Abstract
We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.
| Original language | English |
|---|---|
| Pages (from-to) | 618-624 |
| Number of pages | 7 |
| Journal | Electroanalysis |
| Volume | 26 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2014 Mar |
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Keywords
- Electrochemical detection
- Lipopolysaccharide
- Nanocarbon film electrode
- Poly-ε-lysine
- Signal amplification
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
Cite this
Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection. / Kato, Dai; Oda, Atsumu; Tanaka, Mutsuo; Iijima, Seiichiro; Kamata, Tomoyuki; Todokoro, Masami; Yoshimi, Yasuo; Niwa, Osamu.
In: Electroanalysis, Vol. 26, No. 3, 03.2014, p. 618-624.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection
AU - Kato, Dai
AU - Oda, Atsumu
AU - Tanaka, Mutsuo
AU - Iijima, Seiichiro
AU - Kamata, Tomoyuki
AU - Todokoro, Masami
AU - Yoshimi, Yasuo
AU - Niwa, Osamu
PY - 2014/3
Y1 - 2014/3
N2 - We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.
AB - We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.
KW - Electrochemical detection
KW - Lipopolysaccharide
KW - Nanocarbon film electrode
KW - Poly-ε-lysine
KW - Signal amplification
UR - http://www.scopus.com/inward/record.url?scp=84897679836&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897679836&partnerID=8YFLogxK
U2 - 10.1002/elan.201300542
DO - 10.1002/elan.201300542
M3 - Article
VL - 26
SP - 618
EP - 624
JO - Electroanalysis
JF - Electroanalysis
SN - 1040-0397
IS - 3
ER -