Abstract
Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.
Original language | English |
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Pages (from-to) | 143-152 |
Number of pages | 10 |
Journal | Analytica Chimica Acta |
Volume | 468 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2002 Sep 10 |
Externally published | Yes |
Keywords
- Enzyme sensor
- Microchannel
- Microelectrode
- PDMS
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy