Sprouting from arteriovenous shunt vessels with increased blood flow

Y. Asano, Shigeru Ichioka, Masahiro Shibata, J. Ando, T. Nakatsuka

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the effect of increased blood flow on angiogenesis at the large vessels. The arteriovenous (AV) shunt was made on the thigh of male Wistar rats (n = 27) to increase blood flow, wrapped with artificial skin dermis, which consisted of a silicon outer layer, and isolated from surrounding tissues. Blood flow increased from 2.40 ± 0.77 to 35.8 ± 8.7 ml min-1 (14.9 times), and the shear stress index (relative value of shear stress) increased from 10.7 ± 3.6 to 73.4 ± 18.1 (6.85 times) 60 min after the shunt formation. Newly formed vessels were observed around the AV shunt loop. Scanning electron micrographs at the AV shunt vessel lumen showed modified endothelial cells at day 7 and a remarkable number of pores at day 14. The volume of newly formed vessels was increased 12 times from day 5 to day 14. The mechanical factor of shear stress was considered the major stimulator of angiogenesis. This is the first report of electron-microscopic observation of sprouts from a large vessel lumen. The new AV shunt model is useful for basic research on angiogenesis at the large vessels in vivo and, furthermore, could generate vascularised tissues with various cultured cells.

Original languageEnglish
Pages (from-to)126-130
Number of pages5
JournalMedical and Biological Engineering and Computing
Volume43
Issue number1
DOIs
Publication statusPublished - 2005 Jan
Externally publishedYes

Fingerprint

Shear stress
Blood
Tissue
Electrons
Endothelial cells
Rats
Skin
Cells
Scanning
Silicon

Keywords

  • Angiogenesis
  • Arteriovenous shunt
  • Increased blood flow
  • Shear stress
  • Sprouting

ASJC Scopus subject areas

  • Biomedical Engineering
  • Health Informatics
  • Health Information Management
  • Computer Science Applications
  • Computational Theory and Mathematics

Cite this

Sprouting from arteriovenous shunt vessels with increased blood flow. / Asano, Y.; Ichioka, Shigeru; Shibata, Masahiro; Ando, J.; Nakatsuka, T.

In: Medical and Biological Engineering and Computing, Vol. 43, No. 1, 01.2005, p. 126-130.

Research output: Contribution to journalArticle

Asano, Y. ; Ichioka, Shigeru ; Shibata, Masahiro ; Ando, J. ; Nakatsuka, T. / Sprouting from arteriovenous shunt vessels with increased blood flow. In: Medical and Biological Engineering and Computing. 2005 ; Vol. 43, No. 1. pp. 126-130.
@article{c4e4496460994f329990427c2bc4518a,
title = "Sprouting from arteriovenous shunt vessels with increased blood flow",
abstract = "The purpose of this study was to evaluate the effect of increased blood flow on angiogenesis at the large vessels. The arteriovenous (AV) shunt was made on the thigh of male Wistar rats (n = 27) to increase blood flow, wrapped with artificial skin dermis, which consisted of a silicon outer layer, and isolated from surrounding tissues. Blood flow increased from 2.40 ± 0.77 to 35.8 ± 8.7 ml min-1 (14.9 times), and the shear stress index (relative value of shear stress) increased from 10.7 ± 3.6 to 73.4 ± 18.1 (6.85 times) 60 min after the shunt formation. Newly formed vessels were observed around the AV shunt loop. Scanning electron micrographs at the AV shunt vessel lumen showed modified endothelial cells at day 7 and a remarkable number of pores at day 14. The volume of newly formed vessels was increased 12 times from day 5 to day 14. The mechanical factor of shear stress was considered the major stimulator of angiogenesis. This is the first report of electron-microscopic observation of sprouts from a large vessel lumen. The new AV shunt model is useful for basic research on angiogenesis at the large vessels in vivo and, furthermore, could generate vascularised tissues with various cultured cells.",
keywords = "Angiogenesis, Arteriovenous shunt, Increased blood flow, Shear stress, Sprouting",
author = "Y. Asano and Shigeru Ichioka and Masahiro Shibata and J. Ando and T. Nakatsuka",
year = "2005",
month = "1",
doi = "10.1007/BF02345133",
language = "English",
volume = "43",
pages = "126--130",
journal = "Medical and Biological Engineering and Computing",
issn = "0140-0118",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Sprouting from arteriovenous shunt vessels with increased blood flow

AU - Asano, Y.

AU - Ichioka, Shigeru

AU - Shibata, Masahiro

AU - Ando, J.

AU - Nakatsuka, T.

PY - 2005/1

Y1 - 2005/1

N2 - The purpose of this study was to evaluate the effect of increased blood flow on angiogenesis at the large vessels. The arteriovenous (AV) shunt was made on the thigh of male Wistar rats (n = 27) to increase blood flow, wrapped with artificial skin dermis, which consisted of a silicon outer layer, and isolated from surrounding tissues. Blood flow increased from 2.40 ± 0.77 to 35.8 ± 8.7 ml min-1 (14.9 times), and the shear stress index (relative value of shear stress) increased from 10.7 ± 3.6 to 73.4 ± 18.1 (6.85 times) 60 min after the shunt formation. Newly formed vessels were observed around the AV shunt loop. Scanning electron micrographs at the AV shunt vessel lumen showed modified endothelial cells at day 7 and a remarkable number of pores at day 14. The volume of newly formed vessels was increased 12 times from day 5 to day 14. The mechanical factor of shear stress was considered the major stimulator of angiogenesis. This is the first report of electron-microscopic observation of sprouts from a large vessel lumen. The new AV shunt model is useful for basic research on angiogenesis at the large vessels in vivo and, furthermore, could generate vascularised tissues with various cultured cells.

AB - The purpose of this study was to evaluate the effect of increased blood flow on angiogenesis at the large vessels. The arteriovenous (AV) shunt was made on the thigh of male Wistar rats (n = 27) to increase blood flow, wrapped with artificial skin dermis, which consisted of a silicon outer layer, and isolated from surrounding tissues. Blood flow increased from 2.40 ± 0.77 to 35.8 ± 8.7 ml min-1 (14.9 times), and the shear stress index (relative value of shear stress) increased from 10.7 ± 3.6 to 73.4 ± 18.1 (6.85 times) 60 min after the shunt formation. Newly formed vessels were observed around the AV shunt loop. Scanning electron micrographs at the AV shunt vessel lumen showed modified endothelial cells at day 7 and a remarkable number of pores at day 14. The volume of newly formed vessels was increased 12 times from day 5 to day 14. The mechanical factor of shear stress was considered the major stimulator of angiogenesis. This is the first report of electron-microscopic observation of sprouts from a large vessel lumen. The new AV shunt model is useful for basic research on angiogenesis at the large vessels in vivo and, furthermore, could generate vascularised tissues with various cultured cells.

KW - Angiogenesis

KW - Arteriovenous shunt

KW - Increased blood flow

KW - Shear stress

KW - Sprouting

UR - http://www.scopus.com/inward/record.url?scp=14044258536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14044258536&partnerID=8YFLogxK

U2 - 10.1007/BF02345133

DO - 10.1007/BF02345133

M3 - Article

VL - 43

SP - 126

EP - 130

JO - Medical and Biological Engineering and Computing

JF - Medical and Biological Engineering and Computing

SN - 0140-0118

IS - 1

ER -