Transient measurement with an enzyme-immobilized electrode undergoing protein adsorption

Satoka Aoyagi; Yusuke Suzuki; Kiyotaka Sakai; Takehiro Miyasaka; Yasuo Yoshimi

doi:10.5796/electrochemistry.69.251

Transient measurement with an enzyme-immobilized electrode undergoing protein adsorption

Satoka Aoyagi, Yusuke Suzuki, Kiyotaka Sakai, Takehiro Miyasaka, Yasuo Yoshimi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A measurement without the influence of protein adsorption on the sensor surface has been developed using transient current of an enzyme-immobilized electrode. Response current decreases due to protein adsorption on the sensor surface and the enzyme-immobilized membrane causing glucose concentration gradient. By measuring glucose concentration in the transient state while controlling GOD activity, it is possible to prevent the influence of protein adsorption. A needle-type electrode glucose sensor on which enzyme is immobilized with a polyurethane membrane, with electrochemical on-off control of enzyme activity, is capable of accurate measurement of glucose concentration in bovine serum at least for 24 hrs.

Original language	English
Pages (from-to)	251-253
Number of pages	3
Journal	Electrochemistry
Volume	69
Issue number	4
DOIs	https://doi.org/10.5796/electrochemistry.69.251
Publication status	Published - 2001 Apr

Keywords

Glucose
Glucose Oxidase
On-off Control
Transient State

ASJC Scopus subject areas

Electrochemistry

Access to Document

10.5796/electrochemistry.69.251

Cite this

@article{b9a40279167745dea49c7f855a93c55f,

title = "Transient measurement with an enzyme-immobilized electrode undergoing protein adsorption",

abstract = "A measurement without the influence of protein adsorption on the sensor surface has been developed using transient current of an enzyme-immobilized electrode. Response current decreases due to protein adsorption on the sensor surface and the enzyme-immobilized membrane causing glucose concentration gradient. By measuring glucose concentration in the transient state while controlling GOD activity, it is possible to prevent the influence of protein adsorption. A needle-type electrode glucose sensor on which enzyme is immobilized with a polyurethane membrane, with electrochemical on-off control of enzyme activity, is capable of accurate measurement of glucose concentration in bovine serum at least for 24 hrs.",

keywords = "Glucose, Glucose Oxidase, On-off Control, Transient State",

author = "Satoka Aoyagi and Yusuke Suzuki and Kiyotaka Sakai and Takehiro Miyasaka and Yasuo Yoshimi",

year = "2001",

month = apr,

doi = "10.5796/electrochemistry.69.251",

language = "English",

volume = "69",

pages = "251--253",

journal = "Electrochemistry",

issn = "1344-3542",

publisher = "Electrochemical Society of Japan",

number = "4",

}

TY - JOUR

T1 - Transient measurement with an enzyme-immobilized electrode undergoing protein adsorption

AU - Aoyagi, Satoka

AU - Suzuki, Yusuke

AU - Sakai, Kiyotaka

AU - Miyasaka, Takehiro

AU - Yoshimi, Yasuo

PY - 2001/4

Y1 - 2001/4

N2 - A measurement without the influence of protein adsorption on the sensor surface has been developed using transient current of an enzyme-immobilized electrode. Response current decreases due to protein adsorption on the sensor surface and the enzyme-immobilized membrane causing glucose concentration gradient. By measuring glucose concentration in the transient state while controlling GOD activity, it is possible to prevent the influence of protein adsorption. A needle-type electrode glucose sensor on which enzyme is immobilized with a polyurethane membrane, with electrochemical on-off control of enzyme activity, is capable of accurate measurement of glucose concentration in bovine serum at least for 24 hrs.

AB - A measurement without the influence of protein adsorption on the sensor surface has been developed using transient current of an enzyme-immobilized electrode. Response current decreases due to protein adsorption on the sensor surface and the enzyme-immobilized membrane causing glucose concentration gradient. By measuring glucose concentration in the transient state while controlling GOD activity, it is possible to prevent the influence of protein adsorption. A needle-type electrode glucose sensor on which enzyme is immobilized with a polyurethane membrane, with electrochemical on-off control of enzyme activity, is capable of accurate measurement of glucose concentration in bovine serum at least for 24 hrs.

KW - Glucose

KW - Glucose Oxidase

KW - On-off Control

KW - Transient State

UR - http://www.scopus.com/inward/record.url?scp=0035302219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035302219&partnerID=8YFLogxK

U2 - 10.5796/electrochemistry.69.251

DO - 10.5796/electrochemistry.69.251

M3 - Article

AN - SCOPUS:0035302219

SN - 1344-3542

VL - 69

SP - 251

EP - 253

JO - Electrochemistry

JF - Electrochemistry

IS - 4

ER -

Transient measurement with an enzyme-immobilized electrode undergoing protein adsorption

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this