Phylogeny of Bacterial Symbionts of the Leguminous Tree Acacia mangium

Sukma Nuswantara, Makoto Fujie, Harmastini I. Sukiman, Mitsuo Yamashita, Takashi Yamada, Yoshikatsu Murooka

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Bacterial strains isolated from root nodules on Acacia mangium at various locations in Indonesia were characterized by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified 16S rDNA and by an intergenic repetitive extragenic palindromic (REP) sequence. The RFLP-16S rDNA analysis used four restriction endonucleases classified into seven 5au3AI, eight Taql, nine Hint}, and nine Mspl groups. Analysis of the amplified REP-PCR products showed infra-specific variations in the rhizobial genomic DNA. From the data obtained, 10 of the 15 strains isolated from A. mangium were classified as Bradyrhizobium. These isolates clustered with B. elkanii, and were predominant in the A. mangium nodulation systems. Partial 16S rDNA sequence analysis of some representative strains demonstrated that the RFLP clusters were stable and phylogenetically relevant.

Original languageEnglish
Pages (from-to)511-518
Number of pages8
JournalJournal of Fermentation and Bioengineering
Issue number6
Publication statusPublished - 1997
Externally publishedYes


  • 16S rDNA
  • Acasia mangium
  • Bradyrhizobium elkanii
  • REP
  • RFLP

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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