Synthesis of Au Nanoparticles in Natural Matrices by Liquid-Phase Plasma: Effects on Cytotoxic Activity against Normal and Cancer Cell Lines

Nidar Treesukkasem, Chayanaphat Chokradjaroen, Sewan Theeramunkong, Nagahiro Saito, Anyarat Watthanaphanit

Research output: Contribution to journalArticle

Abstract

Wrapping of gold nanoparticles (AuNPs) with a biocompatible matrix, to gain AuNP colloids, is a general strategy to synthesize the AuNPs for biomedical purposes. This work reports the synthesis of AuNP colloids in the aqueous solution of several natural matrices using a liquid-phase plasma process. Two classes of natural substances used are sugars (including: glucose, fructose, and sucrose) and biopolymers (including: carboxymethyl cellulose, sodium alginate, and gelatin). All are negatively charged water-soluble substances that are claimed to be sources of energy for cells to grow and have a high potential to be compatible with them. The study has emerged since one question arises: "Do these matrices also promote the growth of cancer cells?" The synthesis is performed by generating plasma across a pair of electrodes immersed in an aqueous solution of a natural matrix containing a gold precursor (HAuCl4·3H2O). Two concentrations of the matrices (0.5 and 1.0% w/v) are used, and the plasma treatment times are varied (0, 10, and 30 min). The effect of the type and concentration of natural matrices as well as the plasma treatment time on the formation of AuNPs, along with their physical and chemical properties, including morphology, size, hydrodynamic diameter (dh), colloidal stability, and charge on the AuNP surface, is evaluated. We find that the charge of the AuNP surfaces could be altered by the plasma treatment. Eventually, cytotoxicity test results against normal (MRC-5) and cancer (H460 and HeLa) cell lines could not only answer our opening question but also suggest a rather complex response. Our findings indicate the great potential of the obtained AuNP colloids as a part of cancer therapy.

Original languageEnglish
JournalACS Applied Nano Materials
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Cells
Nanoparticles
Plasmas
Colloids
Liquids
Sugar (sucrose)
Gold
Sodium alginate
Carboxymethylcellulose Sodium
Fructose
Biopolymers
Gelatin
Cytotoxicity
Sugars
Chemical properties
Glucose
Sucrose
Cellulose
Hydrodynamics
Physical properties

Keywords

  • biopolymer
  • cancer
  • cytotoxicity
  • gold nanoparticle (AuNP)
  • liquid-phase plasma
  • sugar

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Synthesis of Au Nanoparticles in Natural Matrices by Liquid-Phase Plasma : Effects on Cytotoxic Activity against Normal and Cancer Cell Lines. / Treesukkasem, Nidar; Chokradjaroen, Chayanaphat; Theeramunkong, Sewan; Saito, Nagahiro; Watthanaphanit, Anyarat.

In: ACS Applied Nano Materials, 01.01.2019.

Research output: Contribution to journalArticle

@article{6eee298e9213444c8ca8acdee7f1df82,
title = "Synthesis of Au Nanoparticles in Natural Matrices by Liquid-Phase Plasma: Effects on Cytotoxic Activity against Normal and Cancer Cell Lines",
abstract = "Wrapping of gold nanoparticles (AuNPs) with a biocompatible matrix, to gain AuNP colloids, is a general strategy to synthesize the AuNPs for biomedical purposes. This work reports the synthesis of AuNP colloids in the aqueous solution of several natural matrices using a liquid-phase plasma process. Two classes of natural substances used are sugars (including: glucose, fructose, and sucrose) and biopolymers (including: carboxymethyl cellulose, sodium alginate, and gelatin). All are negatively charged water-soluble substances that are claimed to be sources of energy for cells to grow and have a high potential to be compatible with them. The study has emerged since one question arises: {"}Do these matrices also promote the growth of cancer cells?{"} The synthesis is performed by generating plasma across a pair of electrodes immersed in an aqueous solution of a natural matrix containing a gold precursor (HAuCl4·3H2O). Two concentrations of the matrices (0.5 and 1.0{\%} w/v) are used, and the plasma treatment times are varied (0, 10, and 30 min). The effect of the type and concentration of natural matrices as well as the plasma treatment time on the formation of AuNPs, along with their physical and chemical properties, including morphology, size, hydrodynamic diameter (dh), colloidal stability, and charge on the AuNP surface, is evaluated. We find that the charge of the AuNP surfaces could be altered by the plasma treatment. Eventually, cytotoxicity test results against normal (MRC-5) and cancer (H460 and HeLa) cell lines could not only answer our opening question but also suggest a rather complex response. Our findings indicate the great potential of the obtained AuNP colloids as a part of cancer therapy.",
keywords = "biopolymer, cancer, cytotoxicity, gold nanoparticle (AuNP), liquid-phase plasma, sugar",
author = "Nidar Treesukkasem and Chayanaphat Chokradjaroen and Sewan Theeramunkong and Nagahiro Saito and Anyarat Watthanaphanit",
year = "2019",
month = "1",
day = "1",
doi = "10.1021/acsanm.9b02106",
language = "English",
journal = "ACS Applied Nano Materials",
issn = "2574-0970",
publisher = "American Chemical Society",

}

TY - JOUR

T1 - Synthesis of Au Nanoparticles in Natural Matrices by Liquid-Phase Plasma

T2 - Effects on Cytotoxic Activity against Normal and Cancer Cell Lines

AU - Treesukkasem, Nidar

AU - Chokradjaroen, Chayanaphat

AU - Theeramunkong, Sewan

AU - Saito, Nagahiro

AU - Watthanaphanit, Anyarat

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Wrapping of gold nanoparticles (AuNPs) with a biocompatible matrix, to gain AuNP colloids, is a general strategy to synthesize the AuNPs for biomedical purposes. This work reports the synthesis of AuNP colloids in the aqueous solution of several natural matrices using a liquid-phase plasma process. Two classes of natural substances used are sugars (including: glucose, fructose, and sucrose) and biopolymers (including: carboxymethyl cellulose, sodium alginate, and gelatin). All are negatively charged water-soluble substances that are claimed to be sources of energy for cells to grow and have a high potential to be compatible with them. The study has emerged since one question arises: "Do these matrices also promote the growth of cancer cells?" The synthesis is performed by generating plasma across a pair of electrodes immersed in an aqueous solution of a natural matrix containing a gold precursor (HAuCl4·3H2O). Two concentrations of the matrices (0.5 and 1.0% w/v) are used, and the plasma treatment times are varied (0, 10, and 30 min). The effect of the type and concentration of natural matrices as well as the plasma treatment time on the formation of AuNPs, along with their physical and chemical properties, including morphology, size, hydrodynamic diameter (dh), colloidal stability, and charge on the AuNP surface, is evaluated. We find that the charge of the AuNP surfaces could be altered by the plasma treatment. Eventually, cytotoxicity test results against normal (MRC-5) and cancer (H460 and HeLa) cell lines could not only answer our opening question but also suggest a rather complex response. Our findings indicate the great potential of the obtained AuNP colloids as a part of cancer therapy.

AB - Wrapping of gold nanoparticles (AuNPs) with a biocompatible matrix, to gain AuNP colloids, is a general strategy to synthesize the AuNPs for biomedical purposes. This work reports the synthesis of AuNP colloids in the aqueous solution of several natural matrices using a liquid-phase plasma process. Two classes of natural substances used are sugars (including: glucose, fructose, and sucrose) and biopolymers (including: carboxymethyl cellulose, sodium alginate, and gelatin). All are negatively charged water-soluble substances that are claimed to be sources of energy for cells to grow and have a high potential to be compatible with them. The study has emerged since one question arises: "Do these matrices also promote the growth of cancer cells?" The synthesis is performed by generating plasma across a pair of electrodes immersed in an aqueous solution of a natural matrix containing a gold precursor (HAuCl4·3H2O). Two concentrations of the matrices (0.5 and 1.0% w/v) are used, and the plasma treatment times are varied (0, 10, and 30 min). The effect of the type and concentration of natural matrices as well as the plasma treatment time on the formation of AuNPs, along with their physical and chemical properties, including morphology, size, hydrodynamic diameter (dh), colloidal stability, and charge on the AuNP surface, is evaluated. We find that the charge of the AuNP surfaces could be altered by the plasma treatment. Eventually, cytotoxicity test results against normal (MRC-5) and cancer (H460 and HeLa) cell lines could not only answer our opening question but also suggest a rather complex response. Our findings indicate the great potential of the obtained AuNP colloids as a part of cancer therapy.

KW - biopolymer

KW - cancer

KW - cytotoxicity

KW - gold nanoparticle (AuNP)

KW - liquid-phase plasma

KW - sugar

UR - http://www.scopus.com/inward/record.url?scp=85075551118&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85075551118&partnerID=8YFLogxK

U2 - 10.1021/acsanm.9b02106

DO - 10.1021/acsanm.9b02106

M3 - Article

AN - SCOPUS:85075551118

JO - ACS Applied Nano Materials

JF - ACS Applied Nano Materials

SN - 2574-0970

ER -