Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel

Akira Yamaguchi; Peng Jin; Hiroshi Tsuchiyama; Takao Masuda; Kai Sun; Shigeki Matsuo; Hiroaki Misawa

doi:10.1016/S0003-2670(02)00634-7

Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel

Akira Yamaguchi, Peng Jin, Hiroshi Tsuchiyama, Takao Masuda, Kai Sun, Shigeki Matsuo, Hiroaki Misawa

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.

Original language	English
Pages (from-to)	143-152
Number of pages	10
Journal	Analytica Chimica Acta
Volume	468
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2670(02)00634-7
Publication status	Published - 2002 Sep 10
Externally published	Yes

Keywords

Enzyme sensor
Microchannel
Microelectrode
PDMS

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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title = "Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel",

abstract = "Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.",

keywords = "Enzyme sensor, Microchannel, Microelectrode, PDMS",

author = "Akira Yamaguchi and Peng Jin and Hiroshi Tsuchiyama and Takao Masuda and Kai Sun and Shigeki Matsuo and Hiroaki Misawa",

note = "Funding Information: The authors acknowledge a Grant-in-Aid for Scientific Research (No. 10450100) and a grant for the Collaboration between University and Society (No. 11793004) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. The work was also supported partly by New Energy Industrial Technology Development Organization (NEDO) regional consortium project “Development of Ultrasensitive Micro-Integrated Analysis Systems”, and the Satellite Venture Business Laboratory of the University of Tokushima. ",

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AU - Yamaguchi, Akira

AU - Jin, Peng

AU - Tsuchiyama, Hiroshi

AU - Masuda, Takao

AU - Sun, Kai

AU - Matsuo, Shigeki

AU - Misawa, Hiroaki

N1 - Funding Information: The authors acknowledge a Grant-in-Aid for Scientific Research (No. 10450100) and a grant for the Collaboration between University and Society (No. 11793004) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. The work was also supported partly by New Energy Industrial Technology Development Organization (NEDO) regional consortium project “Development of Ultrasensitive Micro-Integrated Analysis Systems”, and the Satellite Venture Business Laboratory of the University of Tokushima.

PY - 2002/9/10

Y1 - 2002/9/10

N2 - Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.

AB - Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.

KW - Enzyme sensor

KW - Microchannel

KW - Microelectrode

KW - PDMS

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