Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode

Takashi K. Saito, Mareyuki Takahashi, Hitoshi Muguruma, Takafumi Suzuki, Naoto Kakuta, Etsuo Niki, Kunihiko Mabuchi

Research output: Contribution to journalConference article

Abstract

We report a new aspect of rapid (< 30 seconds) light-induced cell membrane damage photosensitized by 5,5″-bis(aminomethyl)-2,2′:5′,2″-terthiophene dihydrochloride (BAT), which is a water-soluble α-terthienyl analogue and a monomer of conducting polymer, using a high-power laser (light intensity 1.6 W cm-2). A microelectrode insertion process to cell could be improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to insertion. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Three toxic processes of the cells by BAT can be identified: the first is a non-light-mediated toxicity dependent on BAT exposure time; the second is a phototoxicity independent of BAT exposure time when the BAT concentration is 2 - 10 μM range; and the third is a phototoxicity dependent on BAT exposure time with 20 μM of BAT concentration in case that cytotoxicity decreases when α-tocopherol, an anti-oxidant, is added to a cell membrane. This is the typical phototoxicity that the phospholipid peroxidation chain reaction triggered by a reactive oxygen species generated by a triplet state of BAT.

Original languageEnglish
Pages (from-to)2953-2956
Number of pages4
JournalAnnual Reports of the Research Reactor Institute, Kyoto University
Volume3
Publication statusPublished - 2001 Dec 1
Event23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Istanbul, Turkey
Duration: 2001 Oct 252001 Oct 28

Keywords

  • Membrane
  • Microelectrode
  • PC12
  • Photosensitizer

ASJC Scopus subject areas

  • Energy Engineering and Power Technology
  • Mechanical Engineering

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