Rapid and repetitive immunoassay with a surface acoustic wave device for monitoring of dust mite allergens

Koji Toma, Mai Horibe, Chisato Kishikawa, Naoyuki Yoshimura, Takahiro Arakawa, Hiromi Yatsuda, Hiroji Shimomura, Kohji Mitsubayashi

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

In this work, we investigated a new approach to shorten a measurement time of house dust mite (HDM) allergens with a surface acoustic wave (SAW) immunosensor for improvement of continuity in monitoring of airborne HDM. It was achieved by employing differential method with which a slope of phase change was used as a sensor output instead of a phase shift that was used conventionally. Compared to the conventional method, measurement time of Group 1 HDM allergens of Dermatophagoides farinae (Der f 1) was shortened by 10 min from 30 min, while a sensitivity of SAW immunosensor was comparable (limit of detection of 20.1 ng/mL). 300 ng/mL Der f 1 was repeatedly measured ten times by the differential method, and it resulted in highly reproduced sensor output as represented by a coefficient of variation of 5.7%. In addition, due to smaller amount of bound detection antibodies on the sensor surface, decline of the baseline during the semi-continuous measurement that happened in the conventional method was not observed. Selectivity of the differential method was also investigated with four different common environmental allergens including Der f 1. No significant output was observed from the samples without Der f 1, and a high selectivity of the differential method with SAW immunosensor was validated. These results suggest that use of the differential method with SAW immunosensor would lead to advanced semi-continuous measurement with high continuity of airborne HDM allergens when it is incorporated with a monitoring system.

Original languageEnglish
Pages (from-to)924-929
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume248
DOIs
Publication statusPublished - 2017
Externally publishedYes

Keywords

  • House dust mite allergen
  • Immunosensor
  • Rapid
  • Repetitive
  • Surface acoustic wave

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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