Structure of IgE epitopes on a new 39-kD allergen molecule from the house dust mite, Dermatophagoides farinae

T. Aki, K. Ono, Y. Hidaka, Y. Shimonishi, T. Jyo, T. Wada, Mitsuo Yamashita, S. Shigeta, Y. Murooka, S. Oka

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Two IgE epitope sequences comprising Ser56-Pro-Val-Thr-Lys-Arg-Ala-Ser-Leu-Lys-Ile-Asp-Ser-Lys-Lys70 and Asp104-Val-Glu-Leu-Ser-Leu-Arg-Ser-Ser-Asp-Ile-Ala115 were identified by deletion analysis of the cDNA encoding a new 39-kD protein of mite allergen. A synthetic dodecapeptide corresponding to the latter epitope sequence functioned as a monovalent and mite-specific hapten. Replacement of each of the 12 amino acid residues with Gly, using site-directed mutagenesis, indicated that Arg110 may play a central role in IgE binding. However, the 8 allergic sera tested exhibited a wide variation in their amino acid residues required for reactivity to IgE in allergic sera.

Original languageEnglish
Pages (from-to)357-364
Number of pages8
JournalInternational Archives of Allergy and Immunology
Volume103
Issue number4
Publication statusPublished - 1994
Externally publishedYes

Keywords

  • Dermatophagoides farinae
  • Epitope mapping
  • IgE epitope
  • Mite allergen

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Aki, T., Ono, K., Hidaka, Y., Shimonishi, Y., Jyo, T., Wada, T., ... Oka, S. (1994). Structure of IgE epitopes on a new 39-kD allergen molecule from the house dust mite, Dermatophagoides farinae. International Archives of Allergy and Immunology, 103(4), 357-364.

Structure of IgE epitopes on a new 39-kD allergen molecule from the house dust mite, Dermatophagoides farinae. / Aki, T.; Ono, K.; Hidaka, Y.; Shimonishi, Y.; Jyo, T.; Wada, T.; Yamashita, Mitsuo; Shigeta, S.; Murooka, Y.; Oka, S.

In: International Archives of Allergy and Immunology, Vol. 103, No. 4, 1994, p. 357-364.

Research output: Contribution to journalArticle

Aki, T, Ono, K, Hidaka, Y, Shimonishi, Y, Jyo, T, Wada, T, Yamashita, M, Shigeta, S, Murooka, Y & Oka, S 1994, 'Structure of IgE epitopes on a new 39-kD allergen molecule from the house dust mite, Dermatophagoides farinae', International Archives of Allergy and Immunology, vol. 103, no. 4, pp. 357-364.
Aki, T. ; Ono, K. ; Hidaka, Y. ; Shimonishi, Y. ; Jyo, T. ; Wada, T. ; Yamashita, Mitsuo ; Shigeta, S. ; Murooka, Y. ; Oka, S. / Structure of IgE epitopes on a new 39-kD allergen molecule from the house dust mite, Dermatophagoides farinae. In: International Archives of Allergy and Immunology. 1994 ; Vol. 103, No. 4. pp. 357-364.
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AU - Wada, T.

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