Sequential Injection Analysis of Butyrylcholinesterase Using Butyrylcholine Ion-Selective Electrode Detector

Narimasa Koizumi; Ryusei Nishiyama; Takashi Masadome

doi:10.5796/ELECTROCHEMISTRY.22-00095

Sequential Injection Analysis of Butyrylcholinesterase Using Butyrylcholine Ion-Selective Electrode Detector

Narimasa Koizumi, Ryusei Nishiyama, Takashi Masadome

Research output: Contribution to journal › Article › peer-review

Abstract

A new automated analytical method for the determination of Butyrylcholinesterase (BChE) based on sequential injection analysis (SIA) method using a butyrylcholine chloride (BuCh) ion-selective electrode (ISE) detector was developed. BuCh is degraded to choline and butyrate by the enzymatic activity of BChE, resulting in a decrease in BuCh concentration. By detecting this change in BuCh concentration with BuCh-ISE, BChE can be determined indirectly. In the present SIA method using the BuCh-ISE detector based on α-cyclodextrin membrane, BChE in the concentration range of 0.015–0.50 units/mL was determined at a BuCh concentration of 2.0 mmol dm⁻³ and a reaction time of 5.0 min. The detection limit for BChE is 4.8 × 10⁻³ units/mL.

Original language	English
Article number	097003
Journal	Electrochemistry
Volume	90
Issue number	9
DOIs	https://doi.org/10.5796/ELECTROCHEMISTRY.22-00095
Publication status	Published - 2022

Keywords

Butyrylcholinesterase
Enzyme
Ion-Selective Electrode
Sequential Injection Analysis

ASJC Scopus subject areas

Electrochemistry

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10.5796/ELECTROCHEMISTRY.22-00095

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@article{9aa11e904ae24cd493b4f537f9676665,

title = "Sequential Injection Analysis of Butyrylcholinesterase Using Butyrylcholine Ion-Selective Electrode Detector",

abstract = "A new automated analytical method for the determination of Butyrylcholinesterase (BChE) based on sequential injection analysis (SIA) method using a butyrylcholine chloride (BuCh) ion-selective electrode (ISE) detector was developed. BuCh is degraded to choline and butyrate by the enzymatic activity of BChE, resulting in a decrease in BuCh concentration. By detecting this change in BuCh concentration with BuCh-ISE, BChE can be determined indirectly. In the present SIA method using the BuCh-ISE detector based on α-cyclodextrin membrane, BChE in the concentration range of 0.015–0.50 units/mL was determined at a BuCh concentration of 2.0 mmol dm−3 and a reaction time of 5.0 min. The detection limit for BChE is 4.8 × 10−3 units/mL.",

keywords = "Butyrylcholinesterase, Enzyme, Ion-Selective Electrode, Sequential Injection Analysis",

author = "Narimasa Koizumi and Ryusei Nishiyama and Takashi Masadome",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2022. Published by ECSJ.",

year = "2022",

doi = "10.5796/ELECTROCHEMISTRY.22-00095",

language = "English",

volume = "90",

journal = "Electrochemistry",

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T1 - Sequential Injection Analysis of Butyrylcholinesterase Using Butyrylcholine Ion-Selective Electrode Detector

AU - Koizumi, Narimasa

AU - Nishiyama, Ryusei

AU - Masadome, Takashi

N1 - Publisher Copyright: © The Author(s) 2022. Published by ECSJ.

PY - 2022

Y1 - 2022

N2 - A new automated analytical method for the determination of Butyrylcholinesterase (BChE) based on sequential injection analysis (SIA) method using a butyrylcholine chloride (BuCh) ion-selective electrode (ISE) detector was developed. BuCh is degraded to choline and butyrate by the enzymatic activity of BChE, resulting in a decrease in BuCh concentration. By detecting this change in BuCh concentration with BuCh-ISE, BChE can be determined indirectly. In the present SIA method using the BuCh-ISE detector based on α-cyclodextrin membrane, BChE in the concentration range of 0.015–0.50 units/mL was determined at a BuCh concentration of 2.0 mmol dm−3 and a reaction time of 5.0 min. The detection limit for BChE is 4.8 × 10−3 units/mL.

AB - A new automated analytical method for the determination of Butyrylcholinesterase (BChE) based on sequential injection analysis (SIA) method using a butyrylcholine chloride (BuCh) ion-selective electrode (ISE) detector was developed. BuCh is degraded to choline and butyrate by the enzymatic activity of BChE, resulting in a decrease in BuCh concentration. By detecting this change in BuCh concentration with BuCh-ISE, BChE can be determined indirectly. In the present SIA method using the BuCh-ISE detector based on α-cyclodextrin membrane, BChE in the concentration range of 0.015–0.50 units/mL was determined at a BuCh concentration of 2.0 mmol dm−3 and a reaction time of 5.0 min. The detection limit for BChE is 4.8 × 10−3 units/mL.

KW - Butyrylcholinesterase

KW - Enzyme

KW - Ion-Selective Electrode

KW - Sequential Injection Analysis

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U2 - 10.5796/ELECTROCHEMISTRY.22-00095

DO - 10.5796/ELECTROCHEMISTRY.22-00095

M3 - Article

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SN - 1344-3542

VL - 90

JO - Electrochemistry

JF - Electrochemistry

IS - 9

M1 - 097003

ER -

Sequential Injection Analysis of Butyrylcholinesterase Using Butyrylcholine Ion-Selective Electrode Detector

Abstract

Keywords

ASJC Scopus subject areas

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