Ratio of surface roughness to flow scale as additional parameter for shear-induced hemolysis

Nobuo Watanabe, Sunao Ueda, Kentaro Nagashima, Taku Oguri, Toshihiro Mita

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: In addition to the conventional knowledge that shear stress and its exposure time should have a large impact on hemolysis, it became obvious through Dr. Maruyama’s study that surface roughness would be the additional factor for high shear-induced hemolysis. Concerning this new information, we hypothesized that the ratio of surface roughness to the flow scale should play a role as the additional factor for shear-induced hemolysis. The purpose of this study was to develop a constant shear generator as the method to provide a controlled shear flow field with the combination between the controlled surface roughness and the flow scale to the blood cells. Its preliminary application was to validate our hypothesis. Methods: We prototyped the constant shear stress generator with the cylindrical cone-cup structure made from the acrylic material. This chamber had 3 flow scales of 1.00, 1.25, and 1.5 mm according to the change of the inner stationary cone, at which the surface roughness was distributed into the several levels between 0.14 and 0.92 micrometers in arithmetic average roughness. Using this shear chamber, we examined what effect the flow scale and the surface roughness had on hemolysis. Results: Our experimental data showed the tendency of a positive correlation between the ratio of surface roughness to the flow scale and the induced hemolysis levels, validating our hypothesis. Conclusions: The ratio of the surface roughness to the flow scale should be the additional parameter for shearinduced hemolysis.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalInternational Journal of Artificial Organs
Volume39
Issue number4
DOIs
Publication statusPublished - 2016 Apr 1

Fingerprint

Surface roughness
Cones
Shear stress
Shear flow
Acrylics
Flow fields
Blood
Cells

Keywords

  • Flow scale
  • Shear-induced hemolysis
  • Surface roughness

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

Ratio of surface roughness to flow scale as additional parameter for shear-induced hemolysis. / Watanabe, Nobuo; Ueda, Sunao; Nagashima, Kentaro; Oguri, Taku; Mita, Toshihiro.

In: International Journal of Artificial Organs, Vol. 39, No. 4, 01.04.2016, p. 205-210.

Research output: Contribution to journalArticle

Watanabe, Nobuo ; Ueda, Sunao ; Nagashima, Kentaro ; Oguri, Taku ; Mita, Toshihiro. / Ratio of surface roughness to flow scale as additional parameter for shear-induced hemolysis. In: International Journal of Artificial Organs. 2016 ; Vol. 39, No. 4. pp. 205-210.
@article{da3b0e988da84daa923b6526ce1d566b,
title = "Ratio of surface roughness to flow scale as additional parameter for shear-induced hemolysis",
abstract = "Background: In addition to the conventional knowledge that shear stress and its exposure time should have a large impact on hemolysis, it became obvious through Dr. Maruyama’s study that surface roughness would be the additional factor for high shear-induced hemolysis. Concerning this new information, we hypothesized that the ratio of surface roughness to the flow scale should play a role as the additional factor for shear-induced hemolysis. The purpose of this study was to develop a constant shear generator as the method to provide a controlled shear flow field with the combination between the controlled surface roughness and the flow scale to the blood cells. Its preliminary application was to validate our hypothesis. Methods: We prototyped the constant shear stress generator with the cylindrical cone-cup structure made from the acrylic material. This chamber had 3 flow scales of 1.00, 1.25, and 1.5 mm according to the change of the inner stationary cone, at which the surface roughness was distributed into the several levels between 0.14 and 0.92 micrometers in arithmetic average roughness. Using this shear chamber, we examined what effect the flow scale and the surface roughness had on hemolysis. Results: Our experimental data showed the tendency of a positive correlation between the ratio of surface roughness to the flow scale and the induced hemolysis levels, validating our hypothesis. Conclusions: The ratio of the surface roughness to the flow scale should be the additional parameter for shearinduced hemolysis.",
keywords = "Flow scale, Shear-induced hemolysis, Surface roughness",
author = "Nobuo Watanabe and Sunao Ueda and Kentaro Nagashima and Taku Oguri and Toshihiro Mita",
year = "2016",
month = "4",
day = "1",
doi = "10.5301/ijao.5000500",
language = "English",
volume = "39",
pages = "205--210",
journal = "International Journal of Artificial Organs",
issn = "0391-3988",
publisher = "Wichtig Publishing",
number = "4",

}

TY - JOUR

T1 - Ratio of surface roughness to flow scale as additional parameter for shear-induced hemolysis

AU - Watanabe, Nobuo

AU - Ueda, Sunao

AU - Nagashima, Kentaro

AU - Oguri, Taku

AU - Mita, Toshihiro

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Background: In addition to the conventional knowledge that shear stress and its exposure time should have a large impact on hemolysis, it became obvious through Dr. Maruyama’s study that surface roughness would be the additional factor for high shear-induced hemolysis. Concerning this new information, we hypothesized that the ratio of surface roughness to the flow scale should play a role as the additional factor for shear-induced hemolysis. The purpose of this study was to develop a constant shear generator as the method to provide a controlled shear flow field with the combination between the controlled surface roughness and the flow scale to the blood cells. Its preliminary application was to validate our hypothesis. Methods: We prototyped the constant shear stress generator with the cylindrical cone-cup structure made from the acrylic material. This chamber had 3 flow scales of 1.00, 1.25, and 1.5 mm according to the change of the inner stationary cone, at which the surface roughness was distributed into the several levels between 0.14 and 0.92 micrometers in arithmetic average roughness. Using this shear chamber, we examined what effect the flow scale and the surface roughness had on hemolysis. Results: Our experimental data showed the tendency of a positive correlation between the ratio of surface roughness to the flow scale and the induced hemolysis levels, validating our hypothesis. Conclusions: The ratio of the surface roughness to the flow scale should be the additional parameter for shearinduced hemolysis.

AB - Background: In addition to the conventional knowledge that shear stress and its exposure time should have a large impact on hemolysis, it became obvious through Dr. Maruyama’s study that surface roughness would be the additional factor for high shear-induced hemolysis. Concerning this new information, we hypothesized that the ratio of surface roughness to the flow scale should play a role as the additional factor for shear-induced hemolysis. The purpose of this study was to develop a constant shear generator as the method to provide a controlled shear flow field with the combination between the controlled surface roughness and the flow scale to the blood cells. Its preliminary application was to validate our hypothesis. Methods: We prototyped the constant shear stress generator with the cylindrical cone-cup structure made from the acrylic material. This chamber had 3 flow scales of 1.00, 1.25, and 1.5 mm according to the change of the inner stationary cone, at which the surface roughness was distributed into the several levels between 0.14 and 0.92 micrometers in arithmetic average roughness. Using this shear chamber, we examined what effect the flow scale and the surface roughness had on hemolysis. Results: Our experimental data showed the tendency of a positive correlation between the ratio of surface roughness to the flow scale and the induced hemolysis levels, validating our hypothesis. Conclusions: The ratio of the surface roughness to the flow scale should be the additional parameter for shearinduced hemolysis.

KW - Flow scale

KW - Shear-induced hemolysis

KW - Surface roughness

UR - http://www.scopus.com/inward/record.url?scp=84976382015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976382015&partnerID=8YFLogxK

U2 - 10.5301/ijao.5000500

DO - 10.5301/ijao.5000500

M3 - Article

VL - 39

SP - 205

EP - 210

JO - International Journal of Artificial Organs

JF - International Journal of Artificial Organs

SN - 0391-3988

IS - 4

ER -