Soluble and particulate methane monooxygenase gene clusters in the marine methanotroph Methylomicrobium sp. strain NI

Takamichi Nakamura, Toshihiro Hoaki, Satoshi Hanada, Akihiko Maruyama, Yoichi Kamagata, Hiroyuki Fuse

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Soluble methane monooxygenase (sMMO) and particulate methane monooxygenase (pMMO) gene clusters in the marine methanotroph Methylomicrobium sp. strain NI were completely sequenced and analysed. Degenerated primers were newly designed and used to amplify the gene fragments containing intergenic mmoX-Y and mmoD-C regions and a partial pmoC region. Phylogenetic analysis of amino acid sequences deduced from mmoX and pmoA, as well as of 16S rRNA gene sequences, indicated that this strain was most closely related to the halotolerant methanotroph Methylomicrobium buryatense. There were putative σ54- and σ70-dependent promoter sequences upstream of the sMMO and pMMO genes, respectively, and mmoG, which is known to be related to the expression and assembly of sMMO, existed downstream of the sMMO genes. These findings suggest that the major components and regulation of MMOs in this marine methanotroph are quite similar to those in freshwater methane oxidizers, despite the difference in their habitats.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalFEMS Microbiology Letters
Volume277
Issue number2
DOIs
Publication statusPublished - 2007 Dec 1

Keywords

  • Marine methanotroph
  • Methane monooxygenase
  • Methylomicrobium
  • pMMO
  • sMMO

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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