The effects of reactive oxygen species on amphibian aging

Keiko Kashiwagi, Tadashi Shinkai, Eiji Kajii, Akihiko Kashiwagi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To clarify the role of reactive oxygen species (ROS) in the aging process of amphibians, antioxidant enzyme activity and indexes of ROS damage were investigated biochemically using the livers of 3- and 10-year-old Rana nigromaculata frog males and females. Findings revealed no significant difference in survival rate between males and females. Antioxidant enzyme activity displayed an age-related decline. Superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activity in 10-year-old liver decreased 40-80% from 3-year-old liver levels. In contrast, urate oxidase activity in the 10-year-old liver increased more than 200% from 3-year-old liver levels. At the same time levels of ROS damage, including the concentration of inorganic peroxide and thiobarbituric acid reactive substances (TBARS), greatly increased with age. Liver catalase from 10-year-old frogs proved to be more susceptible to aminotriazole and urea, losing approximately 80% of its original activity after 30 min of treatment. It seems likely that liver catalase in older frogs has diverged from liver catalase in younger frogs through oxidative modification. These findings suggest that a decrease in the activity of antioxidant enzymes over time results in increased levels of ROS damage in the livers of older frogs.

Original languageEnglish
Pages (from-to)197-205
Number of pages9
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume140
Issue number2
DOIs
Publication statusPublished - 2005 Feb
Externally publishedYes

Fingerprint

Liver
Reactive Oxygen Species
Aging of materials
Catalase
Antioxidants
Enzyme activity
Urate Oxidase
Amitrole
Thiobarbituric Acid Reactive Substances
Peroxides
Glutathione Peroxidase
Superoxide Dismutase
Urea
Enzymes

Keywords

  • Antioxidant defense system
  • Antioxidant enzymes
  • Frog aging
  • Frog liver
  • Oxidative damage
  • Reactive oxygen species
  • ROS detoxification mechanism

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

The effects of reactive oxygen species on amphibian aging. / Kashiwagi, Keiko; Shinkai, Tadashi; Kajii, Eiji; Kashiwagi, Akihiko.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 140, No. 2, 02.2005, p. 197-205.

Research output: Contribution to journalArticle

Kashiwagi, Keiko ; Shinkai, Tadashi ; Kajii, Eiji ; Kashiwagi, Akihiko. / The effects of reactive oxygen species on amphibian aging. In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology. 2005 ; Vol. 140, No. 2. pp. 197-205.
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