Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

Dai Kato, Atsumu Oda, Mutsuo Tanaka, Seiichiro Iijima, Tomoyuki Kamata, Masami Todokoro, Yasuo Yoshimi, Osamu Niwa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.

Original languageEnglish
Pages (from-to)618-624
Number of pages7
JournalElectroanalysis
Volume26
Issue number3
DOIs
Publication statusPublished - 2014 Mar

Fingerprint

Lysine
Lipopolysaccharides
Electrodes
Polymyxin B
Ions

Keywords

  • Electrochemical detection
  • Lipopolysaccharide
  • Nanocarbon film electrode
  • Poly-ε-lysine
  • Signal amplification

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Kato, D., Oda, A., Tanaka, M., Iijima, S., Kamata, T., Todokoro, M., ... Niwa, O. (2014). Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection. Electroanalysis, 26(3), 618-624. https://doi.org/10.1002/elan.201300542

Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection. / Kato, Dai; Oda, Atsumu; Tanaka, Mutsuo; Iijima, Seiichiro; Kamata, Tomoyuki; Todokoro, Masami; Yoshimi, Yasuo; Niwa, Osamu.

In: Electroanalysis, Vol. 26, No. 3, 03.2014, p. 618-624.

Research output: Contribution to journalArticle

Kato, D, Oda, A, Tanaka, M, Iijima, S, Kamata, T, Todokoro, M, Yoshimi, Y & Niwa, O 2014, 'Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection', Electroanalysis, vol. 26, no. 3, pp. 618-624. https://doi.org/10.1002/elan.201300542
Kato D, Oda A, Tanaka M, Iijima S, Kamata T, Todokoro M et al. Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection. Electroanalysis. 2014 Mar;26(3):618-624. https://doi.org/10.1002/elan.201300542
Kato, Dai ; Oda, Atsumu ; Tanaka, Mutsuo ; Iijima, Seiichiro ; Kamata, Tomoyuki ; Todokoro, Masami ; Yoshimi, Yasuo ; Niwa, Osamu. / Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection. In: Electroanalysis. 2014 ; Vol. 26, No. 3. pp. 618-624.
@article{28a7aecf1f594e46b967d4eac8541b16,
title = "Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection",
abstract = "We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.",
keywords = "Electrochemical detection, Lipopolysaccharide, Nanocarbon film electrode, Poly-ε-lysine, Signal amplification",
author = "Dai Kato and Atsumu Oda and Mutsuo Tanaka and Seiichiro Iijima and Tomoyuki Kamata and Masami Todokoro and Yasuo Yoshimi and Osamu Niwa",
year = "2014",
month = "3",
doi = "10.1002/elan.201300542",
language = "English",
volume = "26",
pages = "618--624",
journal = "Electroanalysis",
issn = "1040-0397",
publisher = "Wiley-VCH Verlag",
number = "3",

}

TY - JOUR

T1 - Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

AU - Kato, Dai

AU - Oda, Atsumu

AU - Tanaka, Mutsuo

AU - Iijima, Seiichiro

AU - Kamata, Tomoyuki

AU - Todokoro, Masami

AU - Yoshimi, Yasuo

AU - Niwa, Osamu

PY - 2014/3

Y1 - 2014/3

N2 - We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.

AB - We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.

KW - Electrochemical detection

KW - Lipopolysaccharide

KW - Nanocarbon film electrode

KW - Poly-ε-lysine

KW - Signal amplification

UR - http://www.scopus.com/inward/record.url?scp=84897679836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897679836&partnerID=8YFLogxK

U2 - 10.1002/elan.201300542

DO - 10.1002/elan.201300542

M3 - Article

VL - 26

SP - 618

EP - 624

JO - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

IS - 3

ER -