Thermotaxis in Chlamydomonas is brought about by membrane excitation and controlled by redox conditions

Masaya Sekiguchi, Shigetoshi Kameda, Satoshi Kurosawa, Megumi Yoshida, Kenjiro Yoshimura

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Abstract

Temperature is physiologically critical for all living organisms, which cope with temperature stress using metabolic and behavioral responses. In unicellular and some multicellular organisms, thermotaxis is a behavioral response to avoid stressful thermal environments and promote accumulation in an optimal thermal environment. In this study, we examined whether Chlamydomonas reinhardtii, a unicellular green alga, demonstrated thermotaxis. We found that between 10 °C and 30 °C, Chlamydomonas cells migrated toward lower temperatures independent of cultivation temperature. Interestingly, when we applied reagents to change intracellular reduction-oxidation (redox) conditions, we saw that thermotaxis was enhanced, suppressed, or reversed, depending on the redox conditions and cultivation temperature. Thermotaxis was almost absent in ppr2 and ppr3 mutants, which cannot swim backward because of a defect in generating calcium current in flagella. The frequency of spontaneous backward swimming was lower at more favorable temperature, suggesting a pivotal role of spontaneous backward swimming generated by flagellar membrane excitation.

Original languageEnglish
Article number16114
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

ASJC Scopus subject areas

  • General

Cite this

Thermotaxis in Chlamydomonas is brought about by membrane excitation and controlled by redox conditions. / Sekiguchi, Masaya; Kameda, Shigetoshi; Kurosawa, Satoshi; Yoshida, Megumi; Yoshimura, Kenjiro.

In: Scientific Reports, Vol. 8, No. 1, 16114, 01.12.2018.

Research output: Contribution to journalArticle

Sekiguchi, Masaya ; Kameda, Shigetoshi ; Kurosawa, Satoshi ; Yoshida, Megumi ; Yoshimura, Kenjiro. / Thermotaxis in Chlamydomonas is brought about by membrane excitation and controlled by redox conditions. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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