Transient measurement of glucose using on-off controllable enzyme electrode with polypyrrole membrane

Takehiro Miyasaka, Yasuo Yoshimi, Kiyotaka Sakai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A glucose sensor, using glucose oxidase immobilized in an electrically conductive polymer membrane, is developed for transient-mode measurement by on-off reaction control in the present work. An implantable glucose sensor loses its stability under the skin by fouling resulting from fibroblast adhesion on the glucose oxidase-immobilized membrane. Change in glucose distribution inside the membrane and in the vicinity of the surface of the membrane resulting from the fouling makes the measurement of the glucose concentration unstable. A technique of measuring transient current of the glucose sensor after activation of glucose oxidase may hardly be affected by the fouling as oxidation of coenzyme of glucose oxidase is rapid and the transient current is sensitive to glucose concentration. A glucose sensor with glucose oxidase immobilized in an electrically conducive polypyrrole membrane was fabricated. The activity of the glucose oxidase is switched on and off by a stepwise change of potential of the polypyrrole membrane from 0.15 V to 0.45 V versus a saturated calomel electrode. The two conditions described above can be confirmed by measuring transient current. Current increased momentarily after activation of glucose oxidase and decreased moderately after that. The oxidation and reduction of glucose oxidase is sufficiently rapid, and current intensity depends on glucose concentration in the test solution.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalJournal of Chemical Engineering of Japan
Volume31
Issue number1
Publication statusPublished - 1998 Feb

Fingerprint

Enzyme electrodes
Glucose Oxidase
Glucose oxidase
Polypyrroles
Glucose
Glucose sensors
Membranes
Fouling
Chemical activation
Coenzymes
Oxidation
polypyrrole
Fibroblasts
Skin
Polymers
Adhesion
Electrodes

Keywords

  • Biochemical Engineering
  • Enzyme Activity
  • Glucose Oxidase
  • Glucose Sensor
  • Transient Measurement

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Transient measurement of glucose using on-off controllable enzyme electrode with polypyrrole membrane. / Miyasaka, Takehiro; Yoshimi, Yasuo; Sakai, Kiyotaka.

In: Journal of Chemical Engineering of Japan, Vol. 31, No. 1, 02.1998, p. 29-34.

Research output: Contribution to journalArticle

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AB - A glucose sensor, using glucose oxidase immobilized in an electrically conductive polymer membrane, is developed for transient-mode measurement by on-off reaction control in the present work. An implantable glucose sensor loses its stability under the skin by fouling resulting from fibroblast adhesion on the glucose oxidase-immobilized membrane. Change in glucose distribution inside the membrane and in the vicinity of the surface of the membrane resulting from the fouling makes the measurement of the glucose concentration unstable. A technique of measuring transient current of the glucose sensor after activation of glucose oxidase may hardly be affected by the fouling as oxidation of coenzyme of glucose oxidase is rapid and the transient current is sensitive to glucose concentration. A glucose sensor with glucose oxidase immobilized in an electrically conducive polypyrrole membrane was fabricated. The activity of the glucose oxidase is switched on and off by a stepwise change of potential of the polypyrrole membrane from 0.15 V to 0.45 V versus a saturated calomel electrode. The two conditions described above can be confirmed by measuring transient current. Current increased momentarily after activation of glucose oxidase and decreased moderately after that. The oxidation and reduction of glucose oxidase is sufficiently rapid, and current intensity depends on glucose concentration in the test solution.

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