Use of lac Gene Fusions to Study Regulation of Tyramine Oxidase, Which is Involved in Derepression of Latent Arylsulfatase in Escherichia coli

Mitsuo Yamashita, Yoshikatsu Murooka

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1 Citation (Scopus)

Abstract

Strains with lac fused to each of the arylsulfatase (ats) and tyramine oxidase (tyn) Operons in Escherichia coli were isolated. Synthesis of β-galactosidase in strains with tyn:: lac fusions was induced by tyramine, histamine, tryptamine, dopamine and octopamine, and the induction of the tyn Operon was subject to catabolite and ammonium repressions. These repressions were relieved when the cells were grown with a poor carbon or nitrogen source. No arylsulfatase activity is detected in E. coli strains. Synthesis of β-galactosidase in strains with ats:: lac fusions was repressed by sulfur compounds. The repression was relieved by monoamine compounds, which induced tyramine oxidase synthesis. The inhibition of tyramine oxidase activity by cysteine resulted in a decrease of the derepressed synthesis of β-galactosidase in the ats:: lac fusion. Repressing and derepressing conditions for the tyn Operon prevented and stimulated, respectively, expression of the ats Operon. Thus, the expression of latent arylsulfatase in E. coli seems to be regulated by expression of the tyn Operon.

Original languageEnglish
Pages (from-to)1459-1470
Number of pages12
JournalAgricultural and Biological Chemistry
Volume48
Issue number6
DOIs
Publication statusPublished - 1984
Externally publishedYes

Fingerprint

Arylsulfatases
amine oxidase (flavin-containing)
arylsulfatase
gene fusion
Monoamine Oxidase
Escherichia coli
operon
Fusion reactions
Genes
Galactosidases
galactosidases
synthesis
Octopamine
octopamine
Sulfur Compounds
tryptamine
monoamines
Tyramine
tyramine
histamine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "Use of lac Gene Fusions to Study Regulation of Tyramine Oxidase, Which is Involved in Derepression of Latent Arylsulfatase in Escherichia coli",
abstract = "Strains with lac fused to each of the arylsulfatase (ats) and tyramine oxidase (tyn) Operons in Escherichia coli were isolated. Synthesis of β-galactosidase in strains with tyn:: lac fusions was induced by tyramine, histamine, tryptamine, dopamine and octopamine, and the induction of the tyn Operon was subject to catabolite and ammonium repressions. These repressions were relieved when the cells were grown with a poor carbon or nitrogen source. No arylsulfatase activity is detected in E. coli strains. Synthesis of β-galactosidase in strains with ats:: lac fusions was repressed by sulfur compounds. The repression was relieved by monoamine compounds, which induced tyramine oxidase synthesis. The inhibition of tyramine oxidase activity by cysteine resulted in a decrease of the derepressed synthesis of β-galactosidase in the ats:: lac fusion. Repressing and derepressing conditions for the tyn Operon prevented and stimulated, respectively, expression of the ats Operon. Thus, the expression of latent arylsulfatase in E. coli seems to be regulated by expression of the tyn Operon.",
author = "Mitsuo Yamashita and Yoshikatsu Murooka",
year = "1984",
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language = "English",
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T1 - Use of lac Gene Fusions to Study Regulation of Tyramine Oxidase, Which is Involved in Derepression of Latent Arylsulfatase in Escherichia coli

AU - Yamashita, Mitsuo

AU - Murooka, Yoshikatsu

PY - 1984

Y1 - 1984

N2 - Strains with lac fused to each of the arylsulfatase (ats) and tyramine oxidase (tyn) Operons in Escherichia coli were isolated. Synthesis of β-galactosidase in strains with tyn:: lac fusions was induced by tyramine, histamine, tryptamine, dopamine and octopamine, and the induction of the tyn Operon was subject to catabolite and ammonium repressions. These repressions were relieved when the cells were grown with a poor carbon or nitrogen source. No arylsulfatase activity is detected in E. coli strains. Synthesis of β-galactosidase in strains with ats:: lac fusions was repressed by sulfur compounds. The repression was relieved by monoamine compounds, which induced tyramine oxidase synthesis. The inhibition of tyramine oxidase activity by cysteine resulted in a decrease of the derepressed synthesis of β-galactosidase in the ats:: lac fusion. Repressing and derepressing conditions for the tyn Operon prevented and stimulated, respectively, expression of the ats Operon. Thus, the expression of latent arylsulfatase in E. coli seems to be regulated by expression of the tyn Operon.

AB - Strains with lac fused to each of the arylsulfatase (ats) and tyramine oxidase (tyn) Operons in Escherichia coli were isolated. Synthesis of β-galactosidase in strains with tyn:: lac fusions was induced by tyramine, histamine, tryptamine, dopamine and octopamine, and the induction of the tyn Operon was subject to catabolite and ammonium repressions. These repressions were relieved when the cells were grown with a poor carbon or nitrogen source. No arylsulfatase activity is detected in E. coli strains. Synthesis of β-galactosidase in strains with ats:: lac fusions was repressed by sulfur compounds. The repression was relieved by monoamine compounds, which induced tyramine oxidase synthesis. The inhibition of tyramine oxidase activity by cysteine resulted in a decrease of the derepressed synthesis of β-galactosidase in the ats:: lac fusion. Repressing and derepressing conditions for the tyn Operon prevented and stimulated, respectively, expression of the ats Operon. Thus, the expression of latent arylsulfatase in E. coli seems to be regulated by expression of the tyn Operon.

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