Abstract
Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.
| Original language | English |
|---|---|
| Pages (from-to) | 143-152 |
| Number of pages | 10 |
| Journal | Analytica Chimica Acta |
| Volume | 468 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2002 Sep 10 |
| Externally published | Yes |
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Keywords
- Enzyme sensor
- Microchannel
- Microelectrode
- PDMS
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel. / Yamaguchi, Akira; Jin, Peng; Tsuchiyama, Hiroshi; Masuda, Takao; Sun, Kai; Matsuo, Shigeki; Misawa, Hiroaki.
In: Analytica Chimica Acta, Vol. 468, No. 1, 10.09.2002, p. 143-152.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel
AU - Yamaguchi, Akira
AU - Jin, Peng
AU - Tsuchiyama, Hiroshi
AU - Masuda, Takao
AU - Sun, Kai
AU - Matsuo, Shigeki
AU - Misawa, Hiroaki
PY - 2002/9/10
Y1 - 2002/9/10
N2 - Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.
AB - Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.
KW - Enzyme sensor
KW - Microchannel
KW - Microelectrode
KW - PDMS
UR - http://www.scopus.com/inward/record.url?scp=0037057110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037057110&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(02)00634-7
DO - 10.1016/S0003-2670(02)00634-7
M3 - Article
VL - 468
SP - 143
EP - 152
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1
ER -