The monoamine regulon including syntheses of arylsulfatase and monoamine oxidase in bacteria

Yoshikatsu Murooka, Hiroyuki Azakami, Mitsuo Yamashita

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Bacterial cells respond to monoamine compounds, such as tyramine, dopamine, octopamine, or norepinephrine, and induce the syntheses of tyramine oxidase encoded by tynA and monoamine oxidase encoded by maoA. These monoamine compounds also derepress the synthesis of ats A- specified arylsulfatase that is repressed by sulfur compounds. These complex mechanisms of regulons regulated by monoamine and sulfur compounds has been analyzed by cloning and characterization of genes that are involved in the repression and derepression of the synthesis of arylsulfatase. The atsA gene forms an operon with the atsB gene, which encodes an activator of the expression of atsA. The negative regulator gene for arylsulfatase was found to code for dihydrofolate reductase (folA). The maoA gene forms an operon with the maoC gene, which has similarity to a dehydrogenase involved in the tyramine metabolism. The moaF gene encoding a 30-kDa protein, which is induced by tyramine, also forms an operon with the moaE gene. Finally, the moaR gene, which is induced by monoamine, was found to play a central role in the positive regulation of the expression of the monoamine regulon (moa) including the atsBA, maoCA, moaEF, and tyn operons. The moaR expression is subject to autogenous regulation and to cAMP-CRP control. The MoaR protein has a helix-turn-helix motif in its C terminus. Thus, the MoaR protein probably regulates the operons by binding to the regulatory region of the moa regulon.

Original languageEnglish
Pages (from-to)935-941
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume60
Issue number6
Publication statusPublished - 1996 Jun
Externally publishedYes

Fingerprint

Arylsulfatases
amine oxidase (flavin-containing)
regulon
monoamines
arylsulfatase
Monoamine Oxidase
Bacteria
Genes
operon
synthesis
bacteria
Tyramine
tyramine
genes
Sulfur Compounds
helix-turn-helix motifs
sulfur
Octopamine
dihydrofolate reductase
octopamine

Keywords

  • arylsulfatase
  • Klebsiella
  • monoamine oxidase
  • monoamine regulon
  • sulfate regulon

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

The monoamine regulon including syntheses of arylsulfatase and monoamine oxidase in bacteria. / Murooka, Yoshikatsu; Azakami, Hiroyuki; Yamashita, Mitsuo.

In: Bioscience, Biotechnology and Biochemistry, Vol. 60, No. 6, 06.1996, p. 935-941.

Research output: Contribution to journalArticle

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