Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen

Kayo Ohkouchi, Seiji Kawamoto, Kenji Tatsugawa, Noboru Yoshikawa, Yuki Takaoka, Sayumi Miyauchi, Tsunehiro Aki, Mitsuo Yamashita, Yoshikatsu Murooka, Kazuhisa Ono

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Lactic acid bacteria (LAB) represent an attractive delivery vehicle for oral allergy vaccine because of their safety as a food microorganism as well as their potent adjuvant activity triggering anti-allergic immune response. Here, we report the generation of recombinant LAB expressing a major Japanese cedar pollen allergen Cry j 1 (Cry j 1-LAB), and their prophylactic effect in vivo. To facilitate heterologous expression, the codon usage in the Cry j 1 gene was optimized for the host LAB strain Lactobacillus plantarum by the recursive PCR-based exhaustive site-directed mutagenesis. Use of the codon-optimized Cry j 1 cDNA and a lactate dehydrogenase gene fusion system led to a successful production of recombinant Cry j 1 in L. plantarum NCL21. We also found that oral vaccination with the Cry j 1-LAB suppressed allergen-specific IgE response and nasal symptoms in a murine model of cedar pollinosis.

Original languageEnglish
Pages (from-to)536-541
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume113
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1

Keywords

  • Allergen
  • Codon optimization
  • IgE
  • Japanese cedar pollen
  • Lactobacillus

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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