dependent on BAT exposure time when BAT concentration becomes 20 μM. The cytotoxicity decreases when α-tocopherol, an antioxidant, is added to a cell membrane. This pattern of phototoxicity is the typical of a phospholipid peroxidation chain reaction and oxidative damage of membrane proteins triggered by a reactive oxygen species generated by a triplet state of BAT. The BAT exposure time is clearly correlated with the partition of the photosensitizer in the cell membrane and inside the cell.

Original languageEnglish
Pages (from-to)114-121
Number of pages8
JournalJournal of Photochemistry and Photobiology B: Biology
Volume61
Issue number3
DOIs
Publication statusPublished - 2001 Aug 30

Fingerprint

Cell membranes
phototoxicity
exposure duration
Photosensitizing Agents
Photosensitizers
membranes
damage
Membranes
cell membranes
Tocopherols
Poisons
cells
Phospholipids
High power lasers
Cytotoxicity
Antioxidants
tocopherol
Toxicity
Reactive Oxygen Species
peroxidation

Keywords

  • α-Terthienyl derivative
  • Antioxidant
  • Phototoxin
  • Reactive oxygen species

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Phototoxic process after rapid photosensitive membrane damage of 5,5″-bis(aminomethyl)-2,2′ : 5′,2″-terthiophene dihydrochloride. / Saito, Takashi K.; Takahashi, Mareyuki; Muguruma, Hitoshi; Niki, Etsuo; Mabuchi, Kunihiko.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 61, No. 3, 30.08.2001, p. 114-121.

Research output: Contribution to journalArticle

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