TY - JOUR
T1 - Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method
AU - Masadome, Takashi
AU - Asano, Yasukazu
AU - Imato, Toshihiko
AU - Ohkubo, Satoshi
AU - Tobita, Tatsuya
AU - Tabei, Hisao
AU - Iwasaki, Yuzuru
AU - Niwa, Osamu
AU - Fushinuki, Yoshito
PY - 2002/1/1
Y1 - 2002/1/1
N2 - In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.
AB - In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.
KW - Anti-human albumin
KW - Poly (vinyl chloride) film
KW - Portable SPR-based chemical sensor
KW - Refractive index matching polymer film
KW - Surface-plasmon resonance (SPR)
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U2 - 10.1007/s00216-002-1329-4
DO - 10.1007/s00216-002-1329-4
M3 - Article
C2 - 12110971
AN - SCOPUS:0036314770
VL - 373
SP - 222
EP - 226
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 0016-1152
IS - 4-5
ER -