Structure of ige epitopes on a new 39-KD allergen molecule from the house dust mite, dermatophagoides farina

Tsunehiro Aki; Kazuhisa Ono; Yuuji Hidaka; Yasutsugu Shimonishi; Toshihiko Jyo; Takeshi Wada; Mitsuo Yamashita; Seiko Shigeta; Yoshikatsu Murooka; Satoru Oka

doi:10.1159/000236654

Structure of ige epitopes on a new 39-KD allergen molecule from the house dust mite, dermatophagoides farina

Tsunehiro Aki, Kazuhisa Ono, Yuuji Hidaka, Yasutsugu Shimonishi, Toshihiko Jyo, Takeshi Wada, Mitsuo Yamashita, Seiko Shigeta, Yoshikatsu Murooka, Satoru Oka

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Two IgE epitope sequences comprising Ser⁵⁶-Pro-Val-Thr-Lys-Arg-Ala-Ser- Leu-Lys-Ile-Asp-Ser-Lys-Lys⁷⁰ and Asp¹⁰⁴-Val-GIu-Leu-Ser-Leu-Arg-Ser-Ser-Asp-Ile-Ala¹¹⁵ were identified by deletion analysis of the cDNA encoding a new 39-kD protein of mite allergen. A syntheticdodecapeptide corresponding to the latter epitope sequence functioned as a monovalent and mite-specific hapten. Replacement of each of the 12 amino acid residues with Gly, using site-directed mutagenesis, indicated that Arg¹¹⁰ may play a central role in IgE binding. However, the 8 allergic sera tested exhibited a wide variation in their amino acid residues required for reactivity to IgE in allergic sera.

Original language	English
Pages (from-to)	357-364
Number of pages	8
Journal	International Archives of Allergy and Immunology
Volume	103
Issue number	4
DOIs	https://doi.org/10.1159/000236654
Publication status	Published - 1994
Externally published	Yes

Keywords

Dermatophagoides farina
Epitope mapping
IgE epitope
Mite allergen

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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Structure of ige epitopes on a new 39-KD allergen molecule from the house dust mite, dermatophagoides farina. / Aki, Tsunehiro; Ono, Kazuhisa; Hidaka, Yuuji; Shimonishi, Yasutsugu; Jyo, Toshihiko; Wada, Takeshi; Yamashita, Mitsuo; Shigeta, Seiko; Murooka, Yoshikatsu; Oka, Satoru.

In: International Archives of Allergy and Immunology, Vol. 103, No. 4, 1994, p. 357-364.

Research output: Contribution to journal › Article › peer-review

Aki, Tsunehiro ; Ono, Kazuhisa ; Hidaka, Yuuji ; Shimonishi, Yasutsugu ; Jyo, Toshihiko ; Wada, Takeshi ; Yamashita, Mitsuo ; Shigeta, Seiko ; Murooka, Yoshikatsu ; Oka, Satoru. / Structure of ige epitopes on a new 39-KD allergen molecule from the house dust mite, dermatophagoides farina. In: International Archives of Allergy and Immunology. 1994 ; Vol. 103, No. 4. pp. 357-364.

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abstract = "Two IgE epitope sequences comprising Ser56-Pro-Val-Thr-Lys-Arg-Ala-Ser- Leu-Lys-Ile-Asp-Ser-Lys-Lys70 and Asp104-Val-GIu-Leu-Ser-Leu-Arg-Ser-Ser-Asp-Ile-Ala115 were identified by deletion analysis of the cDNA encoding a new 39-kD protein of mite allergen. A syntheticdodecapeptide corresponding to the latter epitope sequence functioned as a monovalent and mite-specific hapten. Replacement of each of the 12 amino acid residues with Gly, using site-directed mutagenesis, indicated that Arg110 may play a central role in IgE binding. However, the 8 allergic sera tested exhibited a wide variation in their amino acid residues required for reactivity to IgE in allergic sera.",

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AU - Jyo, Toshihiko

AU - Wada, Takeshi

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