PKC phosphorylates MARCKS Ser159 not only directly but also through RhoA/ROCK

Atsuhiro Tanabe, Yukinori Kamisuki, Hiroyoshi Hidaka, Masaaki Suzuki, Manabu Negishi, Yoh Takuwa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

It is well recognized that phorbol 12,13-dibutyrate (PDBu)-activated PKC directly phosphorylates myristoylated alanine-rich C kinase substrate (MARCKS), whose phosphorylation is used as a marker of PKC activation. However, in SH-SY5Y neuroblastoma cells, Western blotting analyses revealed that Rho-associated coiled-coil kinase (ROCK)-specific inhibitor H-1152 inhibited PDBu-induced phosphorylation, and that a small G-protein inhibitor, toxin B, also inhibited MARCKS phosphorylation. Furthermore, in GST pull-down assays, PDBu induced RhoA activation in SH-SY5Y cells, and this activation was inhibited by PKC inhibitor Ro-31-8220. Finally, we showed that the transfection of a dominant negative form of RhoA inhibited PDBu-induced MARCKS phosphorylation in immunocytochemistries. These findings suggest that some PDBu-induced MARCKS phosphorylation includes the RhoA/ROCK pathway in SH-SY5Y cells.

Original languageEnglish
Pages (from-to)156-161
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume345
Issue number1
DOIs
Publication statusPublished - 2006 Jun 23
Externally publishedYes

Fingerprint

Phorbol 12,13-Dibutyrate
rho-Associated Kinases
Phosphorylation
Chemical activation
Monomeric GTP-Binding Proteins
Assays
myristoylated alanine-rich C kinase substrate

Keywords

  • MARCKS
  • Neuroblastoma
  • Phosphorylation
  • PKC
  • RhoA
  • ROCK

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

PKC phosphorylates MARCKS Ser159 not only directly but also through RhoA/ROCK. / Tanabe, Atsuhiro; Kamisuki, Yukinori; Hidaka, Hiroyoshi; Suzuki, Masaaki; Negishi, Manabu; Takuwa, Yoh.

In: Biochemical and Biophysical Research Communications, Vol. 345, No. 1, 23.06.2006, p. 156-161.

Research output: Contribution to journalArticle

Tanabe, Atsuhiro ; Kamisuki, Yukinori ; Hidaka, Hiroyoshi ; Suzuki, Masaaki ; Negishi, Manabu ; Takuwa, Yoh. / PKC phosphorylates MARCKS Ser159 not only directly but also through RhoA/ROCK. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 345, No. 1. pp. 156-161.
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AU - Negishi, Manabu

AU - Takuwa, Yoh

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