The cutting edge of histochemical technique: In situ PCR

Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Sadakazu Aiso

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The most innovative technique introduced recently into the field of biological study is the polymerase chain reaction (PCR) which can amplify even single-copy DNA sequences to high levels. In situ PCR, which is a method of in situ amplification of DNA of interest in tissue sections, has been subsequently developed and mainly used to investigate tissues infected with viruses. A combination of reverse transcription and PCR has been utilized to detect mRNA of interest in situ. The technique of in situ PCR is promising for a wide variety of applications in basic histology and histopathology.

Original languageEnglish
Pages (from-to)255-258
Number of pages4
JournalActa Histochemica et Cytochemica
Volume31
Issue number4
DOIs
Publication statusPublished - 1998 Jan 1
Externally publishedYes

Fingerprint

Polymerase chain reaction
Tissue
Histology
DNA sequences
Transcription
Viruses
Amplification
Messenger RNA
DNA

Keywords

  • DNA amplification
  • Gene expression
  • In situ hybridization
  • In situ RT/PCR

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

Cite this

The cutting edge of histochemical technique : In situ PCR. / Hiraoka, Yoshiki; Ogawa, Motoyuki; Sakai, Yukinao; Aiso, Sadakazu.

In: Acta Histochemica et Cytochemica, Vol. 31, No. 4, 01.01.1998, p. 255-258.

Research output: Contribution to journalArticle

Hiraoka, Yoshiki ; Ogawa, Motoyuki ; Sakai, Yukinao ; Aiso, Sadakazu. / The cutting edge of histochemical technique : In situ PCR. In: Acta Histochemica et Cytochemica. 1998 ; Vol. 31, No. 4. pp. 255-258.
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