Abstract
We propose a novel detection method for a duplex formation of DNA on a solid substrate. In this method, DNA monolayers are prepared on a gold thin film, and then the substrate is treated by an etching process. Since the DNA monolayer can act as a resist layer, the thickness of the gold film in the regions covered by DNA monolayers is larger than that in the region without the monolayer. Furthermore, the difference in the resistance effect of ss- and ds-DNA monolayers induces the difference in the thickness of the gold thin film after the etching. The resistance effect of the ss-probe DNA monolayer increases as a result of the formation of a duplex with the target DNA. The sequence-specific hybridization adsorption of the target DNA can be detected visually by measuring the light transmittance of the gold film, or with the naked eye. Using this method, we succeeded in detecting two base pair mismatches in a 30-base pair nucleotide.
| Original language | English |
|---|---|
| Pages (from-to) | 556-561 |
| Number of pages | 6 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 105 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2005 Mar 28 |
| Externally published | Yes |
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Keywords
- DNA monolayers
- Etching
- Gold film
- Light transmittance
- Visualization
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
- Electrical and Electronic Engineering
Cite this
Visualization of DNA hybridization on gold thin film by utilizing the resistance effect of DNA monolayer. / Masuda, Takao; Yamaguchi, Akira; Hayashida, Masayuki; Asari-Oi, Fumika; Matsuo, Shigeki; Misawa, Hiroaki.
In: Sensors and Actuators, B: Chemical, Vol. 105, No. 2, 28.03.2005, p. 556-561.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Visualization of DNA hybridization on gold thin film by utilizing the resistance effect of DNA monolayer
AU - Masuda, Takao
AU - Yamaguchi, Akira
AU - Hayashida, Masayuki
AU - Asari-Oi, Fumika
AU - Matsuo, Shigeki
AU - Misawa, Hiroaki
PY - 2005/3/28
Y1 - 2005/3/28
N2 - We propose a novel detection method for a duplex formation of DNA on a solid substrate. In this method, DNA monolayers are prepared on a gold thin film, and then the substrate is treated by an etching process. Since the DNA monolayer can act as a resist layer, the thickness of the gold film in the regions covered by DNA monolayers is larger than that in the region without the monolayer. Furthermore, the difference in the resistance effect of ss- and ds-DNA monolayers induces the difference in the thickness of the gold thin film after the etching. The resistance effect of the ss-probe DNA monolayer increases as a result of the formation of a duplex with the target DNA. The sequence-specific hybridization adsorption of the target DNA can be detected visually by measuring the light transmittance of the gold film, or with the naked eye. Using this method, we succeeded in detecting two base pair mismatches in a 30-base pair nucleotide.
AB - We propose a novel detection method for a duplex formation of DNA on a solid substrate. In this method, DNA monolayers are prepared on a gold thin film, and then the substrate is treated by an etching process. Since the DNA monolayer can act as a resist layer, the thickness of the gold film in the regions covered by DNA monolayers is larger than that in the region without the monolayer. Furthermore, the difference in the resistance effect of ss- and ds-DNA monolayers induces the difference in the thickness of the gold thin film after the etching. The resistance effect of the ss-probe DNA monolayer increases as a result of the formation of a duplex with the target DNA. The sequence-specific hybridization adsorption of the target DNA can be detected visually by measuring the light transmittance of the gold film, or with the naked eye. Using this method, we succeeded in detecting two base pair mismatches in a 30-base pair nucleotide.
KW - DNA monolayers
KW - Etching
KW - Gold film
KW - Light transmittance
KW - Visualization
UR - http://www.scopus.com/inward/record.url?scp=14744289757&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14744289757&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2004.06.015
DO - 10.1016/j.snb.2004.06.015
M3 - Article
VL - 105
SP - 556
EP - 561
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 2
ER -