Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method

Takashi Masadome, Yasukazu Asano, Toshihiko Imato, Satoshi Ohkubo, Tatsuya Tobita, Hisao Tabei, Yuzuru Iwasaki, Osamu Niwa, Yoshito Fushinuki

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In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalAnalytical and Bioanalytical Chemistry
Issue number4-5
Publication statusPublished - 2002 Jan 1



  • Anti-human albumin
  • Poly (vinyl chloride) film
  • Portable SPR-based chemical sensor
  • Refractive index matching polymer film
  • Surface-plasmon resonance (SPR)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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