Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus

Makoto Hisamatsu, Syuhei Nomura, Arawan Shutsrirung, Hitoshi Obata, Katsunori Teranishi, Tetsuya Yamada, Sukma Nuswantara, Mitsuo Yamashita, Yoshikatsu Murooka

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14 Citations (Scopus)

Abstract

Rhizobium huakuii isolated from root nodules of Astragalus sinicus produced a new acidic exopolysaccharide composed of glucose, galactose, ribose, and glucuronic acid in an approximate molar ratio of 5: 1: 1: 1. Degradation with an enzyme capable of specifically hydrolyzing succinoglycan and methylation analyses suggested that the main chain of the new exopolysaccharide has a common structure with acidic exopolysaccharides from Agrobacterium sp. and Rhizobium meliloti. The distribution pattern of the degrees of polymerization in cyclic (1→2) β-glucans of R. huakuii was more similar to that of R. meliloti than that of Agrobacterium sp.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalJournal of Fermentation and Bioengineering
Volume83
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

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Keywords

  • Depolymerase
  • Exopolysaccharide
  • Rhizobium huakuii
  • Ribose

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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