Abstract
In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.
| Original language | English |
|---|---|
| Pages (from-to) | 222-226 |
| Number of pages | 5 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 373 |
| Issue number | 4-5 |
| DOIs | |
| Publication status | Published - 2002 |
| Externally published | Yes |
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Keywords
- Anti-human albumin
- Poly (vinyl chloride) film
- Portable SPR-based chemical sensor
- Refractive index matching polymer film
- Surface-plasmon resonance (SPR)
ASJC Scopus subject areas
- Clinical Biochemistry
- Analytical Chemistry
Cite this
Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method. / Masadome, Takashi; Asano, Yasukazu; Imato, Toshihiko; Ohkubo, Satoshi; Tobita, Tatsuya; Tabei, Hisao; Iwasaki, Yuzuru; Niwa, Osamu; Fushinuki, Yoshito.
In: Analytical and Bioanalytical Chemistry, Vol. 373, No. 4-5, 2002, p. 222-226.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method
AU - Masadome, Takashi
AU - Asano, Yasukazu
AU - Imato, Toshihiko
AU - Ohkubo, Satoshi
AU - Tobita, Tatsuya
AU - Tabei, Hisao
AU - Iwasaki, Yuzuru
AU - Niwa, Osamu
AU - Fushinuki, Yoshito
PY - 2002
Y1 - 2002
N2 - In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.
AB - In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.
KW - Anti-human albumin
KW - Poly (vinyl chloride) film
KW - Portable SPR-based chemical sensor
KW - Refractive index matching polymer film
KW - Surface-plasmon resonance (SPR)
UR - http://www.scopus.com/inward/record.url?scp=0036314770&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036314770&partnerID=8YFLogxK
U2 - 10.1007/s00216-002-1329-4
DO - 10.1007/s00216-002-1329-4
M3 - Article
C2 - 12110971
AN - SCOPUS:0036314770
VL - 373
SP - 222
EP - 226
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 0016-1152
IS - 4-5
ER -