Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

Dai Kato, Atsumu Oda, Mutsuo Tanaka, Seiichiro Iijima, Tomoyuki Kamata, Masami Todokoro, Yasuo Yoshimi, Osamu Niwa

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.

Original languageEnglish
Pages (from-to)618-624
Number of pages7
JournalElectroanalysis
Volume26
Issue number3
DOIs
Publication statusPublished - 2014 Mar

Keywords

  • Electrochemical detection
  • Lipopolysaccharide
  • Nanocarbon film electrode
  • Poly-ε-lysine
  • Signal amplification

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Fingerprint

Dive into the research topics of 'Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection'. Together they form a unique fingerprint.

Cite this