Thermophilic Talaromyces emersonii Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase Bioanode for Biosensor and Biofuel Cell Applications

Hisanori Iwasa, Atsunori Hiratsuka, Kenji Yokoyama, Hirotaka Uzawa, Kouhei Orihara, Hitoshi Muguruma

Research output: Contribution to journalArticle

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Abstract

Flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase (GDH) was identified and cloned from thermophilic filamentous fungi Talaromyces emersonii using the homology cloning method. A direct electron transfer bioanode composed of T. emersonii FAD-GDH and a single-walled carbon nanotube was produced. Enzymes from thermophilic microorganisms generally have low activity at ambient temperature; however, the T. emersonii FAD-GDH bioanode exhibits a large anodic current due to the enzymatic reaction (1 mA cm-2) at ambient temperature. Furthermore, the T. emersonii FAD-GDH bioanode worked at 70 °C for 12 h. This is the first report of a bioanode with a glucose-catalyzing enzyme from a thermophilic microorganism that has potential for biosensor and biofuel cell applications. In addition, we demonstrate how the glycoforms of T. emersonii FAD-GDHs expressed by various hosts influence the electrochemical properties of the bioanode.

LanguageEnglish
Pages1660-1665
Number of pages6
JournalACS Omega
Volume2
Issue number4
DOIs
StatePublished - 2017 Apr 30

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Glucose 1-Dehydrogenase
Biological fuel cells
Flavin-Adenine Dinucleotide
Biosensors
Glucose
Microorganisms
Enzymes
Cloning
Single-walled carbon nanotubes (SWCN)
Fungi
Electrochemical properties
Oxidoreductases
Temperature
Electrons

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

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Thermophilic Talaromyces emersonii Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase Bioanode for Biosensor and Biofuel Cell Applications. / Iwasa, Hisanori; Hiratsuka, Atsunori; Yokoyama, Kenji; Uzawa, Hirotaka; Orihara, Kouhei; Muguruma, Hitoshi.

In: ACS Omega, Vol. 2, No. 4, 30.04.2017, p. 1660-1665.

Research output: Contribution to journalArticle

Iwasa, Hisanori ; Hiratsuka, Atsunori ; Yokoyama, Kenji ; Uzawa, Hirotaka ; Orihara, Kouhei ; Muguruma, Hitoshi. / Thermophilic Talaromyces emersonii Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase Bioanode for Biosensor and Biofuel Cell Applications. In: ACS Omega. 2017 ; Vol. 2, No. 4. pp. 1660-1665
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