Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel

Akira Yamaguchi, Peng Jin, Hiroshi Tsuchiyama, Takao Masuda, Kai Sun, Shigeki Matsuo, Hiroaki Misawa

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalAnalytica Chimica Acta
Volume468
Issue number1
DOIs
Publication statusPublished - 2002 Sep 10
Externally publishedYes

Fingerprint

Enzyme sensors
Electrochemical sensors
Microfluidics
Microelectrodes
enzyme
sensor
Fabrication
Sensors
Substrates
substrate
Leakage (fluid)
Plastics
Glass
leakage
Enzymes
glass
plastic
baysilon

Keywords

  • Enzyme sensor
  • Microchannel
  • Microelectrode
  • PDMS

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel. / Yamaguchi, Akira; Jin, Peng; Tsuchiyama, Hiroshi; Masuda, Takao; Sun, Kai; Matsuo, Shigeki; Misawa, Hiroaki.

In: Analytica Chimica Acta, Vol. 468, No. 1, 10.09.2002, p. 143-152.

Research output: Contribution to journalArticle

Yamaguchi, Akira ; Jin, Peng ; Tsuchiyama, Hiroshi ; Masuda, Takao ; Sun, Kai ; Matsuo, Shigeki ; Misawa, Hiroaki. / Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel. In: Analytica Chimica Acta. 2002 ; Vol. 468, No. 1. pp. 143-152.
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