Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen

Kayo Ohkouchi, Seiji Kawamoto, Kenji Tatsugawa, Noboru Yoshikawa, Yuki Takaoka, Sayumi Miyauchi, Tsunehiro Aki, Mitsuo Yamashita, Yoshikatsu Murooka, Kazuhisa Ono

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Lactic acid bacteria (LAB) represent an attractive delivery vehicle for oral allergy vaccine because of their safety as a food microorganism as well as their potent adjuvant activity triggering anti-allergic immune response. Here, we report the generation of recombinant LAB expressing a major Japanese cedar pollen allergen Cry j 1 (Cry j 1-LAB), and their prophylactic effect in vivo. To facilitate heterologous expression, the codon usage in the Cry j 1 gene was optimized for the host LAB strain Lactobacillus plantarum by the recursive PCR-based exhaustive site-directed mutagenesis. Use of the codon-optimized Cry j 1 cDNA and a lactate dehydrogenase gene fusion system led to a successful production of recombinant Cry j 1 in L. plantarum NCL21. We also found that oral vaccination with the Cry j 1-LAB suppressed allergen-specific IgE response and nasal symptoms in a murine model of cedar pollinosis.

Original languageEnglish
Pages (from-to)536-541
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume113
Issue number4
DOIs
Publication statusPublished - 2012 Apr

Fingerprint

Allergens
Vaccines
Lactic acid
Lactic Acid
Bacteria
Genes
Allergies
Anti-Allergic Agents
Mutagenesis
L-Lactate Dehydrogenase
Microorganisms
Immunoglobulin E
Fusion reactions
Complementary DNA

Keywords

  • Allergen
  • Codon optimization
  • IgE
  • Japanese cedar pollen
  • Lactobacillus

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Ohkouchi, K., Kawamoto, S., Tatsugawa, K., Yoshikawa, N., Takaoka, Y., Miyauchi, S., ... Ono, K. (2012). Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen. Journal of Bioscience and Bioengineering, 113(4), 536-541. https://doi.org/10.1016/j.jbiosc.2011.11.025

Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen. / Ohkouchi, Kayo; Kawamoto, Seiji; Tatsugawa, Kenji; Yoshikawa, Noboru; Takaoka, Yuki; Miyauchi, Sayumi; Aki, Tsunehiro; Yamashita, Mitsuo; Murooka, Yoshikatsu; Ono, Kazuhisa.

In: Journal of Bioscience and Bioengineering, Vol. 113, No. 4, 04.2012, p. 536-541.

Research output: Contribution to journalArticle

Ohkouchi, K, Kawamoto, S, Tatsugawa, K, Yoshikawa, N, Takaoka, Y, Miyauchi, S, Aki, T, Yamashita, M, Murooka, Y & Ono, K 2012, 'Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen', Journal of Bioscience and Bioengineering, vol. 113, no. 4, pp. 536-541. https://doi.org/10.1016/j.jbiosc.2011.11.025
Ohkouchi, Kayo ; Kawamoto, Seiji ; Tatsugawa, Kenji ; Yoshikawa, Noboru ; Takaoka, Yuki ; Miyauchi, Sayumi ; Aki, Tsunehiro ; Yamashita, Mitsuo ; Murooka, Yoshikatsu ; Ono, Kazuhisa. / Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen. In: Journal of Bioscience and Bioengineering. 2012 ; Vol. 113, No. 4. pp. 536-541.
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