Prototyping the experimental setup to quantify the tissue oxygen consumption rate and its preliminary test

Nobuo Watanabe, Masahiro Shibata, S. Sawada, K. Mizukami

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In order to establish a reliable and practical method to make a diagnosis on the viability of an amputated extremity, we propose a method to evaluate the oxygen consumption rate. To validate this concept, we prototyped an experimental system with which the oxygen transfer rate into tissue can be assessed by the rate of change of the decrease in dissolved oxygen (DO) concentration within the buffer fluid surrounding the target tissue. The purpose of this study is to examine the feasibility of our prototyped experimental system by comparison between fresh and non-fresh rat skeletal muscles. The results show that the fresher tissue transferred more oxygen to the tissue, which suggests that tissue oxygen consumption is highly related to tissue freshness and can indirectly assess the tissue viability.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages247-255
Number of pages9
Volume876
ISBN (Print)9781493930227
DOIs
Publication statusPublished - 2016
Event42nd Annual Meeting of International Society on Oxygen Transport to Tissue - London, United Kingdom
Duration: 2014 Jun 282014 Jul 3

Publication series

NameAdvances in Experimental Medicine and Biology
Volume876
ISSN (Print)00652598
ISSN (Electronic)22148019

Other

Other42nd Annual Meeting of International Society on Oxygen Transport to Tissue
CountryUnited Kingdom
CityLondon
Period14/6/2814/7/3

Keywords

  • Dissolved oxygen
  • Oxygendiffusion
  • Temperature
  • Tissue freshness
  • Tissue viability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Fingerprint Dive into the research topics of 'Prototyping the experimental setup to quantify the tissue oxygen consumption rate and its preliminary test'. Together they form a unique fingerprint.

  • Cite this

    Watanabe, N., Shibata, M., Sawada, S., & Mizukami, K. (2016). Prototyping the experimental setup to quantify the tissue oxygen consumption rate and its preliminary test. In Advances in Experimental Medicine and Biology (Vol. 876, pp. 247-255). (Advances in Experimental Medicine and Biology; Vol. 876). Springer New York LLC. https://doi.org/10.1007/978-1-4939-3023-4_31