Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus

Makoto Hisamatsu; Syuhei Nomura; Arawan Shutsrirung; Hitoshi Obata; Katsunori Teranishi; Tetsuya Yamada; Sukma Nuswantara; Mitsuo Yamashita; Yoshikatsu Murooka

doi:10.1016/S0922-338X(97)80135-3

Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus

Makoto Hisamatsu, Syuhei Nomura, Arawan Shutsrirung, Hitoshi Obata, Katsunori Teranishi, Tetsuya Yamada, Sukma Nuswantara, Mitsuo Yamashita, Yoshikatsu Murooka

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Rhizobium huakuii isolated from root nodules of Astragalus sinicus produced a new acidic exopolysaccharide composed of glucose, galactose, ribose, and glucuronic acid in an approximate molar ratio of 5:1:1:1. Degradation with an enzyme capable of specifically hydrolyzing succinoglycan and methylation analyses suggested that the main chain of the new exopolysaccharide has a common structure with acidic exopolysaccharides from Agrobacterium sp. and Rhizobium meliloti. The distribution pattern of the degrees of polymerization in cyclic (1→2) β-glucans of R. huakuii was more similar to that of R. meliloti than that of Agrobacterium sp.

Original language	English
Pages (from-to)	315-320
Number of pages	6
Journal	Journal of Fermentation and Bioengineering
Volume	83
Issue number	4
DOIs	https://doi.org/10.1016/S0922-338X(97)80135-3
Publication status	Published - 1997
Externally published	Yes

Keywords

Depolymerase
Exopolysaccharide
Rhizobium huakuii
Ribose

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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10.1016/S0922-338X(97)80135-3

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Hisamatsu, M., Nomura, S., Shutsrirung, A., Obata, H., Teranishi, K., Yamada, T., Nuswantara, S., Yamashita, M., & Murooka, Y. (1997). Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus. Journal of Fermentation and Bioengineering, 83(4), 315-320. https://doi.org/10.1016/S0922-338X(97)80135-3

Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus. / Hisamatsu, Makoto; Nomura, Syuhei; Shutsrirung, Arawan; Obata, Hitoshi; Teranishi, Katsunori; Yamada, Tetsuya; Nuswantara, Sukma; Yamashita, Mitsuo; Murooka, Yoshikatsu.

In: Journal of Fermentation and Bioengineering, Vol. 83, No. 4, 1997, p. 315-320.

Research output: Contribution to journal › Article › peer-review

Hisamatsu, M, Nomura, S, Shutsrirung, A, Obata, H, Teranishi, K, Yamada, T, Nuswantara, S, Yamashita, M & Murooka, Y 1997, 'Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus', Journal of Fermentation and Bioengineering, vol. 83, no. 4, pp. 315-320. https://doi.org/10.1016/S0922-338X(97)80135-3

Hisamatsu, Makoto ; Nomura, Syuhei ; Shutsrirung, Arawan ; Obata, Hitoshi ; Teranishi, Katsunori ; Yamada, Tetsuya ; Nuswantara, Sukma ; Yamashita, Mitsuo ; Murooka, Yoshikatsu. / Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus. In: Journal of Fermentation and Bioengineering. 1997 ; Vol. 83, No. 4. pp. 315-320.

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title = "Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus",

abstract = "Rhizobium huakuii isolated from root nodules of Astragalus sinicus produced a new acidic exopolysaccharide composed of glucose, galactose, ribose, and glucuronic acid in an approximate molar ratio of 5:1:1:1. Degradation with an enzyme capable of specifically hydrolyzing succinoglycan and methylation analyses suggested that the main chain of the new exopolysaccharide has a common structure with acidic exopolysaccharides from Agrobacterium sp. and Rhizobium meliloti. The distribution pattern of the degrees of polymerization in cyclic (1→2) β-glucans of R. huakuii was more similar to that of R. meliloti than that of Agrobacterium sp.",

keywords = "Depolymerase, Exopolysaccharide, Rhizobium huakuii, Ribose",

author = "Makoto Hisamatsu and Syuhei Nomura and Arawan Shutsrirung and Hitoshi Obata and Katsunori Teranishi and Tetsuya Yamada and Sukma Nuswantara and Mitsuo Yamashita and Yoshikatsu Murooka",

note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research (No. 0845538) to Y. Murooka from the Ministry of Education, Science, Sports and Culture, Japan. A. Shutsrirung and S. Nuswantara were supported by the Japan International Cooperation Agency (JICA) and a Monbusho Student Scholarship, respectively.",

year = "1997",

doi = "10.1016/S0922-338X(97)80135-3",

language = "English",

volume = "83",

pages = "315--320",

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AU - Hisamatsu, Makoto

AU - Nomura, Syuhei

AU - Shutsrirung, Arawan

AU - Obata, Hitoshi

AU - Teranishi, Katsunori

AU - Yamada, Tetsuya

AU - Nuswantara, Sukma

AU - Yamashita, Mitsuo

AU - Murooka, Yoshikatsu

N1 - Funding Information: This work was supported by a Grant-in-Aid for Scientific Research (No. 0845538) to Y. Murooka from the Ministry of Education, Science, Sports and Culture, Japan. A. Shutsrirung and S. Nuswantara were supported by the Japan International Cooperation Agency (JICA) and a Monbusho Student Scholarship, respectively.

PY - 1997

Y1 - 1997

N2 - Rhizobium huakuii isolated from root nodules of Astragalus sinicus produced a new acidic exopolysaccharide composed of glucose, galactose, ribose, and glucuronic acid in an approximate molar ratio of 5:1:1:1. Degradation with an enzyme capable of specifically hydrolyzing succinoglycan and methylation analyses suggested that the main chain of the new exopolysaccharide has a common structure with acidic exopolysaccharides from Agrobacterium sp. and Rhizobium meliloti. The distribution pattern of the degrees of polymerization in cyclic (1→2) β-glucans of R. huakuii was more similar to that of R. meliloti than that of Agrobacterium sp.

AB - Rhizobium huakuii isolated from root nodules of Astragalus sinicus produced a new acidic exopolysaccharide composed of glucose, galactose, ribose, and glucuronic acid in an approximate molar ratio of 5:1:1:1. Degradation with an enzyme capable of specifically hydrolyzing succinoglycan and methylation analyses suggested that the main chain of the new exopolysaccharide has a common structure with acidic exopolysaccharides from Agrobacterium sp. and Rhizobium meliloti. The distribution pattern of the degrees of polymerization in cyclic (1→2) β-glucans of R. huakuii was more similar to that of R. meliloti than that of Agrobacterium sp.

KW - Depolymerase

KW - Exopolysaccharide

KW - Rhizobium huakuii

KW - Ribose

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Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus

Abstract

Keywords

ASJC Scopus subject areas

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