Protein crystallization induced by elecrically driven bubble knife

H. Kuriki, S. Takasawa, S. Sakuma, K. Shinmura, G. Kurisu, F. Arai, Y. Yamanishi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have succeeded in protein crystallization by electrically-driven mono-dispersed micro-bubble knife. The dispensed directional micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-driven mono-dispersed micro-bubble was much higher than that of control condition. It was confirmed that induction force of protein crystallization by electrically-driven mono-dispersed micro-bubble increased with the amplification of input power of electrical circuit of electrically-induced micro-bubble knife.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1317-1319
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Keywords

  • Micro-bubble
  • Protein crystallization

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Kuriki, H., Takasawa, S., Sakuma, S., Shinmura, K., Kurisu, G., Arai, F., & Yamanishi, Y. (2013). Protein crystallization induced by elecrically driven bubble knife. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1317-1319). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.