The sensitivity of Chlamydomonas photoreceptor is optimized for the frequency of cell body rotation

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

For phototactic migration, Chlamydomonas scans the surrounding light environment by rotating the cell body with an eyespot located on the equator. The intensity of the light signal received by the eyespot should therefore change cyclically at the frequency of the cell body rotation. In this study, the response of the photoreceptor to cyclically changing light stimuli was analyzed using immotile mutant cells. To simulate the light intensity change perceived by a rotating cell, light stimuli were applied that consisted of a light phase with the intensity changing similar to a half cycle of a sine wave and a dark phase of the same length. The fluence rate at the peak of the sine wave was of the order of 1019 photons m-2 s-1, i.e. high intensity at which phototaxis is saturated. A photoreceptor current (PRC) was produced at the onset of each light phase. Interestingly, its amplitude varied depending on the frequency and was largest at 1-5 Hz, a frequency range similar to the frequency of cell body rotation. Experiments on the kinetics of the PRC indicate that the response was small at low frequency because of the inactivation of the PRC before full activation. In contrast, at high frequency the PRC was suppressed by adaptation to the repetitive stimuli. These characteristic kinetics of the PRC should be important for Chlamydomonas cells to extract information from the signals generated by the cell body rotation.

Original languageEnglish
Pages (from-to)665-672
Number of pages8
JournalPlant and Cell Physiology
Volume42
Issue number6
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Chlamydomonas
photoreceptors
cells
light intensity
kinetics
phototaxis
inactivation
mutants

Keywords

  • Chlamydomonas
  • Green algae
  • Photoreceptor current
  • Phototaxis
  • Rhodopsin

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

The sensitivity of Chlamydomonas photoreceptor is optimized for the frequency of cell body rotation. / Yoshimura, Kenjiro; Kamiya, R.

In: Plant and Cell Physiology, Vol. 42, No. 6, 2001, p. 665-672.

Research output: Contribution to journalArticle

@article{b8165ff1df8d44f1aef0983285169b24,
title = "The sensitivity of Chlamydomonas photoreceptor is optimized for the frequency of cell body rotation",
abstract = "For phototactic migration, Chlamydomonas scans the surrounding light environment by rotating the cell body with an eyespot located on the equator. The intensity of the light signal received by the eyespot should therefore change cyclically at the frequency of the cell body rotation. In this study, the response of the photoreceptor to cyclically changing light stimuli was analyzed using immotile mutant cells. To simulate the light intensity change perceived by a rotating cell, light stimuli were applied that consisted of a light phase with the intensity changing similar to a half cycle of a sine wave and a dark phase of the same length. The fluence rate at the peak of the sine wave was of the order of 1019 photons m-2 s-1, i.e. high intensity at which phototaxis is saturated. A photoreceptor current (PRC) was produced at the onset of each light phase. Interestingly, its amplitude varied depending on the frequency and was largest at 1-5 Hz, a frequency range similar to the frequency of cell body rotation. Experiments on the kinetics of the PRC indicate that the response was small at low frequency because of the inactivation of the PRC before full activation. In contrast, at high frequency the PRC was suppressed by adaptation to the repetitive stimuli. These characteristic kinetics of the PRC should be important for Chlamydomonas cells to extract information from the signals generated by the cell body rotation.",
keywords = "Chlamydomonas, Green algae, Photoreceptor current, Phototaxis, Rhodopsin",
author = "Kenjiro Yoshimura and R. Kamiya",
year = "2001",
language = "English",
volume = "42",
pages = "665--672",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - The sensitivity of Chlamydomonas photoreceptor is optimized for the frequency of cell body rotation

AU - Yoshimura, Kenjiro

AU - Kamiya, R.

PY - 2001

Y1 - 2001

N2 - For phototactic migration, Chlamydomonas scans the surrounding light environment by rotating the cell body with an eyespot located on the equator. The intensity of the light signal received by the eyespot should therefore change cyclically at the frequency of the cell body rotation. In this study, the response of the photoreceptor to cyclically changing light stimuli was analyzed using immotile mutant cells. To simulate the light intensity change perceived by a rotating cell, light stimuli were applied that consisted of a light phase with the intensity changing similar to a half cycle of a sine wave and a dark phase of the same length. The fluence rate at the peak of the sine wave was of the order of 1019 photons m-2 s-1, i.e. high intensity at which phototaxis is saturated. A photoreceptor current (PRC) was produced at the onset of each light phase. Interestingly, its amplitude varied depending on the frequency and was largest at 1-5 Hz, a frequency range similar to the frequency of cell body rotation. Experiments on the kinetics of the PRC indicate that the response was small at low frequency because of the inactivation of the PRC before full activation. In contrast, at high frequency the PRC was suppressed by adaptation to the repetitive stimuli. These characteristic kinetics of the PRC should be important for Chlamydomonas cells to extract information from the signals generated by the cell body rotation.

AB - For phototactic migration, Chlamydomonas scans the surrounding light environment by rotating the cell body with an eyespot located on the equator. The intensity of the light signal received by the eyespot should therefore change cyclically at the frequency of the cell body rotation. In this study, the response of the photoreceptor to cyclically changing light stimuli was analyzed using immotile mutant cells. To simulate the light intensity change perceived by a rotating cell, light stimuli were applied that consisted of a light phase with the intensity changing similar to a half cycle of a sine wave and a dark phase of the same length. The fluence rate at the peak of the sine wave was of the order of 1019 photons m-2 s-1, i.e. high intensity at which phototaxis is saturated. A photoreceptor current (PRC) was produced at the onset of each light phase. Interestingly, its amplitude varied depending on the frequency and was largest at 1-5 Hz, a frequency range similar to the frequency of cell body rotation. Experiments on the kinetics of the PRC indicate that the response was small at low frequency because of the inactivation of the PRC before full activation. In contrast, at high frequency the PRC was suppressed by adaptation to the repetitive stimuli. These characteristic kinetics of the PRC should be important for Chlamydomonas cells to extract information from the signals generated by the cell body rotation.

KW - Chlamydomonas

KW - Green algae

KW - Photoreceptor current

KW - Phototaxis

KW - Rhodopsin

UR - http://www.scopus.com/inward/record.url?scp=0034958410&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034958410&partnerID=8YFLogxK

M3 - Article

C2 - 11427687

AN - SCOPUS:0034958410

VL - 42

SP - 665

EP - 672

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 6

ER -