Suppressive effects of cacao liquor polyphenols (CLP) on LDL oxidation and the development of atherosclerosis in Kurosawa and Kusanagi-hypercholesterolemic rabbits

Tohru Kurosawa, Fumi Itoh, Aiko Nozaki, Yoshihisa Nakano, Shin Ichiro Katsuda, Naomi Osakabe, Hirokazu Tsubone, Kazuo Kondo, Hiroshige Itakura

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

We investigated the properties of cacao liquor polyphenols (CLP), which have an antioxidative effect on low-density lipoprotein (LDL) and an anti-atherosclerotic effect in the spontaneous familial hypercholesterolemic model, the Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbit. After 6 months of dietary administration of CLP at 1% (w/w) to the KHC rabbits, a higher total cholesterol concentration was observed in the treatment group compared to the control group. However, no other effects were noted in lipid profiles in plasma or lipoproteins. The plasma concentration of thiobarbituric acid reactive substances (TBARS), which is a lipid-peroxidation index, was significantly decreased 1 month after the start of CLP administration compared to that of the control group. The antioxidative effect of CLP on LDL was observed from 2 to 4 months of administration. The area of atherosclerotic lesions in the aorta in the CLP group (32.01 ± 1.58%) was significantly smaller than that in the control group (47.05 ± 3.29%), and the tissue cholesterol and TBARS concentrations were lower in the CLP group than in the control group. The anti-atherosclerotic effect of CLP was confirmed both rheologically and histopathologically. An in vitro study using KHC rabbit-derived LDL revealed that CLP significantly prolonged the lag time of LDL oxidation that was induced by a lipophilic azo-radical initiator, 2,2′-azobis(4-methoxy)-2,4- dimethylvaleronitrile (V-70), or Cu2+ from a low concentration of 0.1 μg/mL. The antioxidative effect of CLP was superior to those of the well-known antioxidative substances, vitamin C, vitamin E and probucol. Therefore, CLP suppressed the generation of atherosclerosis, and its antioxidative effect appeared to have an important role in its anti-atherosclerotic activity.

Original languageEnglish
Pages (from-to)237-246
Number of pages10
JournalAtherosclerosis
Volume179
Issue number2
DOIs
Publication statusPublished - 2005 Apr
Externally publishedYes

Keywords

  • Antioxidation
  • Atherosclerosis
  • Cacao liquor polyphenols
  • Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbit

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Suppressive effects of cacao liquor polyphenols (CLP) on LDL oxidation and the development of atherosclerosis in Kurosawa and Kusanagi-hypercholesterolemic rabbits. / Kurosawa, Tohru; Itoh, Fumi; Nozaki, Aiko; Nakano, Yoshihisa; Katsuda, Shin Ichiro; Osakabe, Naomi; Tsubone, Hirokazu; Kondo, Kazuo; Itakura, Hiroshige.

In: Atherosclerosis, Vol. 179, No. 2, 04.2005, p. 237-246.

Research output: Contribution to journalArticle

Kurosawa, Tohru ; Itoh, Fumi ; Nozaki, Aiko ; Nakano, Yoshihisa ; Katsuda, Shin Ichiro ; Osakabe, Naomi ; Tsubone, Hirokazu ; Kondo, Kazuo ; Itakura, Hiroshige. / Suppressive effects of cacao liquor polyphenols (CLP) on LDL oxidation and the development of atherosclerosis in Kurosawa and Kusanagi-hypercholesterolemic rabbits. In: Atherosclerosis. 2005 ; Vol. 179, No. 2. pp. 237-246.
@article{790af0013bd44ade9a5029fa1747cb71,
title = "Suppressive effects of cacao liquor polyphenols (CLP) on LDL oxidation and the development of atherosclerosis in Kurosawa and Kusanagi-hypercholesterolemic rabbits",
abstract = "We investigated the properties of cacao liquor polyphenols (CLP), which have an antioxidative effect on low-density lipoprotein (LDL) and an anti-atherosclerotic effect in the spontaneous familial hypercholesterolemic model, the Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbit. After 6 months of dietary administration of CLP at 1{\%} (w/w) to the KHC rabbits, a higher total cholesterol concentration was observed in the treatment group compared to the control group. However, no other effects were noted in lipid profiles in plasma or lipoproteins. The plasma concentration of thiobarbituric acid reactive substances (TBARS), which is a lipid-peroxidation index, was significantly decreased 1 month after the start of CLP administration compared to that of the control group. The antioxidative effect of CLP on LDL was observed from 2 to 4 months of administration. The area of atherosclerotic lesions in the aorta in the CLP group (32.01 ± 1.58{\%}) was significantly smaller than that in the control group (47.05 ± 3.29{\%}), and the tissue cholesterol and TBARS concentrations were lower in the CLP group than in the control group. The anti-atherosclerotic effect of CLP was confirmed both rheologically and histopathologically. An in vitro study using KHC rabbit-derived LDL revealed that CLP significantly prolonged the lag time of LDL oxidation that was induced by a lipophilic azo-radical initiator, 2,2′-azobis(4-methoxy)-2,4- dimethylvaleronitrile (V-70), or Cu2+ from a low concentration of 0.1 μg/mL. The antioxidative effect of CLP was superior to those of the well-known antioxidative substances, vitamin C, vitamin E and probucol. Therefore, CLP suppressed the generation of atherosclerosis, and its antioxidative effect appeared to have an important role in its anti-atherosclerotic activity.",
keywords = "Antioxidation, Atherosclerosis, Cacao liquor polyphenols, Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbit",
author = "Tohru Kurosawa and Fumi Itoh and Aiko Nozaki and Yoshihisa Nakano and Katsuda, {Shin Ichiro} and Naomi Osakabe and Hirokazu Tsubone and Kazuo Kondo and Hiroshige Itakura",
year = "2005",
month = "4",
doi = "10.1016/j.atherosclerosis.2004.12.003",
language = "English",
volume = "179",
pages = "237--246",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Suppressive effects of cacao liquor polyphenols (CLP) on LDL oxidation and the development of atherosclerosis in Kurosawa and Kusanagi-hypercholesterolemic rabbits

AU - Kurosawa, Tohru

AU - Itoh, Fumi

AU - Nozaki, Aiko

AU - Nakano, Yoshihisa

AU - Katsuda, Shin Ichiro

AU - Osakabe, Naomi

AU - Tsubone, Hirokazu

AU - Kondo, Kazuo

AU - Itakura, Hiroshige

PY - 2005/4

Y1 - 2005/4

N2 - We investigated the properties of cacao liquor polyphenols (CLP), which have an antioxidative effect on low-density lipoprotein (LDL) and an anti-atherosclerotic effect in the spontaneous familial hypercholesterolemic model, the Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbit. After 6 months of dietary administration of CLP at 1% (w/w) to the KHC rabbits, a higher total cholesterol concentration was observed in the treatment group compared to the control group. However, no other effects were noted in lipid profiles in plasma or lipoproteins. The plasma concentration of thiobarbituric acid reactive substances (TBARS), which is a lipid-peroxidation index, was significantly decreased 1 month after the start of CLP administration compared to that of the control group. The antioxidative effect of CLP on LDL was observed from 2 to 4 months of administration. The area of atherosclerotic lesions in the aorta in the CLP group (32.01 ± 1.58%) was significantly smaller than that in the control group (47.05 ± 3.29%), and the tissue cholesterol and TBARS concentrations were lower in the CLP group than in the control group. The anti-atherosclerotic effect of CLP was confirmed both rheologically and histopathologically. An in vitro study using KHC rabbit-derived LDL revealed that CLP significantly prolonged the lag time of LDL oxidation that was induced by a lipophilic azo-radical initiator, 2,2′-azobis(4-methoxy)-2,4- dimethylvaleronitrile (V-70), or Cu2+ from a low concentration of 0.1 μg/mL. The antioxidative effect of CLP was superior to those of the well-known antioxidative substances, vitamin C, vitamin E and probucol. Therefore, CLP suppressed the generation of atherosclerosis, and its antioxidative effect appeared to have an important role in its anti-atherosclerotic activity.

AB - We investigated the properties of cacao liquor polyphenols (CLP), which have an antioxidative effect on low-density lipoprotein (LDL) and an anti-atherosclerotic effect in the spontaneous familial hypercholesterolemic model, the Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbit. After 6 months of dietary administration of CLP at 1% (w/w) to the KHC rabbits, a higher total cholesterol concentration was observed in the treatment group compared to the control group. However, no other effects were noted in lipid profiles in plasma or lipoproteins. The plasma concentration of thiobarbituric acid reactive substances (TBARS), which is a lipid-peroxidation index, was significantly decreased 1 month after the start of CLP administration compared to that of the control group. The antioxidative effect of CLP on LDL was observed from 2 to 4 months of administration. The area of atherosclerotic lesions in the aorta in the CLP group (32.01 ± 1.58%) was significantly smaller than that in the control group (47.05 ± 3.29%), and the tissue cholesterol and TBARS concentrations were lower in the CLP group than in the control group. The anti-atherosclerotic effect of CLP was confirmed both rheologically and histopathologically. An in vitro study using KHC rabbit-derived LDL revealed that CLP significantly prolonged the lag time of LDL oxidation that was induced by a lipophilic azo-radical initiator, 2,2′-azobis(4-methoxy)-2,4- dimethylvaleronitrile (V-70), or Cu2+ from a low concentration of 0.1 μg/mL. The antioxidative effect of CLP was superior to those of the well-known antioxidative substances, vitamin C, vitamin E and probucol. Therefore, CLP suppressed the generation of atherosclerosis, and its antioxidative effect appeared to have an important role in its anti-atherosclerotic activity.

KW - Antioxidation

KW - Atherosclerosis

KW - Cacao liquor polyphenols

KW - Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbit

UR - http://www.scopus.com/inward/record.url?scp=14944342984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14944342984&partnerID=8YFLogxK

U2 - 10.1016/j.atherosclerosis.2004.12.003

DO - 10.1016/j.atherosclerosis.2004.12.003

M3 - Article

C2 - 15777537

AN - SCOPUS:14944342984

VL - 179

SP - 237

EP - 246

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

IS - 2

ER -