Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

Dai Kato; Atsumu Oda; Mutsuo Tanaka; Seiichiro Iijima; Tomoyuki Kamata; Masami Todokoro; Yasuo Yoshimi; Osamu Niwa

doi:10.1002/elan.201300542

Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

Dai Kato, Atsumu Oda, Mutsuo Tanaka, Seiichiro Iijima, Tomoyuki Kamata, Masami Todokoro, Yasuo Yoshimi, Osamu Niwa

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe²⁺ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.

Original language	English
Pages (from-to)	618-624
Number of pages	7
Journal	Electroanalysis
Volume	26
Issue number	3
DOIs	https://doi.org/10.1002/elan.201300542
Publication status	Published - 2014 Mar 1

Keywords

Electrochemical detection
Lipopolysaccharide
Nanocarbon film electrode
Poly-ε-lysine
Signal amplification

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

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Kato, D., Oda, A., Tanaka, M., Iijima, S., Kamata, T., Todokoro, M., Yoshimi, Y., & Niwa, O. (2014). Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection. Electroanalysis, 26(3), 618-624. https://doi.org/10.1002/elan.201300542

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abstract = "We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.",

keywords = "Electrochemical detection, Lipopolysaccharide, Nanocarbon film electrode, Poly-ε-lysine, Signal amplification",

author = "Dai Kato and Atsumu Oda and Mutsuo Tanaka and Seiichiro Iijima and Tomoyuki Kamata and Masami Todokoro and Yasuo Yoshimi and Osamu Niwa",

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AU - Kato, Dai

AU - Oda, Atsumu

AU - Tanaka, Mutsuo

AU - Iijima, Seiichiro

AU - Kamata, Tomoyuki

AU - Todokoro, Masami

AU - Yoshimi, Yasuo

AU - Niwa, Osamu

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AB - We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.

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KW - Lipopolysaccharide

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KW - Poly-ε-lysine

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