Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel

Akira Yamaguchi, Peng Jin, Hiroshi Tsuchiyama, Takao Masuda, Kai Sun, Shigeki Matsuo, Hiroaki Misawa

Research output: Contribution to journalArticle

41 Citations (Scopus)


Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalAnalytica Chimica Acta
Issue number1
Publication statusPublished - 2002 Sep 10
Externally publishedYes



  • Enzyme sensor
  • Microchannel
  • Microelectrode
  • PDMS

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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