The cutting edge of histochemical technique: In situ PCR

Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Sadakazu Aiso

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The most innovative technique introduced recently into the field of biological study is the polymerase chain reaction (PCR) which can amplify even single-copy DNA sequences to high levels. In situ PCR, which is a method of in situ amplification of DNA of interest in tissue sections, has been subsequently developed and mainly used to investigate tissues infected with viruses. A combination of reverse transcription and PCR has been utilized to detect mRNA of interest in situ. The technique of in situ PCR is promising for a wide variety of applications in basic histology and histopathology.

Original languageEnglish
Pages (from-to)255-258
Number of pages4
JournalActa Histochemica et Cytochemica
Volume31
Issue number4
DOIs
Publication statusPublished - 1998 Jan 1

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Keywords

  • DNA amplification
  • Gene expression
  • In situ RT/PCR
  • In situ hybridization

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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