Reconfigurable microfluidic device with discretized sidewall

Masahiro Oono, Keisuke Yamaguchi, Amirul Rasyid, Atsushi Takano, Masato Tanaka, Nobuyuki Futai

Research output: Contribution to journalArticle

  • 1 Citations

Abstract

Various microfluidic features, such as traps, have been used to manipulate flows, cells, and other particles within microfluidic systems. However, these features often become undesirable in subsequent steps requiring different fluidic configurations. To meet the changing needs of various microfluidic configurations, we developed a reconfigurable microfluidic channel with movable sidewalls using mechanically discretized sidewalls of laterally aligned rectangular pins. The user can deform the channel sidewall at any time after fabrication by sliding the pins. We confirmed that the flow resistance of the straight microchannel could be reversibly adjusted in the range of 101-105Pa s/μl by manually displacing one of the pins comprising the microchannel sidewall. The reconfigurable microchannel also made it possible to manipulate flows and cells by creating a segmented patterned culture of COS-7 cells and a coculture of human umbilical vein endothelial cells (HUVECs) and human lung fibroblasts (hLFs) inside the microchannel. The reconfigurable microfluidic device successfully maintained a culture of COS-7 cells in a log phase throughout the entire period of 216 h. Furthermore, we performed a migration assay of cocultured HUVEC and hLF spheroids within one microchannel and observed their migration toward each other.

LanguageEnglish
Article number034103
JournalBiomicrofluidics
Volume11
Issue number3
DOIs
StatePublished - 2017 May 1

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microfluidic devices
microchannels
Microchannels
Microfluidics
Endothelial cells
cells
veins
flow resistance
fluidics
spheroids
fibroblasts
Fluidics
Fibroblasts
configurations
lungs
sliding
Assays
traps
Fabrication
fabrication

ASJC Scopus subject areas

  • Materials Science(all)
  • Molecular Biology
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Oono, M., Yamaguchi, K., Rasyid, A., Takano, A., Tanaka, M., & Futai, N. (2017). Reconfigurable microfluidic device with discretized sidewall. Biomicrofluidics, 11(3), [034103]. DOI: 10.1063/1.4983148

Reconfigurable microfluidic device with discretized sidewall. / Oono, Masahiro; Yamaguchi, Keisuke; Rasyid, Amirul; Takano, Atsushi; Tanaka, Masato; Futai, Nobuyuki.

In: Biomicrofluidics, Vol. 11, No. 3, 034103, 01.05.2017.

Research output: Contribution to journalArticle

Oono, M, Yamaguchi, K, Rasyid, A, Takano, A, Tanaka, M & Futai, N 2017, 'Reconfigurable microfluidic device with discretized sidewall' Biomicrofluidics, vol. 11, no. 3, 034103. DOI: 10.1063/1.4983148
Oono M, Yamaguchi K, Rasyid A, Takano A, Tanaka M, Futai N. Reconfigurable microfluidic device with discretized sidewall. Biomicrofluidics. 2017 May 1;11(3). 034103. Available from, DOI: 10.1063/1.4983148
Oono, Masahiro ; Yamaguchi, Keisuke ; Rasyid, Amirul ; Takano, Atsushi ; Tanaka, Masato ; Futai, Nobuyuki. / Reconfigurable microfluidic device with discretized sidewall. In: Biomicrofluidics. 2017 ; Vol. 11, No. 3.
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