Abstract
We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.
Original language | English |
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Pages (from-to) | 618-624 |
Number of pages | 7 |
Journal | Electroanalysis |
Volume | 26 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2014 Mar |
Keywords
- Electrochemical detection
- Lipopolysaccharide
- Nanocarbon film electrode
- Poly-ε-lysine
- Signal amplification
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry