Rosmarinic acid, a major polyphenolic component of Perilla frutescens, reduces lipopolysaccharide (LPS)-induced liver injury in D-galactosamine (D-GalN)-sensitized mice

Naomi Osakabe, Akiko Yasuda, Midori Natsume, Chiaki Sanbongi, Yoji Kato, Toshihiko Osawa, Toshikazu Yoshikawa

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

The protective activity of rosmarinic acid from Perilla frutescens on liver injury induced by LPS in D-GalN-sensitized mice was examined. We also investigated the effects of antitumor necrosis factor-α antibody (anti-TNF), superoxide dismutase (SOD), and aminoguanidine (AG) on this model in order to elucidate the mechanism of rosmarinic acid protection. Perilla extract (PE) and rosmarinic acid (RA) treatments significantly reduced the elevation of plasma asparatate aminotransferase levels, as well as anti-TNF and SOD treatment, compared with controls, but this reduction was not seen in the AG group. These results were confirmed by histological examination using hematoxylin-eosin and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. Increases in tumor necrosis factor-α (TNF-α) mRNA expression in liver and in plasma TNF-α levels, which were observed in control mice, were not significantly reduced by PE or RA. PE and RA treatments also did not significantly diminish iNOS mRNA expression or plasma nitrate/nitrite levels. Nitrotyrosine and Nε-(hexanonyl)lysine (HEL) production, the residue of oxidative stress, was observed in livers from controls, but not in those mice pretreated with PE or RA. These results suggest that the liver protection of RA is due to the scavenging or reducing activities-superoxide or peroxynitirite rather than to inhibition of TNF-α production.

Original languageEnglish
Pages (from-to)798-806
Number of pages9
JournalFree Radical Biology and Medicine
Volume33
Issue number6
DOIs
Publication statusPublished - 2002 Sep 15
Externally publishedYes

Fingerprint

Galactosamine
Liver
Lipopolysaccharides
Tumor Necrosis Factor-alpha
Plasmas
Superoxide Dismutase
Messenger RNA
Oxidative stress
DNA Nucleotidylexotransferase
Antibodies
Scavenging
Hematoxylin
Eosine Yellowish-(YS)
Nitrites
Transaminases
rosmarinic acid
Superoxides
Nitrates
Labeling
Lysine

Keywords

  • Free radicals
  • Liver injury
  • N-(hexanonyl)lysine
  • Nitrotyrosine
  • Perilla
  • Rosmarinic acid

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Rosmarinic acid, a major polyphenolic component of Perilla frutescens, reduces lipopolysaccharide (LPS)-induced liver injury in D-galactosamine (D-GalN)-sensitized mice. / Osakabe, Naomi; Yasuda, Akiko; Natsume, Midori; Sanbongi, Chiaki; Kato, Yoji; Osawa, Toshihiko; Yoshikawa, Toshikazu.

In: Free Radical Biology and Medicine, Vol. 33, No. 6, 15.09.2002, p. 798-806.

Research output: Contribution to journalArticle

Osakabe, Naomi ; Yasuda, Akiko ; Natsume, Midori ; Sanbongi, Chiaki ; Kato, Yoji ; Osawa, Toshihiko ; Yoshikawa, Toshikazu. / Rosmarinic acid, a major polyphenolic component of Perilla frutescens, reduces lipopolysaccharide (LPS)-induced liver injury in D-galactosamine (D-GalN)-sensitized mice. In: Free Radical Biology and Medicine. 2002 ; Vol. 33, No. 6. pp. 798-806.
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AU - Natsume, Midori

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