Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method

Takashi Masadome, Yasukazu Asano, Toshihiko Imato, Satoshi Ohkubo, Tatsuya Tobita, Hisao Tabei, Yuzuru Iwasaki, Osamu Niwa, Yoshito Fushinuki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalAnalytical and Bioanalytical Chemistry
Volume373
Issue number4-5
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Surface plasmon resonance
Chemical sensors
Polymer films
Refractive index
Oils
Sensors
Prisms
Tritolyl Phosphates
Diethylhexyl Phthalate
Antigen-antibody reactions
Vinyl Chloride
Glass
Polyvinyl Chloride
Albumins
Casting

Keywords

  • Anti-human albumin
  • Poly (vinyl chloride) film
  • Portable SPR-based chemical sensor
  • Refractive index matching polymer film
  • Surface-plasmon resonance (SPR)

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

Cite this

Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method. / Masadome, Takashi; Asano, Yasukazu; Imato, Toshihiko; Ohkubo, Satoshi; Tobita, Tatsuya; Tabei, Hisao; Iwasaki, Yuzuru; Niwa, Osamu; Fushinuki, Yoshito.

In: Analytical and Bioanalytical Chemistry, Vol. 373, No. 4-5, 2002, p. 222-226.

Research output: Contribution to journalArticle

Masadome, Takashi ; Asano, Yasukazu ; Imato, Toshihiko ; Ohkubo, Satoshi ; Tobita, Tatsuya ; Tabei, Hisao ; Iwasaki, Yuzuru ; Niwa, Osamu ; Fushinuki, Yoshito. / Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method. In: Analytical and Bioanalytical Chemistry. 2002 ; Vol. 373, No. 4-5. pp. 222-226.
@article{aa169c7db2474bb4842688c3be9bc6c0,
title = "Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method",
abstract = "In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.",
keywords = "Anti-human albumin, Poly (vinyl chloride) film, Portable SPR-based chemical sensor, Refractive index matching polymer film, Surface-plasmon resonance (SPR)",
author = "Takashi Masadome and Yasukazu Asano and Toshihiko Imato and Satoshi Ohkubo and Tatsuya Tobita and Hisao Tabei and Yuzuru Iwasaki and Osamu Niwa and Yoshito Fushinuki",
year = "2002",
doi = "10.1007/s00216-002-1329-4",
language = "English",
volume = "373",
pages = "222--226",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "0016-1152",
publisher = "Springer Verlag",
number = "4-5",

}

TY - JOUR

T1 - Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method

AU - Masadome, Takashi

AU - Asano, Yasukazu

AU - Imato, Toshihiko

AU - Ohkubo, Satoshi

AU - Tobita, Tatsuya

AU - Tabei, Hisao

AU - Iwasaki, Yuzuru

AU - Niwa, Osamu

AU - Fushinuki, Yoshito

PY - 2002

Y1 - 2002

N2 - In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.

AB - In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.

KW - Anti-human albumin

KW - Poly (vinyl chloride) film

KW - Portable SPR-based chemical sensor

KW - Refractive index matching polymer film

KW - Surface-plasmon resonance (SPR)

UR - http://www.scopus.com/inward/record.url?scp=0036314770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036314770&partnerID=8YFLogxK

U2 - 10.1007/s00216-002-1329-4

DO - 10.1007/s00216-002-1329-4

M3 - Article

VL - 373

SP - 222

EP - 226

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 0016-1152

IS - 4-5

ER -