Abstract
The authors have proposed a new type of ultrasonic microscopy for biological tissue characterization. The system is driven by a nanosecond pulse voltage, the generated acoustic wave being reflected at the front and rear side of the sliced tissue. In this report, a time-frequency analysis was applied to determine the sound speed thorough the tissue. Frequency dependence of sound speed was obtained with a myocardium of a rat sliced into 10 μm. As the reflected waveform had a significant amount of oscillating component, the waveform was once subjected to the deconvolution process. As the result, two reflections were clearly separated in time domain. Then these two reflections were separately analyzed by time-frequency analysis. Each reflection was extracted by using a proper window function. Phase angles of these reflections at the same frequency were compared. A sound speed micrograph at an arbitrary frequency in between 50 and 150 MHz was successfully obtained. A tendency was found that the sound speed slightly increases with frequency.
| Original language | English |
|---|---|
| Pages (from-to) | 717-722 |
| Number of pages | 6 |
| Journal | Ultrasonics |
| Volume | 42 |
| Issue number | 1-9 |
| DOIs | |
| Publication status | Published - 2004 Apr |
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Keywords
- Signal processing
- Sound speed
- Time-frequency analysis
- Tissue characterization
ASJC Scopus subject areas
- Safety, Risk, Reliability and Quality
- Acoustics and Ultrasonics
Cite this
Time-frequency analysis for pulse driven ultrasonic microscopy for biological tissue characterization. / Hozumi, N.; Yamashita, R.; Lee, C. K.; Nagao, M.; Kobayashi, K.; Saijo, Y.; Tanaka, M.; Tanaka, Naohiko; Ohtsuki, S.
In: Ultrasonics, Vol. 42, No. 1-9, 04.2004, p. 717-722.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Time-frequency analysis for pulse driven ultrasonic microscopy for biological tissue characterization
AU - Hozumi, N.
AU - Yamashita, R.
AU - Lee, C. K.
AU - Nagao, M.
AU - Kobayashi, K.
AU - Saijo, Y.
AU - Tanaka, M.
AU - Tanaka, Naohiko
AU - Ohtsuki, S.
PY - 2004/4
Y1 - 2004/4
N2 - The authors have proposed a new type of ultrasonic microscopy for biological tissue characterization. The system is driven by a nanosecond pulse voltage, the generated acoustic wave being reflected at the front and rear side of the sliced tissue. In this report, a time-frequency analysis was applied to determine the sound speed thorough the tissue. Frequency dependence of sound speed was obtained with a myocardium of a rat sliced into 10 μm. As the reflected waveform had a significant amount of oscillating component, the waveform was once subjected to the deconvolution process. As the result, two reflections were clearly separated in time domain. Then these two reflections were separately analyzed by time-frequency analysis. Each reflection was extracted by using a proper window function. Phase angles of these reflections at the same frequency were compared. A sound speed micrograph at an arbitrary frequency in between 50 and 150 MHz was successfully obtained. A tendency was found that the sound speed slightly increases with frequency.
AB - The authors have proposed a new type of ultrasonic microscopy for biological tissue characterization. The system is driven by a nanosecond pulse voltage, the generated acoustic wave being reflected at the front and rear side of the sliced tissue. In this report, a time-frequency analysis was applied to determine the sound speed thorough the tissue. Frequency dependence of sound speed was obtained with a myocardium of a rat sliced into 10 μm. As the reflected waveform had a significant amount of oscillating component, the waveform was once subjected to the deconvolution process. As the result, two reflections were clearly separated in time domain. Then these two reflections were separately analyzed by time-frequency analysis. Each reflection was extracted by using a proper window function. Phase angles of these reflections at the same frequency were compared. A sound speed micrograph at an arbitrary frequency in between 50 and 150 MHz was successfully obtained. A tendency was found that the sound speed slightly increases with frequency.
KW - Signal processing
KW - Sound speed
KW - Time-frequency analysis
KW - Tissue characterization
UR - http://www.scopus.com/inward/record.url?scp=1642585904&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1642585904&partnerID=8YFLogxK
U2 - 10.1016/j.ultras.2003.11.005
DO - 10.1016/j.ultras.2003.11.005
M3 - Article
VL - 42
SP - 717
EP - 722
JO - Ultrasonics
JF - Ultrasonics
SN - 0041-624X
IS - 1-9
ER -