The cutting edge of histochemical technique: In situ PCR

Yoshiki Hiraoka; Motoyuki Ogawa; Yukinao Sakai; Sadakazu Aiso

doi:10.1267/ahc.31.255

The cutting edge of histochemical technique: In situ PCR

Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Sadakazu Aiso

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The most innovative technique introduced recently into the field of biological study is the polymerase chain reaction (PCR) which can amplify even single-copy DNA sequences to high levels. In situ PCR, which is a method of in situ amplification of DNA of interest in tissue sections, has been subsequently developed and mainly used to investigate tissues infected with viruses. A combination of reverse transcription and PCR has been utilized to detect mRNA of interest in situ. The technique of in situ PCR is promising for a wide variety of applications in basic histology and histopathology.

Original language	English
Pages (from-to)	255-258
Number of pages	4
Journal	Acta Histochemica et Cytochemica
Volume	31
Issue number	4
DOIs	https://doi.org/10.1267/ahc.31.255
Publication status	Published - 1998
Externally published	Yes

Keywords

DNA amplification
Gene expression
In situ RT/PCR
In situ hybridization

ASJC Scopus subject areas

Pathology and Forensic Medicine
Biochemistry
Physiology
Histology
Cell Biology

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10.1267/ahc.31.255

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T2 - In situ PCR

AU - Hiraoka, Yoshiki

AU - Ogawa, Motoyuki

AU - Sakai, Yukinao

AU - Aiso, Sadakazu

PY - 1998

Y1 - 1998

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AB - The most innovative technique introduced recently into the field of biological study is the polymerase chain reaction (PCR) which can amplify even single-copy DNA sequences to high levels. In situ PCR, which is a method of in situ amplification of DNA of interest in tissue sections, has been subsequently developed and mainly used to investigate tissues infected with viruses. A combination of reverse transcription and PCR has been utilized to detect mRNA of interest in situ. The technique of in situ PCR is promising for a wide variety of applications in basic histology and histopathology.

KW - DNA amplification

KW - Gene expression

KW - In situ RT/PCR

KW - In situ hybridization

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JF - Acta Histochemica et Cytochemica

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The cutting edge of histochemical technique: In situ PCR

Abstract

Keywords

ASJC Scopus subject areas

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