Structure and biosensor characteristics of complex between glucose oxidase and plasma-polymerized nanothin film

Hitoshi Muguruma, Yoshihiro Kase

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The structure and biosensor characteristics of complex between glucose oxidase (GOD) and plasma-polymerized nanothin film (PPF), in which the thickness is several nanometers, were investigated by atomic force microscopy (AFM) and electrochemical measurement. The GOD molecules were densely adsorbed onto the PPF surface treated by nitrogen plasma and the individual GOD molecules were observed. Subsequently, the GOD densely packed array on the PPF surface was subsequently treated by plasma polymerization (overcoating). AFM image showed that the thicker film gave the smoother surface, indicating that the GOD adsorbed on the surface was embedded more deeply in PPF. The amperometric biosensor characteristics of the GOD-PPF complex on a platinum electrode showed the current increment due to the enzymatic reaction with glucose addition, indicating that enzyme activity was retained although the enzyme has been exposed to the plasma gas related to diffusion of the substrate. This means that under mild exposure to organic plasma, the enzyme does not become seriously dysfunctional. Amperometric biosensor characteristics were strongly affected by monomer and thickness of PPF overcoating related with the diffusion of the substrate (glucose). Considering that the film deposition was performed using microfabrication-compatible organic plasma, our new method for protein architecture has a great potential of enabling high throughput production of bioelectronic devices.

Original languageEnglish
Pages (from-to)737-743
Number of pages7
JournalBiosensors and Bioelectronics
Volume22
Issue number5
DOIs
Publication statusPublished - 2006 Dec 15

Fingerprint

Glucose Oxidase
Glucose oxidase
Biosensors
Plasmas
Atomic force microscopy
Glucose
Plasma Gases
Enzymes
Nitrogen plasma
Plasma polymerization
Molecules
Microfabrication
Enzyme activity
Substrates
Platinum
Thick films
Monomers
Throughput
Electrodes
Proteins

Keywords

  • Amperometric biosensor
  • Glucose oxidase
  • Plasma-polymerized film
  • Protein architecture

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Structure and biosensor characteristics of complex between glucose oxidase and plasma-polymerized nanothin film. / Muguruma, Hitoshi; Kase, Yoshihiro.

In: Biosensors and Bioelectronics, Vol. 22, No. 5, 15.12.2006, p. 737-743.

Research output: Contribution to journalArticle

@article{dfa51b94addb484bb10624125f303ce3,
title = "Structure and biosensor characteristics of complex between glucose oxidase and plasma-polymerized nanothin film",
abstract = "The structure and biosensor characteristics of complex between glucose oxidase (GOD) and plasma-polymerized nanothin film (PPF), in which the thickness is several nanometers, were investigated by atomic force microscopy (AFM) and electrochemical measurement. The GOD molecules were densely adsorbed onto the PPF surface treated by nitrogen plasma and the individual GOD molecules were observed. Subsequently, the GOD densely packed array on the PPF surface was subsequently treated by plasma polymerization (overcoating). AFM image showed that the thicker film gave the smoother surface, indicating that the GOD adsorbed on the surface was embedded more deeply in PPF. The amperometric biosensor characteristics of the GOD-PPF complex on a platinum electrode showed the current increment due to the enzymatic reaction with glucose addition, indicating that enzyme activity was retained although the enzyme has been exposed to the plasma gas related to diffusion of the substrate. This means that under mild exposure to organic plasma, the enzyme does not become seriously dysfunctional. Amperometric biosensor characteristics were strongly affected by monomer and thickness of PPF overcoating related with the diffusion of the substrate (glucose). Considering that the film deposition was performed using microfabrication-compatible organic plasma, our new method for protein architecture has a great potential of enabling high throughput production of bioelectronic devices.",
keywords = "Amperometric biosensor, Glucose oxidase, Plasma-polymerized film, Protein architecture",
author = "Hitoshi Muguruma and Yoshihiro Kase",
year = "2006",
month = "12",
day = "15",
doi = "10.1016/j.bios.2006.02.017",
language = "English",
volume = "22",
pages = "737--743",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Structure and biosensor characteristics of complex between glucose oxidase and plasma-polymerized nanothin film

AU - Muguruma, Hitoshi

AU - Kase, Yoshihiro

PY - 2006/12/15

Y1 - 2006/12/15

N2 - The structure and biosensor characteristics of complex between glucose oxidase (GOD) and plasma-polymerized nanothin film (PPF), in which the thickness is several nanometers, were investigated by atomic force microscopy (AFM) and electrochemical measurement. The GOD molecules were densely adsorbed onto the PPF surface treated by nitrogen plasma and the individual GOD molecules were observed. Subsequently, the GOD densely packed array on the PPF surface was subsequently treated by plasma polymerization (overcoating). AFM image showed that the thicker film gave the smoother surface, indicating that the GOD adsorbed on the surface was embedded more deeply in PPF. The amperometric biosensor characteristics of the GOD-PPF complex on a platinum electrode showed the current increment due to the enzymatic reaction with glucose addition, indicating that enzyme activity was retained although the enzyme has been exposed to the plasma gas related to diffusion of the substrate. This means that under mild exposure to organic plasma, the enzyme does not become seriously dysfunctional. Amperometric biosensor characteristics were strongly affected by monomer and thickness of PPF overcoating related with the diffusion of the substrate (glucose). Considering that the film deposition was performed using microfabrication-compatible organic plasma, our new method for protein architecture has a great potential of enabling high throughput production of bioelectronic devices.

AB - The structure and biosensor characteristics of complex between glucose oxidase (GOD) and plasma-polymerized nanothin film (PPF), in which the thickness is several nanometers, were investigated by atomic force microscopy (AFM) and electrochemical measurement. The GOD molecules were densely adsorbed onto the PPF surface treated by nitrogen plasma and the individual GOD molecules were observed. Subsequently, the GOD densely packed array on the PPF surface was subsequently treated by plasma polymerization (overcoating). AFM image showed that the thicker film gave the smoother surface, indicating that the GOD adsorbed on the surface was embedded more deeply in PPF. The amperometric biosensor characteristics of the GOD-PPF complex on a platinum electrode showed the current increment due to the enzymatic reaction with glucose addition, indicating that enzyme activity was retained although the enzyme has been exposed to the plasma gas related to diffusion of the substrate. This means that under mild exposure to organic plasma, the enzyme does not become seriously dysfunctional. Amperometric biosensor characteristics were strongly affected by monomer and thickness of PPF overcoating related with the diffusion of the substrate (glucose). Considering that the film deposition was performed using microfabrication-compatible organic plasma, our new method for protein architecture has a great potential of enabling high throughput production of bioelectronic devices.

KW - Amperometric biosensor

KW - Glucose oxidase

KW - Plasma-polymerized film

KW - Protein architecture

UR - http://www.scopus.com/inward/record.url?scp=33750322851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750322851&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2006.02.017

DO - 10.1016/j.bios.2006.02.017

M3 - Article

C2 - 16600587

AN - SCOPUS:33750322851

VL - 22

SP - 737

EP - 743

JO - Biosensors

JF - Biosensors

SN - 0956-5663

IS - 5

ER -