Spatiotemporal gene control by the Cre-ERT2 system in melanocytes

Ichiro Yajima, Elodie Belloir, Yveline Bourgeois, Mayuko Kumasaka, Véronique Delmas, Lionel Larue

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The organ-specific and temporal control of gene activation/inactivation is a key issue in the understanding of protein function during normal and pathological development and during oncogenesis. We generated transgenic mice bearing a tamoxifen-dependent Cre recombinase (Tyr::Cre-ERT2) gene expressed under the control of a 6.1 kb murine tyrosinase promoter in order to facilitate targeted spatiotemporally controlled somatic recombination in melanoblasts/melanocytes. Cre-ERT2 production was detected in tissues containing melanocytes. After tamoxifen induction at various times during embryogenesis and adulthood in a Cre-responsive reporter mouse strain, genetic recombination was detected in the melanoblasts and melanocytes of the skin. Thus, the Tyr::Cre-ERT2 transgenic mice provides a valuable tool for following this cell lineage and for investigating gene function in melanocyte development and transformation.

Original languageEnglish
Pages (from-to)34-43
Number of pages10
JournalGenesis
Volume44
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1
Externally publishedYes

Keywords

  • Conditional mutagenesis
  • Development
  • Melanoblast
  • Mouse
  • Skin
  • Transformation
  • Tyrosinase
  • Vitiligo

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Yajima, I., Belloir, E., Bourgeois, Y., Kumasaka, M., Delmas, V., & Larue, L. (2006). Spatiotemporal gene control by the Cre-ERT2 system in melanocytes. Genesis, 44(1), 34-43. https://doi.org/10.1002/gene.20182

Spatiotemporal gene control by the Cre-ERT2 system in melanocytes. / Yajima, Ichiro; Belloir, Elodie; Bourgeois, Yveline; Kumasaka, Mayuko; Delmas, Véronique; Larue, Lionel.

In: Genesis, Vol. 44, No. 1, 01.01.2006, p. 34-43.

Research output: Contribution to journalArticle

Yajima, I, Belloir, E, Bourgeois, Y, Kumasaka, M, Delmas, V & Larue, L 2006, 'Spatiotemporal gene control by the Cre-ERT2 system in melanocytes', Genesis, vol. 44, no. 1, pp. 34-43. https://doi.org/10.1002/gene.20182
Yajima I, Belloir E, Bourgeois Y, Kumasaka M, Delmas V, Larue L. Spatiotemporal gene control by the Cre-ERT2 system in melanocytes. Genesis. 2006 Jan 1;44(1):34-43. https://doi.org/10.1002/gene.20182
Yajima, Ichiro ; Belloir, Elodie ; Bourgeois, Yveline ; Kumasaka, Mayuko ; Delmas, Véronique ; Larue, Lionel. / Spatiotemporal gene control by the Cre-ERT2 system in melanocytes. In: Genesis. 2006 ; Vol. 44, No. 1. pp. 34-43.
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