Surface plasmon resonance imaging of Au nano-partifcle modified DNA monolayers

Akira Yamaguchi; Masayuki Hayashida; Saulius Juodkazis; Yoichiro Hara; Shigeki Matsuo; Hiroaki Misawa

doi:10.1117/12.491216

Surface plasmon resonance imaging of Au nano-partifcle modified DNA monolayers

Akira Yamaguchi, Masayuki Hayashida, Saulius Juodkazis, Yoichiro Hara, Shigeki Matsuo, Hiroaki Misawa

Research output: Contribution to journal › Conference article › peer-review

Abstract

A probe DNA monolayer modified with Au nano-particles was prepared on an Au thin film and used for DNA hybridization detection by surface plasmon resonance (SPR) spectroscopy. This Au particle modified probe DNA monolayer resulted in about a 3.5 times larger SPR angle shift according to the hybridization reaction, compared to that found in a non-modified probe DNA monolayer. By comparison of the SPR image of the particle modified and non-modified probe DNA monolayer, this modification method was shown to be useful in improving the SPR signal for the detection of the unlabelled DNA molecules.

Original language	English
Pages (from-to)	247-251
Number of pages	5
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4626
DOIs	https://doi.org/10.1117/12.491216
Publication status	Published - 2002
Externally published	Yes
Event	Biomedical Nanotechnology Architectures and Applications - San Jose, CA, United States Duration: 2002 Jan 20 → 2002 Jan 24

Keywords

AFM
Au nano-particle
DNA
Hybridization
SPR

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.491216

Cite this

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title = "Surface plasmon resonance imaging of Au nano-partifcle modified DNA monolayers",

abstract = "A probe DNA monolayer modified with Au nano-particles was prepared on an Au thin film and used for DNA hybridization detection by surface plasmon resonance (SPR) spectroscopy. This Au particle modified probe DNA monolayer resulted in about a 3.5 times larger SPR angle shift according to the hybridization reaction, compared to that found in a non-modified probe DNA monolayer. By comparison of the SPR image of the particle modified and non-modified probe DNA monolayer, this modification method was shown to be useful in improving the SPR signal for the detection of the unlabelled DNA molecules.",

keywords = "AFM, Au nano-particle, DNA, Hybridization, SPR",

author = "Akira Yamaguchi and Masayuki Hayashida and Saulius Juodkazis and Yoichiro Hara and Shigeki Matsuo and Hiroaki Misawa",

year = "2002",

doi = "10.1117/12.491216",

language = "English",

volume = "4626",

pages = "247--251",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

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TY - JOUR

T1 - Surface plasmon resonance imaging of Au nano-partifcle modified DNA monolayers

AU - Yamaguchi, Akira

AU - Hayashida, Masayuki

AU - Juodkazis, Saulius

AU - Hara, Yoichiro

AU - Matsuo, Shigeki

AU - Misawa, Hiroaki

PY - 2002

Y1 - 2002

N2 - A probe DNA monolayer modified with Au nano-particles was prepared on an Au thin film and used for DNA hybridization detection by surface plasmon resonance (SPR) spectroscopy. This Au particle modified probe DNA monolayer resulted in about a 3.5 times larger SPR angle shift according to the hybridization reaction, compared to that found in a non-modified probe DNA monolayer. By comparison of the SPR image of the particle modified and non-modified probe DNA monolayer, this modification method was shown to be useful in improving the SPR signal for the detection of the unlabelled DNA molecules.

AB - A probe DNA monolayer modified with Au nano-particles was prepared on an Au thin film and used for DNA hybridization detection by surface plasmon resonance (SPR) spectroscopy. This Au particle modified probe DNA monolayer resulted in about a 3.5 times larger SPR angle shift according to the hybridization reaction, compared to that found in a non-modified probe DNA monolayer. By comparison of the SPR image of the particle modified and non-modified probe DNA monolayer, this modification method was shown to be useful in improving the SPR signal for the detection of the unlabelled DNA molecules.

KW - AFM

KW - Au nano-particle

KW - DNA

KW - Hybridization

KW - SPR

UR - http://www.scopus.com/inward/record.url?scp=0036408223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036408223&partnerID=8YFLogxK

U2 - 10.1117/12.491216

DO - 10.1117/12.491216

M3 - Conference article

AN - SCOPUS:0036408223

SN - 0277-786X

VL - 4626

SP - 247

EP - 251

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Biomedical Nanotechnology Architectures and Applications

Y2 - 20 January 2002 through 24 January 2002

ER -

Surface plasmon resonance imaging of Au nano-partifcle modified DNA monolayers

Abstract

Keywords

ASJC Scopus subject areas

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