Transient measurement with an enzyme-immobilized electrode undergoing protein adsorption

Satoka Aoyagi, Yusuke Suzuki, Kiyotaka Sakai, Takehiro Miyasaka, Yasuo Yoshimi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A measurement without the influence of protein adsorption on the sensor surface has been developed using transient current of an enzyme-immobilized electrode. Response current decreases due to protein adsorption on the sensor surface and the enzyme-immobilized membrane causing glucose concentration gradient. By measuring glucose concentration in the transient state while controlling GOD activity, it is possible to prevent the influence of protein adsorption. A needle-type electrode glucose sensor on which enzyme is immobilized with a polyurethane membrane, with electrochemical on-off control of enzyme activity, is capable of accurate measurement of glucose concentration in bovine serum at least for 24 hrs.

Original languageEnglish
Pages (from-to)251-253
Number of pages3
JournalElectrochemistry
Volume69
Issue number4
DOIs
Publication statusPublished - 2001 Apr

Keywords

  • Glucose
  • Glucose Oxidase
  • On-off Control
  • Transient State

ASJC Scopus subject areas

  • Electrochemistry

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