Statistical optimisation of diesel biodegradation at low temperatures by an antarctic marine bacterial consortium isolated from non‐contaminated seawater

Nur Nadhirah Zakaria, Claudio Gomez‐fuentes, Khalilah Abdul Khalil, Peter Convey, Ahmad Fareez Ahmad Roslee, Azham Zulkharnain, Suriana Sabri, Noor Azmi Shaharuddin, Leyla Cárdenas, Siti Aqlima Ahmad

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Hydrocarbon pollution is widespread around the globe and, even in the remoteness of Antarctica, the impacts of hydrocarbons from anthropogenic sources are still apparent. Antarctica’s chronically cold temperatures and other extreme environmental conditions reduce the rates of biological processes, including the biodegradation of pollutants. However, the native Antarctic microbial diversity provides a reservoir of cold‐adapted microorganisms, some of which have the potential for biodegradation. This study evaluated the diesel hydrocarbon‐degrading ability of a psy-chrotolerant marine bacterial consortium obtained from the coast of the north‐west Antarctic Pen-insula. The consortium’s growth conditions were optimised using one‐factor‐at‐a‐time (OFAT) and statistical response surface methodology (RSM), which identified optimal growth conditions of pH 8.0, 10 °C, 25 ppt NaCl and 1.5 g/L NH4NO3. The predicted model was highly significant and con-firmed that the parameters’ salinity, temperature, nitrogen concentration and initial diesel concentration significantly influenced diesel biodegradation. Using the optimised values generated by RSM, a mass reduction of 12.23 mg/mL from the initial 30.518 mg/mL (4% (w/v)) concentration of diesel was achieved within a 6 d incubation period. This study provides further evidence for the presence of native hydrocarbon‐degrading bacteria in non‐contaminated Antarctic seawater.

Original languageEnglish
Article number1213
JournalMicroorganisms
Volume9
Issue number6
DOIs
Publication statusPublished - 2021 Jun

Keywords

  • Antarctica
  • Biodegradation
  • Diesel
  • Microbial consortium
  • Seawater

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)
  • Virology

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