Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

Dai Kato; Atsumu Oda; Mutsuo Tanaka; Seiichiro Iijima; Tomoyuki Kamata; Masami Todokoro; Yasuo Yoshimi; Osamu Niwa

doi:10.1002/elan.201300542

Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

Dai Kato, Atsumu Oda, Mutsuo Tanaka, Seiichiro Iijima, Tomoyuki Kamata, Masami Todokoro, Yasuo Yoshimi, Osamu Niwa

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe²⁺ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.

Original language	English
Pages (from-to)	618-624
Number of pages	7
Journal	Electroanalysis
Volume	26
Issue number	3
DOIs	https://doi.org/10.1002/elan.201300542
Publication status	Published - 2014 Mar 1

Keywords

Electrochemical detection
Lipopolysaccharide
Nanocarbon film electrode
Poly-ε-lysine
Signal amplification

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

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10.1002/elan.201300542

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title = "Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection",

abstract = "We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.",

keywords = "Electrochemical detection, Lipopolysaccharide, Nanocarbon film electrode, Poly-ε-lysine, Signal amplification",

author = "Dai Kato and Atsumu Oda and Mutsuo Tanaka and Seiichiro Iijima and Tomoyuki Kamata and Masami Todokoro and Yasuo Yoshimi and Osamu Niwa",

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language = "English",

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T1 - Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

AU - Kato, Dai

AU - Oda, Atsumu

AU - Tanaka, Mutsuo

AU - Iijima, Seiichiro

AU - Kamata, Tomoyuki

AU - Todokoro, Masami

AU - Yoshimi, Yasuo

AU - Niwa, Osamu

PY - 2014/3/1

Y1 - 2014/3/1

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AB - We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-ε-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.

KW - Electrochemical detection

KW - Lipopolysaccharide

KW - Nanocarbon film electrode

KW - Poly-ε-lysine

KW - Signal amplification

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