Promotion of metal accumulation in nodule of Astragalus sinicus by the expression of the iron-regulated transporter gene in Mesorhizobium huakuii subsp. rengei B3

Akiko Ike, Rutchadaporn Sriprang, Hisayo Ono, Yoshikatsu Murooka, Mitsuo Yamashita

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Toxic metal contamination in agricultural fields is an important worldwide problem. In previous studies, we developed a bioremediation system based on the symbiosis between Astragalus sinicus and the recombinant rhizobium, Mesorhizobium huakuii subsp. rengei B3 developed by overexpressing a synthetic tetrameric metallothionein gene (MTL4) and cDNA encoding the phytochelatin synthase from Arabidopsis thaliana (AtPCS). To promote the transport of metals into the nodules of the rhizobium and the accumulation of metals, the iron-regulated transporter 1 gene from A. thaliana (AtIRT1) was introduced into recombinant strain B3 containing MTL4 or AtPCS in its chromosome. The fused AtIRT1-alkaline phosphatase was expressed in the free-living recombinant rhizobium and the nodule of A. sinicus. The recombinant strain B3 carrying AtIRT1 showed a higher Cd sensitivity and a higher amount of Cd accumulated in free-living culture than the wild-type strain B3. When the recombinant strain B3 established symbiosis with A. sinicus, the introduction of AtIRT1 in the recombinant strain B3 advantaged the accumulation of Cu and As in the nodules of A. sinicus, compared with that of Cd and Zn.

Original languageEnglish
Pages (from-to)642-648
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume105
Issue number6
DOIs
Publication statusPublished - 2008 Jun
Externally publishedYes

Fingerprint

Iron
Genes
Metals
Bioremediation
Metallothionein
Poisons
Phosphatases
Chromosomes
Alkaline Phosphatase
Contamination
Complementary DNA

Keywords

  • heavy metals
  • iron-regulated transporter 1
  • Leguminous plant
  • Rhizobium
  • symbiosis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Promotion of metal accumulation in nodule of Astragalus sinicus by the expression of the iron-regulated transporter gene in Mesorhizobium huakuii subsp. rengei B3. / Ike, Akiko; Sriprang, Rutchadaporn; Ono, Hisayo; Murooka, Yoshikatsu; Yamashita, Mitsuo.

In: Journal of Bioscience and Bioengineering, Vol. 105, No. 6, 06.2008, p. 642-648.

Research output: Contribution to journalArticle

@article{0b52aaf4635645d5b9cd6dd17bc3e194,
title = "Promotion of metal accumulation in nodule of Astragalus sinicus by the expression of the iron-regulated transporter gene in Mesorhizobium huakuii subsp. rengei B3",
abstract = "Toxic metal contamination in agricultural fields is an important worldwide problem. In previous studies, we developed a bioremediation system based on the symbiosis between Astragalus sinicus and the recombinant rhizobium, Mesorhizobium huakuii subsp. rengei B3 developed by overexpressing a synthetic tetrameric metallothionein gene (MTL4) and cDNA encoding the phytochelatin synthase from Arabidopsis thaliana (AtPCS). To promote the transport of metals into the nodules of the rhizobium and the accumulation of metals, the iron-regulated transporter 1 gene from A. thaliana (AtIRT1) was introduced into recombinant strain B3 containing MTL4 or AtPCS in its chromosome. The fused AtIRT1-alkaline phosphatase was expressed in the free-living recombinant rhizobium and the nodule of A. sinicus. The recombinant strain B3 carrying AtIRT1 showed a higher Cd sensitivity and a higher amount of Cd accumulated in free-living culture than the wild-type strain B3. When the recombinant strain B3 established symbiosis with A. sinicus, the introduction of AtIRT1 in the recombinant strain B3 advantaged the accumulation of Cu and As in the nodules of A. sinicus, compared with that of Cd and Zn.",
keywords = "heavy metals, iron-regulated transporter 1, Leguminous plant, Rhizobium, symbiosis",
author = "Akiko Ike and Rutchadaporn Sriprang and Hisayo Ono and Yoshikatsu Murooka and Mitsuo Yamashita",
year = "2008",
month = "6",
doi = "10.1263/jbb.105.642",
language = "English",
volume = "105",
pages = "642--648",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "Elsevier",
number = "6",

}

TY - JOUR

T1 - Promotion of metal accumulation in nodule of Astragalus sinicus by the expression of the iron-regulated transporter gene in Mesorhizobium huakuii subsp. rengei B3

AU - Ike, Akiko

AU - Sriprang, Rutchadaporn

AU - Ono, Hisayo

AU - Murooka, Yoshikatsu

AU - Yamashita, Mitsuo

PY - 2008/6

Y1 - 2008/6

N2 - Toxic metal contamination in agricultural fields is an important worldwide problem. In previous studies, we developed a bioremediation system based on the symbiosis between Astragalus sinicus and the recombinant rhizobium, Mesorhizobium huakuii subsp. rengei B3 developed by overexpressing a synthetic tetrameric metallothionein gene (MTL4) and cDNA encoding the phytochelatin synthase from Arabidopsis thaliana (AtPCS). To promote the transport of metals into the nodules of the rhizobium and the accumulation of metals, the iron-regulated transporter 1 gene from A. thaliana (AtIRT1) was introduced into recombinant strain B3 containing MTL4 or AtPCS in its chromosome. The fused AtIRT1-alkaline phosphatase was expressed in the free-living recombinant rhizobium and the nodule of A. sinicus. The recombinant strain B3 carrying AtIRT1 showed a higher Cd sensitivity and a higher amount of Cd accumulated in free-living culture than the wild-type strain B3. When the recombinant strain B3 established symbiosis with A. sinicus, the introduction of AtIRT1 in the recombinant strain B3 advantaged the accumulation of Cu and As in the nodules of A. sinicus, compared with that of Cd and Zn.

AB - Toxic metal contamination in agricultural fields is an important worldwide problem. In previous studies, we developed a bioremediation system based on the symbiosis between Astragalus sinicus and the recombinant rhizobium, Mesorhizobium huakuii subsp. rengei B3 developed by overexpressing a synthetic tetrameric metallothionein gene (MTL4) and cDNA encoding the phytochelatin synthase from Arabidopsis thaliana (AtPCS). To promote the transport of metals into the nodules of the rhizobium and the accumulation of metals, the iron-regulated transporter 1 gene from A. thaliana (AtIRT1) was introduced into recombinant strain B3 containing MTL4 or AtPCS in its chromosome. The fused AtIRT1-alkaline phosphatase was expressed in the free-living recombinant rhizobium and the nodule of A. sinicus. The recombinant strain B3 carrying AtIRT1 showed a higher Cd sensitivity and a higher amount of Cd accumulated in free-living culture than the wild-type strain B3. When the recombinant strain B3 established symbiosis with A. sinicus, the introduction of AtIRT1 in the recombinant strain B3 advantaged the accumulation of Cu and As in the nodules of A. sinicus, compared with that of Cd and Zn.

KW - heavy metals

KW - iron-regulated transporter 1

KW - Leguminous plant

KW - Rhizobium

KW - symbiosis

UR - http://www.scopus.com/inward/record.url?scp=48949118105&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48949118105&partnerID=8YFLogxK

U2 - 10.1263/jbb.105.642

DO - 10.1263/jbb.105.642

M3 - Article

C2 - 18640604

AN - SCOPUS:48949118105

VL - 105

SP - 642

EP - 648

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 6

ER -