Selective adsorption of an antibody onto a plasma-polymerized film for protein patterning

Atsunori Hiratsuka, Naoya Murata, Hitoshi Muguruma, Kazunari Matsumura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Techniques for patterned modification of substrate sur faces are important for forming microarrays on protein chips. A hexam-ethyldisiloxane plasma-polymerized film (HMDS PPF) was deposited on a glass substrate and the resulting surface was partially modified by sub sequent nitrogen plasma treatment with a patterned shadow mask. When surface adsorption of an antibody protein (F(ab')2 fragment of anti-human immunoglobulin G) was visualized by fluorescence microscopy, distinct 80 x 80 μm2 square spots were observed, surrounded by a non-fluorescent 80 μmi-wide grid. This pattern could be attributed to proteins selectively ad sorbed onto the nitrogen plasma-treated regions but not onto the surface of pristine HMDS PPF. This provided a simple fabrication method of protein patterning.

Original languageEnglish
Pages (from-to)978-980
Number of pages3
JournalIEICE Transactions on Electronics
VolumeE91-C
Issue number6
DOIs
Publication statusPublished - 2008 Jun

Fingerprint

Antibodies
Proteins
Nitrogen plasma
Plasmas
Adsorption
Fluorescence microscopy
Substrates
Microarrays
Masks
Immunoglobulin G
Fabrication
Glass

Keywords

  • Adsorp tion
  • Antibody
  • F(ab') fragment
  • Patterning
  • Plasma-polymerized film

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials

Cite this

Selective adsorption of an antibody onto a plasma-polymerized film for protein patterning. / Hiratsuka, Atsunori; Murata, Naoya; Muguruma, Hitoshi; Matsumura, Kazunari.

In: IEICE Transactions on Electronics, Vol. E91-C, No. 6, 06.2008, p. 978-980.

Research output: Contribution to journalArticle

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