Quantification of fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry

Maya Kamao, Naoko Tsugawa, Yoshitomo Suhara, Akimori Wada, Toshiyuki Mori, Kazuo Murata, Riichiro Nishino, Tetsuya Ukita, Kazuhiro Uenishi, Kiyoshi Tanaka, Toshio Okano

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Sensitive quantification method for fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry was developed. Vitamins A, D and E were extracted from 10.0 mL of breast milk after saponifying by basic condition. Vitamin K derivatives were extracted from 3.0 mL of breast milk after lipase treatment. The corresponding stable isotope-labeled compounds were used as internal standards. For the determination of vitamin D compounds, derivatization with a Cookson-type reagent was performed. All fat-soluble vitamins were determined by liquid chromatography-tandem mass spectrometry in the positive ion mode. The detection limits of all analytes were 1-250 pg per 50 μL. The recoveries of fat-soluble vitamins were 91-105%. Inter-assay CV values of each vitamin were 1.9-11.9%. The mean concentrations of retinol, vitamin D3, 25-hydroxyvitamin D3, α-tocopherol, phylloquinone and menaquinone-4 were 0.455 μg/mL, 0.088 ng/mL, 0.081 ng/mL, 5.087 μg/mL, 3.771 ng/mL, and 1.795 ng/mL, respectively (n = 82). This method makes possible to determine fat-soluble vitamins with a wide range of polarities in human breast milk. The assay may be useful for large-scale studies.

Original languageEnglish
Pages (from-to)192-200
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume859
Issue number2
DOIs
Publication statusPublished - 2007 Nov 15
Externally publishedYes

Fingerprint

Liquid chromatography
Vitamins
Mass spectrometry
Fats
Vitamin A
Assays
Vitamin K 1
Calcifediol
Tocopherols
Vitamin K
Cholecalciferol
Lipase
Vitamin D
Isotopes
Positive ions
Human Milk
Derivatives
Recovery

Keywords

  • Breast milk
  • Fat-soluble vitamins
  • Liquid chromatography-tandem mass spectrometry
  • Vitamin A
  • Vitamin D
  • Vitamin E
  • Vitamin K

ASJC Scopus subject areas

  • Biochemistry

Cite this

Quantification of fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry. / Kamao, Maya; Tsugawa, Naoko; Suhara, Yoshitomo; Wada, Akimori; Mori, Toshiyuki; Murata, Kazuo; Nishino, Riichiro; Ukita, Tetsuya; Uenishi, Kazuhiro; Tanaka, Kiyoshi; Okano, Toshio.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 859, No. 2, 15.11.2007, p. 192-200.

Research output: Contribution to journalArticle

Kamao, Maya ; Tsugawa, Naoko ; Suhara, Yoshitomo ; Wada, Akimori ; Mori, Toshiyuki ; Murata, Kazuo ; Nishino, Riichiro ; Ukita, Tetsuya ; Uenishi, Kazuhiro ; Tanaka, Kiyoshi ; Okano, Toshio. / Quantification of fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2007 ; Vol. 859, No. 2. pp. 192-200.
@article{f9761c9022ad469b9ffc7efcc9c3579b,
title = "Quantification of fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry",
abstract = "Sensitive quantification method for fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry was developed. Vitamins A, D and E were extracted from 10.0 mL of breast milk after saponifying by basic condition. Vitamin K derivatives were extracted from 3.0 mL of breast milk after lipase treatment. The corresponding stable isotope-labeled compounds were used as internal standards. For the determination of vitamin D compounds, derivatization with a Cookson-type reagent was performed. All fat-soluble vitamins were determined by liquid chromatography-tandem mass spectrometry in the positive ion mode. The detection limits of all analytes were 1-250 pg per 50 μL. The recoveries of fat-soluble vitamins were 91-105{\%}. Inter-assay CV values of each vitamin were 1.9-11.9{\%}. The mean concentrations of retinol, vitamin D3, 25-hydroxyvitamin D3, α-tocopherol, phylloquinone and menaquinone-4 were 0.455 μg/mL, 0.088 ng/mL, 0.081 ng/mL, 5.087 μg/mL, 3.771 ng/mL, and 1.795 ng/mL, respectively (n = 82). This method makes possible to determine fat-soluble vitamins with a wide range of polarities in human breast milk. The assay may be useful for large-scale studies.",
keywords = "Breast milk, Fat-soluble vitamins, Liquid chromatography-tandem mass spectrometry, Vitamin A, Vitamin D, Vitamin E, Vitamin K",
author = "Maya Kamao and Naoko Tsugawa and Yoshitomo Suhara and Akimori Wada and Toshiyuki Mori and Kazuo Murata and Riichiro Nishino and Tetsuya Ukita and Kazuhiro Uenishi and Kiyoshi Tanaka and Toshio Okano",
year = "2007",
month = "11",
day = "15",
doi = "10.1016/j.jchromb.2007.09.023",
language = "English",
volume = "859",
pages = "192--200",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Quantification of fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry

AU - Kamao, Maya

AU - Tsugawa, Naoko

AU - Suhara, Yoshitomo

AU - Wada, Akimori

AU - Mori, Toshiyuki

AU - Murata, Kazuo

AU - Nishino, Riichiro

AU - Ukita, Tetsuya

AU - Uenishi, Kazuhiro

AU - Tanaka, Kiyoshi

AU - Okano, Toshio

PY - 2007/11/15

Y1 - 2007/11/15

N2 - Sensitive quantification method for fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry was developed. Vitamins A, D and E were extracted from 10.0 mL of breast milk after saponifying by basic condition. Vitamin K derivatives were extracted from 3.0 mL of breast milk after lipase treatment. The corresponding stable isotope-labeled compounds were used as internal standards. For the determination of vitamin D compounds, derivatization with a Cookson-type reagent was performed. All fat-soluble vitamins were determined by liquid chromatography-tandem mass spectrometry in the positive ion mode. The detection limits of all analytes were 1-250 pg per 50 μL. The recoveries of fat-soluble vitamins were 91-105%. Inter-assay CV values of each vitamin were 1.9-11.9%. The mean concentrations of retinol, vitamin D3, 25-hydroxyvitamin D3, α-tocopherol, phylloquinone and menaquinone-4 were 0.455 μg/mL, 0.088 ng/mL, 0.081 ng/mL, 5.087 μg/mL, 3.771 ng/mL, and 1.795 ng/mL, respectively (n = 82). This method makes possible to determine fat-soluble vitamins with a wide range of polarities in human breast milk. The assay may be useful for large-scale studies.

AB - Sensitive quantification method for fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry was developed. Vitamins A, D and E were extracted from 10.0 mL of breast milk after saponifying by basic condition. Vitamin K derivatives were extracted from 3.0 mL of breast milk after lipase treatment. The corresponding stable isotope-labeled compounds were used as internal standards. For the determination of vitamin D compounds, derivatization with a Cookson-type reagent was performed. All fat-soluble vitamins were determined by liquid chromatography-tandem mass spectrometry in the positive ion mode. The detection limits of all analytes were 1-250 pg per 50 μL. The recoveries of fat-soluble vitamins were 91-105%. Inter-assay CV values of each vitamin were 1.9-11.9%. The mean concentrations of retinol, vitamin D3, 25-hydroxyvitamin D3, α-tocopherol, phylloquinone and menaquinone-4 were 0.455 μg/mL, 0.088 ng/mL, 0.081 ng/mL, 5.087 μg/mL, 3.771 ng/mL, and 1.795 ng/mL, respectively (n = 82). This method makes possible to determine fat-soluble vitamins with a wide range of polarities in human breast milk. The assay may be useful for large-scale studies.

KW - Breast milk

KW - Fat-soluble vitamins

KW - Liquid chromatography-tandem mass spectrometry

KW - Vitamin A

KW - Vitamin D

KW - Vitamin E

KW - Vitamin K

UR - http://www.scopus.com/inward/record.url?scp=35748943524&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35748943524&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2007.09.023

DO - 10.1016/j.jchromb.2007.09.023

M3 - Article

C2 - 17942378

AN - SCOPUS:35748943524

VL - 859

SP - 192

EP - 200

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 2

ER -