Single live-bacterial cell assay of promoter activity and regulation: Escherichia coli gcl promoter

Jun Teramoto, Yoko Yamanishi, El Shimy H Magdy, Akiko Hasegawa, Masahiro Nakajima, Tomohiro Shimada, Fumihito Arai, Toshio Fukuda, Akira Ishihama

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

For measurement of the promoter activity and regulation within single bacterial cells, we constructed various types of the cell chip. After gelation of portions of E. coli culture wihin the cell chip, we have succeeded, for the first time, the real-time single-bacterial cell assay of promoter activity. Here we applied this newly developed system for measurement of the activity and regulation of E. coli gcl promoter.

Original languageEnglish
Title of host publication20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science
Pages255-258
Number of pages4
DOIs
Publication statusPublished - 2009
Externally publishedYes
Event20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science - Nagoya
Duration: 2009 Nov 82009 Nov 11

Other

Other20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science
CityNagoya
Period09/11/809/11/11

Fingerprint

Escherichia coli
Assays
Gelation

ASJC Scopus subject areas

  • Computer Science(all)
  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Teramoto, J., Yamanishi, Y., Magdy, E. S. H., Hasegawa, A., Nakajima, M., Shimada, T., ... Ishihama, A. (2009). Single live-bacterial cell assay of promoter activity and regulation: Escherichia coli gcl promoter. In 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science (pp. 255-258). [5351949] https://doi.org/10.1109/MHS.2009.5351949

Single live-bacterial cell assay of promoter activity and regulation : Escherichia coli gcl promoter. / Teramoto, Jun; Yamanishi, Yoko; Magdy, El Shimy H; Hasegawa, Akiko; Nakajima, Masahiro; Shimada, Tomohiro; Arai, Fumihito; Fukuda, Toshio; Ishihama, Akira.

20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science. 2009. p. 255-258 5351949.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Teramoto, J, Yamanishi, Y, Magdy, ESH, Hasegawa, A, Nakajima, M, Shimada, T, Arai, F, Fukuda, T & Ishihama, A 2009, Single live-bacterial cell assay of promoter activity and regulation: Escherichia coli gcl promoter. in 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science., 5351949, pp. 255-258, 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science, Nagoya, 09/11/8. https://doi.org/10.1109/MHS.2009.5351949
Teramoto J, Yamanishi Y, Magdy ESH, Hasegawa A, Nakajima M, Shimada T et al. Single live-bacterial cell assay of promoter activity and regulation: Escherichia coli gcl promoter. In 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science. 2009. p. 255-258. 5351949 https://doi.org/10.1109/MHS.2009.5351949
Teramoto, Jun ; Yamanishi, Yoko ; Magdy, El Shimy H ; Hasegawa, Akiko ; Nakajima, Masahiro ; Shimada, Tomohiro ; Arai, Fumihito ; Fukuda, Toshio ; Ishihama, Akira. / Single live-bacterial cell assay of promoter activity and regulation : Escherichia coli gcl promoter. 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science. 2009. pp. 255-258
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