Abstract
The protective activity of rosmarinic acid from Perilla frutescens on liver injury induced by LPS in D-GalN-sensitized mice was examined. We also investigated the effects of antitumor necrosis factor-α antibody (anti-TNF), superoxide dismutase (SOD), and aminoguanidine (AG) on this model in order to elucidate the mechanism of rosmarinic acid protection. Perilla extract (PE) and rosmarinic acid (RA) treatments significantly reduced the elevation of plasma asparatate aminotransferase levels, as well as anti-TNF and SOD treatment, compared with controls, but this reduction was not seen in the AG group. These results were confirmed by histological examination using hematoxylin-eosin and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. Increases in tumor necrosis factor-α (TNF-α) mRNA expression in liver and in plasma TNF-α levels, which were observed in control mice, were not significantly reduced by PE or RA. PE and RA treatments also did not significantly diminish iNOS mRNA expression or plasma nitrate/nitrite levels. Nitrotyrosine and Nε-(hexanonyl)lysine (HEL) production, the residue of oxidative stress, was observed in livers from controls, but not in those mice pretreated with PE or RA. These results suggest that the liver protection of RA is due to the scavenging or reducing activities-superoxide or peroxynitirite rather than to inhibition of TNF-α production.
| Original language | English |
|---|---|
| Pages (from-to) | 798-806 |
| Number of pages | 9 |
| Journal | Free Radical Biology and Medicine |
| Volume | 33 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2002 Sep 15 |
| Externally published | Yes |
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Keywords
- Free radicals
- Liver injury
- N-(hexanonyl)lysine
- Nitrotyrosine
- Perilla
- Rosmarinic acid
ASJC Scopus subject areas
- Medicine(all)
- Toxicology
- Clinical Biochemistry
Cite this
Rosmarinic acid, a major polyphenolic component of Perilla frutescens, reduces lipopolysaccharide (LPS)-induced liver injury in D-galactosamine (D-GalN)-sensitized mice. / Osakabe, Naomi; Yasuda, Akiko; Natsume, Midori; Sanbongi, Chiaki; Kato, Yoji; Osawa, Toshihiko; Yoshikawa, Toshikazu.
In: Free Radical Biology and Medicine, Vol. 33, No. 6, 15.09.2002, p. 798-806.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rosmarinic acid, a major polyphenolic component of Perilla frutescens, reduces lipopolysaccharide (LPS)-induced liver injury in D-galactosamine (D-GalN)-sensitized mice
AU - Osakabe, Naomi
AU - Yasuda, Akiko
AU - Natsume, Midori
AU - Sanbongi, Chiaki
AU - Kato, Yoji
AU - Osawa, Toshihiko
AU - Yoshikawa, Toshikazu
PY - 2002/9/15
Y1 - 2002/9/15
N2 - The protective activity of rosmarinic acid from Perilla frutescens on liver injury induced by LPS in D-GalN-sensitized mice was examined. We also investigated the effects of antitumor necrosis factor-α antibody (anti-TNF), superoxide dismutase (SOD), and aminoguanidine (AG) on this model in order to elucidate the mechanism of rosmarinic acid protection. Perilla extract (PE) and rosmarinic acid (RA) treatments significantly reduced the elevation of plasma asparatate aminotransferase levels, as well as anti-TNF and SOD treatment, compared with controls, but this reduction was not seen in the AG group. These results were confirmed by histological examination using hematoxylin-eosin and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. Increases in tumor necrosis factor-α (TNF-α) mRNA expression in liver and in plasma TNF-α levels, which were observed in control mice, were not significantly reduced by PE or RA. PE and RA treatments also did not significantly diminish iNOS mRNA expression or plasma nitrate/nitrite levels. Nitrotyrosine and Nε-(hexanonyl)lysine (HEL) production, the residue of oxidative stress, was observed in livers from controls, but not in those mice pretreated with PE or RA. These results suggest that the liver protection of RA is due to the scavenging or reducing activities-superoxide or peroxynitirite rather than to inhibition of TNF-α production.
AB - The protective activity of rosmarinic acid from Perilla frutescens on liver injury induced by LPS in D-GalN-sensitized mice was examined. We also investigated the effects of antitumor necrosis factor-α antibody (anti-TNF), superoxide dismutase (SOD), and aminoguanidine (AG) on this model in order to elucidate the mechanism of rosmarinic acid protection. Perilla extract (PE) and rosmarinic acid (RA) treatments significantly reduced the elevation of plasma asparatate aminotransferase levels, as well as anti-TNF and SOD treatment, compared with controls, but this reduction was not seen in the AG group. These results were confirmed by histological examination using hematoxylin-eosin and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. Increases in tumor necrosis factor-α (TNF-α) mRNA expression in liver and in plasma TNF-α levels, which were observed in control mice, were not significantly reduced by PE or RA. PE and RA treatments also did not significantly diminish iNOS mRNA expression or plasma nitrate/nitrite levels. Nitrotyrosine and Nε-(hexanonyl)lysine (HEL) production, the residue of oxidative stress, was observed in livers from controls, but not in those mice pretreated with PE or RA. These results suggest that the liver protection of RA is due to the scavenging or reducing activities-superoxide or peroxynitirite rather than to inhibition of TNF-α production.
KW - Free radicals
KW - Liver injury
KW - N-(hexanonyl)lysine
KW - Nitrotyrosine
KW - Perilla
KW - Rosmarinic acid
UR - http://www.scopus.com/inward/record.url?scp=0037105253&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037105253&partnerID=8YFLogxK
U2 - 10.1016/S0891-5849(02)00970-X
DO - 10.1016/S0891-5849(02)00970-X
M3 - Article
C2 - 12208367
AN - SCOPUS:0037105253
VL - 33
SP - 798
EP - 806
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
IS - 6
ER -