The effect of excess β-chain synthesis on cell-surface expression of allele-mismatched class II heterodimers in vivo

Susan Gilfillan, Sadakazu Aiso, Sara A. Michie, Hugh O. Mcdevitt

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11 Citations (Scopus)

Abstract

We have recently described 12 lines of H-2s/s mice carrying from 1 to 65 copies of an Aβ k transgene. The transgene was coexpressed with the endogenous allele, and Aβ k mRNA expression correlated well with transgene copy number. Overexpression of the transgene was associated with a variety of defects, including a significant reduction in I-A cell-surface expression. In this paper, we assess the effect of increased levels of Aβ k mRNA synthesis on I-A cell-surface expression in these mice. Crossing representatives from several lines of Aβ k mice to Aα k transgenic mice demonstrated that the Aβ k mRNA was translated and expressed at high levels on the cell surface in association with Aα k. In H-2s/s (Aα s/Aβ s) mice carrying >10 copies of the Aβ k transgene, excess Aβ k mRNA and protein synthesis did drive cell-surface expression of the less favored Aα s/Aβ k heterodimers. However, the highest levels of Aβ k detected on the cell surface were only 50-70% of those observed in [B10.A(4R) x nontransgenic]F1 controls. Maximum levels of Aα s/Aβ k cell-surface expression were accompanied by a significant reduction in Aα s/Aβ s expression. Unpaired and improperly paired complexes were not detected intracellularly and appeared to be degraded quite rapidly. Thus, only a fraction of the chains competing for pairing reached the cell surface under conditions of asymmetric chain synthesis in these mice. This markedly reduced total Ia cell-surface levels in mice carrying >10 copies of the Aβ k transgene.

Original languageEnglish
Pages (from-to)7314-7318
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number18
DOIs
Publication statusPublished - 1990 Jan 1
Externally publishedYes

Keywords

  • Chain pairing
  • Transgenic mice

ASJC Scopus subject areas

  • General

Cite this

The effect of excess β-chain synthesis on cell-surface expression of allele-mismatched class II heterodimers in vivo. / Gilfillan, Susan; Aiso, Sadakazu; Michie, Sara A.; Mcdevitt, Hugh O.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 18, 01.01.1990, p. 7314-7318.

Research output: Contribution to journalArticle

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