Abstract
Flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase (GDH) was identified and cloned from thermophilic filamentous fungi Talaromyces emersonii using the homology cloning method. A direct electron transfer bioanode composed of T. emersonii FAD-GDH and a single-walled carbon nanotube was produced. Enzymes from thermophilic microorganisms generally have low activity at ambient temperature; however, the T. emersonii FAD-GDH bioanode exhibits a large anodic current due to the enzymatic reaction (1 mA cm-2) at ambient temperature. Furthermore, the T. emersonii FAD-GDH bioanode worked at 70 °C for 12 h. This is the first report of a bioanode with a glucose-catalyzing enzyme from a thermophilic microorganism that has potential for biosensor and biofuel cell applications. In addition, we demonstrate how the glycoforms of T. emersonii FAD-GDHs expressed by various hosts influence the electrochemical properties of the bioanode.
| Original language | English |
|---|---|
| Pages (from-to) | 1660-1665 |
| Number of pages | 6 |
| Journal | ACS Omega |
| Volume | 2 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2017 Apr 30 |
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ASJC Scopus subject areas
- Chemical Engineering(all)
- Chemistry(all)
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Thermophilic Talaromyces emersonii Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase Bioanode for Biosensor and Biofuel Cell Applications. / Iwasa, Hisanori; Hiratsuka, Atsunori; Yokoyama, Kenji; Uzawa, Hirotaka; Orihara, Kouhei; Muguruma, Hitoshi.
In: ACS Omega, Vol. 2, No. 4, 30.04.2017, p. 1660-1665.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Thermophilic Talaromyces emersonii Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase Bioanode for Biosensor and Biofuel Cell Applications
AU - Iwasa, Hisanori
AU - Hiratsuka, Atsunori
AU - Yokoyama, Kenji
AU - Uzawa, Hirotaka
AU - Orihara, Kouhei
AU - Muguruma, Hitoshi
PY - 2017/4/30
Y1 - 2017/4/30
N2 - Flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase (GDH) was identified and cloned from thermophilic filamentous fungi Talaromyces emersonii using the homology cloning method. A direct electron transfer bioanode composed of T. emersonii FAD-GDH and a single-walled carbon nanotube was produced. Enzymes from thermophilic microorganisms generally have low activity at ambient temperature; however, the T. emersonii FAD-GDH bioanode exhibits a large anodic current due to the enzymatic reaction (1 mA cm-2) at ambient temperature. Furthermore, the T. emersonii FAD-GDH bioanode worked at 70 °C for 12 h. This is the first report of a bioanode with a glucose-catalyzing enzyme from a thermophilic microorganism that has potential for biosensor and biofuel cell applications. In addition, we demonstrate how the glycoforms of T. emersonii FAD-GDHs expressed by various hosts influence the electrochemical properties of the bioanode.
AB - Flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase (GDH) was identified and cloned from thermophilic filamentous fungi Talaromyces emersonii using the homology cloning method. A direct electron transfer bioanode composed of T. emersonii FAD-GDH and a single-walled carbon nanotube was produced. Enzymes from thermophilic microorganisms generally have low activity at ambient temperature; however, the T. emersonii FAD-GDH bioanode exhibits a large anodic current due to the enzymatic reaction (1 mA cm-2) at ambient temperature. Furthermore, the T. emersonii FAD-GDH bioanode worked at 70 °C for 12 h. This is the first report of a bioanode with a glucose-catalyzing enzyme from a thermophilic microorganism that has potential for biosensor and biofuel cell applications. In addition, we demonstrate how the glycoforms of T. emersonii FAD-GDHs expressed by various hosts influence the electrochemical properties of the bioanode.
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U2 - 10.1021/acsomega.7b00277
DO - 10.1021/acsomega.7b00277
M3 - Article
VL - 2
SP - 1660
EP - 1665
JO - ACS Omega
JF - ACS Omega
SN - 2470-1343
IS - 4
ER -