Redox reactions of cytochrome c on self-assembled monolayers of mercaptobenzoic acid and mercaptohydrocinnamic acid adsorbed on Au (111) electrodes

Shinichiro Imabayashi, Takahiro Mita, Zhi Qiang Feng, Minehiko Iida, Katsumi Niki, Takashi Kakiuchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The redox reactions of cytochrome c (cyt c) adsorbed on self-assembled monolayers (SAMs) of 4-mercaptobenzoic acid (4-MBA) and 4-mercaptohydrocinnamic acid (4-MHCA) are studied using cyclic voltammetry and ER spectroscopy. A monolayer of cyt c in a stable, electroactive state is formed on close-packed monolayers of both aromatic thiols. The redox potentials of cyt c are 35 mV for 4-MBA and 20 mV vs. Ag/AgCl for 4-MHCA. The electron transfer (ET) rate constants, ket, of cyt c at pH 7 are determined from the frequency dependence of the ER response to be 1800 s-1 for 4-MBA system and 3900 s-1 for 4-MHCA. The ket of adsorbed cyt c tends to be larger on the SAMs of both aromatic thiols than on the SAM of 6-mercaptohexanoic acid having a similar thickness, suggesting that the difference in the SAM structure is reflected to the ket of cyt c.

Original languageEnglish
Pages (from-to)467-470
Number of pages4
JournalDenki Kagaku
Volume65
Issue number6
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Redox reactions
Self assembled monolayers
Cytochromes c
Proteins
Electrodes
Acids
Sulfhydryl Compounds
Monolayers
Cyclic voltammetry
Rate constants
Spectroscopy
Electrons
4-mercaptobenzoate

Keywords

  • Cyt c
  • ER spectroscopy
  • Mercaptobenzoic acid
  • Self-assembled monolayer

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Redox reactions of cytochrome c on self-assembled monolayers of mercaptobenzoic acid and mercaptohydrocinnamic acid adsorbed on Au (111) electrodes. / Imabayashi, Shinichiro; Mita, Takahiro; Feng, Zhi Qiang; Iida, Minehiko; Niki, Katsumi; Kakiuchi, Takashi.

In: Denki Kagaku, Vol. 65, No. 6, 1997, p. 467-470.

Research output: Contribution to journalArticle

Imabayashi, Shinichiro ; Mita, Takahiro ; Feng, Zhi Qiang ; Iida, Minehiko ; Niki, Katsumi ; Kakiuchi, Takashi. / Redox reactions of cytochrome c on self-assembled monolayers of mercaptobenzoic acid and mercaptohydrocinnamic acid adsorbed on Au (111) electrodes. In: Denki Kagaku. 1997 ; Vol. 65, No. 6. pp. 467-470.
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AU - Mita, Takahiro

AU - Feng, Zhi Qiang

AU - Iida, Minehiko

AU - Niki, Katsumi

AU - Kakiuchi, Takashi

PY - 1997

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N2 - The redox reactions of cytochrome c (cyt c) adsorbed on self-assembled monolayers (SAMs) of 4-mercaptobenzoic acid (4-MBA) and 4-mercaptohydrocinnamic acid (4-MHCA) are studied using cyclic voltammetry and ER spectroscopy. A monolayer of cyt c in a stable, electroactive state is formed on close-packed monolayers of both aromatic thiols. The redox potentials of cyt c are 35 mV for 4-MBA and 20 mV vs. Ag/AgCl for 4-MHCA. The electron transfer (ET) rate constants, ket, of cyt c at pH 7 are determined from the frequency dependence of the ER response to be 1800 s-1 for 4-MBA system and 3900 s-1 for 4-MHCA. The ket of adsorbed cyt c tends to be larger on the SAMs of both aromatic thiols than on the SAM of 6-mercaptohexanoic acid having a similar thickness, suggesting that the difference in the SAM structure is reflected to the ket of cyt c.

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