Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus

Makoto Hisamatsu, Syuhei Nomura, Arawan Shutsrirung, Hitoshi Obata, Katsunori Teranishi, Tetsuya Yamada, Sukma Nuswantara, Mitsuo Yamashita, Yoshikatsu Murooka

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Rhizobium huakuii isolated from root nodules of Astragalus sinicus produced a new acidic exopolysaccharide composed of glucose, galactose, ribose, and glucuronic acid in an approximate molar ratio of 5: 1: 1: 1. Degradation with an enzyme capable of specifically hydrolyzing succinoglycan and methylation analyses suggested that the main chain of the new exopolysaccharide has a common structure with acidic exopolysaccharides from Agrobacterium sp. and Rhizobium meliloti. The distribution pattern of the degrees of polymerization in cyclic (1→2) β-glucans of R. huakuii was more similar to that of R. meliloti than that of Agrobacterium sp.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalJournal of Fermentation and Bioengineering
Volume83
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Glucuronic Acid
Methylation
Ribose
Glucans
Galactose
Glucose
Enzymes
Polymerization
Degradation
Acids
succinoglycan

Keywords

  • Depolymerase
  • Exopolysaccharide
  • Rhizobium huakuii
  • Ribose

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus. / Hisamatsu, Makoto; Nomura, Syuhei; Shutsrirung, Arawan; Obata, Hitoshi; Teranishi, Katsunori; Yamada, Tetsuya; Nuswantara, Sukma; Yamashita, Mitsuo; Murooka, Yoshikatsu.

In: Journal of Fermentation and Bioengineering, Vol. 83, No. 4, 1997, p. 315-320.

Research output: Contribution to journalArticle

Hisamatsu, Makoto ; Nomura, Syuhei ; Shutsrirung, Arawan ; Obata, Hitoshi ; Teranishi, Katsunori ; Yamada, Tetsuya ; Nuswantara, Sukma ; Yamashita, Mitsuo ; Murooka, Yoshikatsu. / Structural characterization of a new acidic exopolysaccharide and cyclic (1→2)β-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus. In: Journal of Fermentation and Bioengineering. 1997 ; Vol. 83, No. 4. pp. 315-320.
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AU - Hisamatsu, Makoto

AU - Nomura, Syuhei

AU - Shutsrirung, Arawan

AU - Obata, Hitoshi

AU - Teranishi, Katsunori

AU - Yamada, Tetsuya

AU - Nuswantara, Sukma

AU - Yamashita, Mitsuo

AU - Murooka, Yoshikatsu

PY - 1997

Y1 - 1997

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AB - Rhizobium huakuii isolated from root nodules of Astragalus sinicus produced a new acidic exopolysaccharide composed of glucose, galactose, ribose, and glucuronic acid in an approximate molar ratio of 5: 1: 1: 1. Degradation with an enzyme capable of specifically hydrolyzing succinoglycan and methylation analyses suggested that the main chain of the new exopolysaccharide has a common structure with acidic exopolysaccharides from Agrobacterium sp. and Rhizobium meliloti. The distribution pattern of the degrees of polymerization in cyclic (1→2) β-glucans of R. huakuii was more similar to that of R. meliloti than that of Agrobacterium sp.

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