Theaflavins decrease skeletal muscle wasting in disuse atrophy induced by hindlimb suspension in mice

Kenta Suzuki, Nayuta Hirashima, Yasuyuki Fujii, Taiki Fushimi, Ayaka Yamamoto, Tomoya Ueno, Ryota Akagi, Naomi Osakabe

Research output: Contribution to journalArticlepeer-review

Abstract

We previously found that a single dose of theaflavins induced skeletal muscle metabolic changes. In this study, we examined the effect of theaflavins on disuse muscle atrophy model mice by hindlimb suspension. Mice were assigned to 4 groups; ground- vehicle, ground-theaflavins, suspension-vehicle, and suspension- theaflavins, dosed with theaflavins (250 mg/kg/day) for 2 weeks. The peak of myotube size of cross sectional area was significantly moved to the smaller side in the suspension-vehicle group compared with the ground-vehicle group, and these shifts were significantly reduced by the treatment with theaflavins in both soleus and extensor digitorum longus. The level of phosphorylated eukaryotic translation initiation factor 4E-binding protein (4EBP)-1, located downstream of the Akt/mTOR pathway, was significantly different between suspension-vehicle and suspension-theaflavins in soleus. The ratio of forkhead box O (FoxO) 3a to phosphorylated FoxO3a significantly increased in soleus or tended to rise in extensor digitorum longus of suspension-vehicle group compared with ground-vehicle. In contrast, these changes were not observed in suspension-theaflavins group. These results suggested that theaflavins inhibited the progress of disuse muscle atrophy through modulation of protein metabolism.

Original languageEnglish
Pages (from-to)228-234
Number of pages7
JournalJournal of Clinical Biochemistry and Nutrition
Volume68
Issue number3
DOIs
Publication statusPublished - 2021

Keywords

  • FoxO3a
  • Hindlimb suspension
  • Skeletal muscle atrophy
  • Theaflavins

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry

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