Surface plasmon resonance immunosensor for anionic surfactants based on an indirect competitive immunoreaction

Takashi Masadome, Yoshinori Yano, Toshihiko Imato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A highly sensitive surface plasmon resonance immunosensor for the determination of linear alkylbenzene sulfonate (LAS) was fabricated. The method is based on an indirect competitive reaction of an anti-LAS antibody in a sample solution with LAS immobilized on a sensor chip and with LAS in the sample solution. A sensor chip immobilized with LAS was prepared by utilizing an electrostatic interaction between an LAS conjugate, LAS-horseradish peroxidase (LAS-HRP), and a self-assembled monolayer of 11-amino-1-undecanethiol hydrochloride, which was preliminary prepared on a gold thin film of the sensor chip. The quantitative determination of LAS in the concentration range 10-1000 ppb was achieved by using the proposed immunosensor.

Original languageEnglish
Pages (from-to)640-648
Number of pages9
JournalAnalytical Letters
Volume41
Issue number4
DOIs
Publication statusPublished - 2008 Jan 1

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Keywords

  • Anionic surfactants
  • Indirect competitive immunosensor
  • LAS-horseradish peroxidase conjugate
  • Linear alkylbenzene sulfonate (LAS)
  • Self-assembled monolayer
  • Surface plasmon resonance (SPR)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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