Significance of alcohol dehydrogenase (ADH) as a marker of hepatic centrilobular injury: a biochemical and immunohistochemical study.

D. Ito, H. Ishii, S. Kato, T. Takagi, S. Takekawa, Sadakazu Aiso, M. Tsuchiya, R. Buehler, J. P. von Wartburg

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Since we demonstrated previously that alcohol dehydrogenase (ADH) is distributed predominantly in zone 3 of the hepatic acinus, we have investigated the usefulness of determinations of serum ADH activity in the assessment of hepatic injuries. The measurement of serum ADH activity appears to be useful for the detection of acute and early centrilobular hepatic damage, as deduced from the results obtained from experimental hepatic injuries produced by bromobenzene, hypoxia and acute administration of ethanol. Whereas serum ADH activity increased slightly after the generation of an acute ethanol load in rats which were given ethanol chronically, the hepatic content of ADH tended to decrease. Moreover, a rather selective reduction of hepatic ADH activity was found in zone 3 of the hepatic acinus. Thus, it is conceivable that chronic alcoholic injury to the liver may result in persistent release of ADH from hepatocytes resulting in a relatively low elevation of the level of the enzyme in the blood, rather than a sudden release of the enzyme leading to a sharp increase in the serum enzyme activity.

Original languageEnglish
Pages (from-to)523-527
Number of pages5
JournalAlcohol and alcoholism (Oxford, Oxfordshire)
VolumeSuppl 1
Publication statusPublished - 1987 Dec 1
Externally publishedYes

Fingerprint

Alcohol Dehydrogenase
Ethanol
Enzyme activity
Enzymes
Liver
Rats
Blood

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

Cite this

Significance of alcohol dehydrogenase (ADH) as a marker of hepatic centrilobular injury : a biochemical and immunohistochemical study. / Ito, D.; Ishii, H.; Kato, S.; Takagi, T.; Takekawa, S.; Aiso, Sadakazu; Tsuchiya, M.; Buehler, R.; von Wartburg, J. P.

In: Alcohol and alcoholism (Oxford, Oxfordshire), Vol. Suppl 1, 01.12.1987, p. 523-527.

Research output: Contribution to journalArticle

Ito, D, Ishii, H, Kato, S, Takagi, T, Takekawa, S, Aiso, S, Tsuchiya, M, Buehler, R & von Wartburg, JP 1987, 'Significance of alcohol dehydrogenase (ADH) as a marker of hepatic centrilobular injury: a biochemical and immunohistochemical study.', Alcohol and alcoholism (Oxford, Oxfordshire), vol. Suppl 1, pp. 523-527.
Ito, D. ; Ishii, H. ; Kato, S. ; Takagi, T. ; Takekawa, S. ; Aiso, Sadakazu ; Tsuchiya, M. ; Buehler, R. ; von Wartburg, J. P. / Significance of alcohol dehydrogenase (ADH) as a marker of hepatic centrilobular injury : a biochemical and immunohistochemical study. In: Alcohol and alcoholism (Oxford, Oxfordshire). 1987 ; Vol. Suppl 1. pp. 523-527.
@article{2c6ff993c68545bf911a249420a3c9e1,
title = "Significance of alcohol dehydrogenase (ADH) as a marker of hepatic centrilobular injury: a biochemical and immunohistochemical study.",
abstract = "Since we demonstrated previously that alcohol dehydrogenase (ADH) is distributed predominantly in zone 3 of the hepatic acinus, we have investigated the usefulness of determinations of serum ADH activity in the assessment of hepatic injuries. The measurement of serum ADH activity appears to be useful for the detection of acute and early centrilobular hepatic damage, as deduced from the results obtained from experimental hepatic injuries produced by bromobenzene, hypoxia and acute administration of ethanol. Whereas serum ADH activity increased slightly after the generation of an acute ethanol load in rats which were given ethanol chronically, the hepatic content of ADH tended to decrease. Moreover, a rather selective reduction of hepatic ADH activity was found in zone 3 of the hepatic acinus. Thus, it is conceivable that chronic alcoholic injury to the liver may result in persistent release of ADH from hepatocytes resulting in a relatively low elevation of the level of the enzyme in the blood, rather than a sudden release of the enzyme leading to a sharp increase in the serum enzyme activity.",
author = "D. Ito and H. Ishii and S. Kato and T. Takagi and S. Takekawa and Sadakazu Aiso and M. Tsuchiya and R. Buehler and {von Wartburg}, {J. P.}",
year = "1987",
month = "12",
day = "1",
language = "English",
volume = "Suppl 1",
pages = "523--527",
journal = "Alcohol and Alcoholism",
issn = "0735-0414",
publisher = "Oxford University Press",

}

TY - JOUR

T1 - Significance of alcohol dehydrogenase (ADH) as a marker of hepatic centrilobular injury

T2 - a biochemical and immunohistochemical study.

AU - Ito, D.

AU - Ishii, H.

AU - Kato, S.

AU - Takagi, T.

AU - Takekawa, S.

AU - Aiso, Sadakazu

AU - Tsuchiya, M.

AU - Buehler, R.

AU - von Wartburg, J. P.

PY - 1987/12/1

Y1 - 1987/12/1

N2 - Since we demonstrated previously that alcohol dehydrogenase (ADH) is distributed predominantly in zone 3 of the hepatic acinus, we have investigated the usefulness of determinations of serum ADH activity in the assessment of hepatic injuries. The measurement of serum ADH activity appears to be useful for the detection of acute and early centrilobular hepatic damage, as deduced from the results obtained from experimental hepatic injuries produced by bromobenzene, hypoxia and acute administration of ethanol. Whereas serum ADH activity increased slightly after the generation of an acute ethanol load in rats which were given ethanol chronically, the hepatic content of ADH tended to decrease. Moreover, a rather selective reduction of hepatic ADH activity was found in zone 3 of the hepatic acinus. Thus, it is conceivable that chronic alcoholic injury to the liver may result in persistent release of ADH from hepatocytes resulting in a relatively low elevation of the level of the enzyme in the blood, rather than a sudden release of the enzyme leading to a sharp increase in the serum enzyme activity.

AB - Since we demonstrated previously that alcohol dehydrogenase (ADH) is distributed predominantly in zone 3 of the hepatic acinus, we have investigated the usefulness of determinations of serum ADH activity in the assessment of hepatic injuries. The measurement of serum ADH activity appears to be useful for the detection of acute and early centrilobular hepatic damage, as deduced from the results obtained from experimental hepatic injuries produced by bromobenzene, hypoxia and acute administration of ethanol. Whereas serum ADH activity increased slightly after the generation of an acute ethanol load in rats which were given ethanol chronically, the hepatic content of ADH tended to decrease. Moreover, a rather selective reduction of hepatic ADH activity was found in zone 3 of the hepatic acinus. Thus, it is conceivable that chronic alcoholic injury to the liver may result in persistent release of ADH from hepatocytes resulting in a relatively low elevation of the level of the enzyme in the blood, rather than a sudden release of the enzyme leading to a sharp increase in the serum enzyme activity.

UR - http://www.scopus.com/inward/record.url?scp=0023494378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023494378&partnerID=8YFLogxK

M3 - Article

C2 - 3426727

AN - SCOPUS:0023494378

VL - Suppl 1

SP - 523

EP - 527

JO - Alcohol and Alcoholism

JF - Alcohol and Alcoholism

SN - 0735-0414

ER -