Regulation of melanoblast and retinal pigment epithelium development by Xenopus laevis Mitf

Mayuko Kumasaka, Shigeru Sato, Ichiro Yajima, Colin R. Goding, Hiroaki Yamamoto

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Mitf is a central regulator of pigment cell development that is essential for the normal development of the melanocyte and retinal pigment epithelium (RPE) lineages. To understand better the role of Mitf, we have used the Xenopus laevis experimental system to allow a rapid examination of the role of Mitf in vivo. Here, we report the function of XlMitfα-M on melanophore development and melanization compared with that of Slug that is expressed in neural crest cells. Overexpression of XlMitfα-M led to an increase in melanophores that was partly contributed by an increase in Slug-positive cells, indicating that XlMitfα-M is a key regulator of melanocyte/melanophore development and melanization. Moreover, overexpression of a dominant-negative form of XlMitfα led to a decrease in the number of melanophores and induced abnormal melanoblast migration. We also observed an induction of ectopic RPE and extended RPE by overexpression of XlMitfα-M and possible interactions between XlMitfα and several eye-related genes essential for normal eye development.

Original languageEnglish
Pages (from-to)523-534
Number of pages12
JournalDevelopmental Dynamics
Volume234
Issue number3
DOIs
Publication statusPublished - 2005 Nov 1
Externally publishedYes

Keywords

  • Dct
  • Eye
  • Melanin
  • Melanocyte
  • Melanophore
  • Microphthalmia
  • Mitf
  • Pigment cell
  • Retinal pigment epithelium
  • Slug
  • Sox10
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Regulation of melanoblast and retinal pigment epithelium development by Xenopus laevis Mitf. / Kumasaka, Mayuko; Sato, Shigeru; Yajima, Ichiro; Goding, Colin R.; Yamamoto, Hiroaki.

In: Developmental Dynamics, Vol. 234, No. 3, 01.11.2005, p. 523-534.

Research output: Contribution to journalArticle

Kumasaka, Mayuko ; Sato, Shigeru ; Yajima, Ichiro ; Goding, Colin R. ; Yamamoto, Hiroaki. / Regulation of melanoblast and retinal pigment epithelium development by Xenopus laevis Mitf. In: Developmental Dynamics. 2005 ; Vol. 234, No. 3. pp. 523-534.
@article{c23a9e799dff49ed849b7968f4f4dad9,
title = "Regulation of melanoblast and retinal pigment epithelium development by Xenopus laevis Mitf",
abstract = "Mitf is a central regulator of pigment cell development that is essential for the normal development of the melanocyte and retinal pigment epithelium (RPE) lineages. To understand better the role of Mitf, we have used the Xenopus laevis experimental system to allow a rapid examination of the role of Mitf in vivo. Here, we report the function of XlMitfα-M on melanophore development and melanization compared with that of Slug that is expressed in neural crest cells. Overexpression of XlMitfα-M led to an increase in melanophores that was partly contributed by an increase in Slug-positive cells, indicating that XlMitfα-M is a key regulator of melanocyte/melanophore development and melanization. Moreover, overexpression of a dominant-negative form of XlMitfα led to a decrease in the number of melanophores and induced abnormal melanoblast migration. We also observed an induction of ectopic RPE and extended RPE by overexpression of XlMitfα-M and possible interactions between XlMitfα and several eye-related genes essential for normal eye development.",
keywords = "Dct, Eye, Melanin, Melanocyte, Melanophore, Microphthalmia, Mitf, Pigment cell, Retinal pigment epithelium, Slug, Sox10, Xenopus",
author = "Mayuko Kumasaka and Shigeru Sato and Ichiro Yajima and Goding, {Colin R.} and Hiroaki Yamamoto",
year = "2005",
month = "11",
day = "1",
doi = "10.1002/dvdy.20505",
language = "English",
volume = "234",
pages = "523--534",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Regulation of melanoblast and retinal pigment epithelium development by Xenopus laevis Mitf

AU - Kumasaka, Mayuko

AU - Sato, Shigeru

AU - Yajima, Ichiro

AU - Goding, Colin R.

AU - Yamamoto, Hiroaki

PY - 2005/11/1

Y1 - 2005/11/1

N2 - Mitf is a central regulator of pigment cell development that is essential for the normal development of the melanocyte and retinal pigment epithelium (RPE) lineages. To understand better the role of Mitf, we have used the Xenopus laevis experimental system to allow a rapid examination of the role of Mitf in vivo. Here, we report the function of XlMitfα-M on melanophore development and melanization compared with that of Slug that is expressed in neural crest cells. Overexpression of XlMitfα-M led to an increase in melanophores that was partly contributed by an increase in Slug-positive cells, indicating that XlMitfα-M is a key regulator of melanocyte/melanophore development and melanization. Moreover, overexpression of a dominant-negative form of XlMitfα led to a decrease in the number of melanophores and induced abnormal melanoblast migration. We also observed an induction of ectopic RPE and extended RPE by overexpression of XlMitfα-M and possible interactions between XlMitfα and several eye-related genes essential for normal eye development.

AB - Mitf is a central regulator of pigment cell development that is essential for the normal development of the melanocyte and retinal pigment epithelium (RPE) lineages. To understand better the role of Mitf, we have used the Xenopus laevis experimental system to allow a rapid examination of the role of Mitf in vivo. Here, we report the function of XlMitfα-M on melanophore development and melanization compared with that of Slug that is expressed in neural crest cells. Overexpression of XlMitfα-M led to an increase in melanophores that was partly contributed by an increase in Slug-positive cells, indicating that XlMitfα-M is a key regulator of melanocyte/melanophore development and melanization. Moreover, overexpression of a dominant-negative form of XlMitfα led to a decrease in the number of melanophores and induced abnormal melanoblast migration. We also observed an induction of ectopic RPE and extended RPE by overexpression of XlMitfα-M and possible interactions between XlMitfα and several eye-related genes essential for normal eye development.

KW - Dct

KW - Eye

KW - Melanin

KW - Melanocyte

KW - Melanophore

KW - Microphthalmia

KW - Mitf

KW - Pigment cell

KW - Retinal pigment epithelium

KW - Slug

KW - Sox10

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=27444440806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27444440806&partnerID=8YFLogxK

U2 - 10.1002/dvdy.20505

DO - 10.1002/dvdy.20505

M3 - Article

VL - 234

SP - 523

EP - 534

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 3

ER -