The observations of the flow behavior and distribution of red blood cells flowing through a micro-network channel

Fen Li, Ruiqing Hu, Yamada Takashi, Ying He, Naoki Ono

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The structure of vascular network in solid tumor is extremely disordered and non-uniformed. These characteristics result in the complexity and diversity of the blood flow in tumor microcirculation which eventually make drug delivery and targeted therapy difficult in solid tumor. In order to investigate the influence of tumor microvascular network on the blood flow, a vertical interconnected micro-network channel was fabricated by soft lithographical method in this work, designed to simulate the expanding, multi-branched and multi-interconnected tumor vascular network. Employing the micro-flow-system, the red blood cell (RBC) suspension was injected into micro network channel at a certain speed. Inverted microscope was used to observe the migration of RBCs and the sequential images were recorded. PIV-lab package of Matlab and the tool box of the high-speed video camera were used to process the image data. The results show that, hemotocrit (Hct) level of RBC suspension is the main factor to affect the flow and distribution of RBCs in the micro-network. The trajectories of RBCs in the micro-network vary with different Hcts. When Hct level is as low as 1%, the RBCs in the micro-network flow only along the axial direction of the channels, while Hct level becomes higher, some RBCs will flow across the radial channel and the two types of RBC flow trajectories appeared. Furthermore, at the same inlet flow rate, the speeds of RBCs in the micro-network show difference with different Hct levels. The velocities of RBCs with 3% and 5% Hct levels are evidently higher than those of RBCs with 1% Hct level.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalLixue Xuebao/Chinese Journal of Theoretical and Applied Mechanics
Volume46
Issue number1
DOIs
Publication statusPublished - 2014 Jan

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Tumors
Blood
Cells
Trajectories
Microcirculation
Inlet flow
High speed cameras
Video cameras
Drug delivery
Microscopes
Flow rate

Keywords

  • Flow behavior
  • Microfluidics devices
  • RBC distribution
  • RBC suspension
  • Tumor vasculature

ASJC Scopus subject areas

  • Applied Mathematics
  • Computational Mechanics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

The observations of the flow behavior and distribution of red blood cells flowing through a micro-network channel. / Li, Fen; Hu, Ruiqing; Takashi, Yamada; He, Ying; Ono, Naoki.

In: Lixue Xuebao/Chinese Journal of Theoretical and Applied Mechanics, Vol. 46, No. 1, 01.2014, p. 1-9.

Research output: Contribution to journalArticle

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