Shear-stress mediated nitric oxide production within red blood cells: A dose-response

Jarod T. Horobin, Nobuo Watanabe, Masaya Hakozaki, Surendran Sabapathy, Michael J. Simmonds

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Red blood cells (RBC) are exposed to varying shear stress while traversing the circulatory system; this shear initiates RBC-derived nitric oxide (NO) production. OBJECTIVE: The current study investigated the effect of varying shear stress dose on RBC-derived NO production. METHODS: Separated RBC were prepared with the molecular probe, diamino-fluoreoscein diacetate, for fluorometric detection of NO. Prepared RBC were exposed to discrete magnitudes of shear stress (1-100Pa), and intracellular and extracellular fluorescence was quantified via fluorescence microscopy at baseline (0min) and discrete time-points (1-30min). RESULTS: Intracellular RBC-derived NO fluorescence was significantly increased (p<0.05) following shear stress exposure when compared to baseline at: i) 1min-100Pa; ii) 5min-1, 5Pa; iii) 15min-1, 5, 35Pa; iv) 30min-35Pa. Extracellular RBC-derived NO fluorescence was significantly increased (p<0.05) following shear stress exposure when compared to baseline at: i) 5min - 100Pa; ii) 15min-100Pa; iii) 30min-40, 100Pa. CONCLUSIONS: These data indicate that: i) a dose-response exists for the RBC-derived production of NO via shear stress; and ii) exposure to supra-physiological shear stress allows for the leakage of RBC intracellular contents (e.g., RBC-derived NO).

Original languageEnglish
Pages (from-to)203-214
Number of pages12
JournalClinical Hemorheology and Microcirculation
Volume71
Issue number2
DOIs
Publication statusPublished - 2019 Jan 1

Keywords

  • biocompatibility
  • erythrocyte
  • Haemorheology
  • mechanotransduction
  • vasodilation

ASJC Scopus subject areas

  • Physiology
  • Hematology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Shear-stress mediated nitric oxide production within red blood cells : A dose-response. / Horobin, Jarod T.; Watanabe, Nobuo; Hakozaki, Masaya; Sabapathy, Surendran; Simmonds, Michael J.

In: Clinical Hemorheology and Microcirculation, Vol. 71, No. 2, 01.01.2019, p. 203-214.

Research output: Contribution to journalArticle

Horobin, Jarod T. ; Watanabe, Nobuo ; Hakozaki, Masaya ; Sabapathy, Surendran ; Simmonds, Michael J. / Shear-stress mediated nitric oxide production within red blood cells : A dose-response. In: Clinical Hemorheology and Microcirculation. 2019 ; Vol. 71, No. 2. pp. 203-214.
@article{38e826f1f7b04c99be2cfb39ef08cb00,
title = "Shear-stress mediated nitric oxide production within red blood cells: A dose-response",
abstract = "BACKGROUND: Red blood cells (RBC) are exposed to varying shear stress while traversing the circulatory system; this shear initiates RBC-derived nitric oxide (NO) production. OBJECTIVE: The current study investigated the effect of varying shear stress dose on RBC-derived NO production. METHODS: Separated RBC were prepared with the molecular probe, diamino-fluoreoscein diacetate, for fluorometric detection of NO. Prepared RBC were exposed to discrete magnitudes of shear stress (1-100Pa), and intracellular and extracellular fluorescence was quantified via fluorescence microscopy at baseline (0min) and discrete time-points (1-30min). RESULTS: Intracellular RBC-derived NO fluorescence was significantly increased (p<0.05) following shear stress exposure when compared to baseline at: i) 1min-100Pa; ii) 5min-1, 5Pa; iii) 15min-1, 5, 35Pa; iv) 30min-35Pa. Extracellular RBC-derived NO fluorescence was significantly increased (p<0.05) following shear stress exposure when compared to baseline at: i) 5min - 100Pa; ii) 15min-100Pa; iii) 30min-40, 100Pa. CONCLUSIONS: These data indicate that: i) a dose-response exists for the RBC-derived production of NO via shear stress; and ii) exposure to supra-physiological shear stress allows for the leakage of RBC intracellular contents (e.g., RBC-derived NO).",
keywords = "biocompatibility, erythrocyte, Haemorheology, mechanotransduction, vasodilation",
author = "Horobin, {Jarod T.} and Nobuo Watanabe and Masaya Hakozaki and Surendran Sabapathy and Simmonds, {Michael J.}",
year = "2019",
month = "1",
day = "1",
doi = "10.3233/CH-189412",
language = "English",
volume = "71",
pages = "203--214",
journal = "Clinical Hemorheology and Microcirculation",
issn = "1386-0291",
publisher = "IOS Press",
number = "2",

}

TY - JOUR

T1 - Shear-stress mediated nitric oxide production within red blood cells

T2 - A dose-response

AU - Horobin, Jarod T.

AU - Watanabe, Nobuo

AU - Hakozaki, Masaya

AU - Sabapathy, Surendran

AU - Simmonds, Michael J.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - BACKGROUND: Red blood cells (RBC) are exposed to varying shear stress while traversing the circulatory system; this shear initiates RBC-derived nitric oxide (NO) production. OBJECTIVE: The current study investigated the effect of varying shear stress dose on RBC-derived NO production. METHODS: Separated RBC were prepared with the molecular probe, diamino-fluoreoscein diacetate, for fluorometric detection of NO. Prepared RBC were exposed to discrete magnitudes of shear stress (1-100Pa), and intracellular and extracellular fluorescence was quantified via fluorescence microscopy at baseline (0min) and discrete time-points (1-30min). RESULTS: Intracellular RBC-derived NO fluorescence was significantly increased (p<0.05) following shear stress exposure when compared to baseline at: i) 1min-100Pa; ii) 5min-1, 5Pa; iii) 15min-1, 5, 35Pa; iv) 30min-35Pa. Extracellular RBC-derived NO fluorescence was significantly increased (p<0.05) following shear stress exposure when compared to baseline at: i) 5min - 100Pa; ii) 15min-100Pa; iii) 30min-40, 100Pa. CONCLUSIONS: These data indicate that: i) a dose-response exists for the RBC-derived production of NO via shear stress; and ii) exposure to supra-physiological shear stress allows for the leakage of RBC intracellular contents (e.g., RBC-derived NO).

AB - BACKGROUND: Red blood cells (RBC) are exposed to varying shear stress while traversing the circulatory system; this shear initiates RBC-derived nitric oxide (NO) production. OBJECTIVE: The current study investigated the effect of varying shear stress dose on RBC-derived NO production. METHODS: Separated RBC were prepared with the molecular probe, diamino-fluoreoscein diacetate, for fluorometric detection of NO. Prepared RBC were exposed to discrete magnitudes of shear stress (1-100Pa), and intracellular and extracellular fluorescence was quantified via fluorescence microscopy at baseline (0min) and discrete time-points (1-30min). RESULTS: Intracellular RBC-derived NO fluorescence was significantly increased (p<0.05) following shear stress exposure when compared to baseline at: i) 1min-100Pa; ii) 5min-1, 5Pa; iii) 15min-1, 5, 35Pa; iv) 30min-35Pa. Extracellular RBC-derived NO fluorescence was significantly increased (p<0.05) following shear stress exposure when compared to baseline at: i) 5min - 100Pa; ii) 15min-100Pa; iii) 30min-40, 100Pa. CONCLUSIONS: These data indicate that: i) a dose-response exists for the RBC-derived production of NO via shear stress; and ii) exposure to supra-physiological shear stress allows for the leakage of RBC intracellular contents (e.g., RBC-derived NO).

KW - biocompatibility

KW - erythrocyte

KW - Haemorheology

KW - mechanotransduction

KW - vasodilation

UR - http://www.scopus.com/inward/record.url?scp=85064138974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064138974&partnerID=8YFLogxK

U2 - 10.3233/CH-189412

DO - 10.3233/CH-189412

M3 - Article

C2 - 30584130

AN - SCOPUS:85064138974

VL - 71

SP - 203

EP - 214

JO - Clinical Hemorheology and Microcirculation

JF - Clinical Hemorheology and Microcirculation

SN - 1386-0291

IS - 2

ER -